{"product_id":"filovirus-type-rt-pcr-kit-2-0-ruo-ebola-marburgvirus-rna-detection","title":"Filovirus Type RT-PCR Kit 2.0 RUO","description":"\u003cstyle\u003e\n  .josh-faq-details {\n    border-bottom: 1px solid #e5e7eb;\n    font-family: 'IBM Plex Sans', sans-serif;\n  }\n\n  .josh-faq-details:first-of-type {\n    border-top: 1px solid #e5e7eb;\n  }\n\n  .josh-faq-summary {\n    padding: 20px 0;\n    font-size: 16px;\n    font-weight: 700;\n    color: #000000;\n    cursor: pointer;\n    list-style: none; \/* 隐藏默认三角图标 *\/\n    display: flex;\n    align-items: center;\n    justify-content: space-between;\n    gap: 16px;\n    line-height: 1.4;\n  }\n\n  .josh-faq-summary::-webkit-details-marker {\n    display: none; \/* 隐藏 Webkit 引擎下的默认箭头 *\/\n  }\n\n  .josh-faq-summary:hover {\n    color: #055da9;\n  }\n\n  \/* 自定义加号\/减号图标 *\/\n  .josh-faq-icon {\n    flex-shrink: 0;\n    width: 20px;\n    height: 20px;\n    position: relative;\n  }\n\n  .josh-faq-icon::before,\n  .josh-faq-icon::after {\n    content: '';\n    position: absolute;\n    background: currentColor;\n    border-radius: 2px;\n    transition: transform 0.25s ease, opacity 0.25s ease;\n  }\n\n  .josh-faq-icon::before {\n    width: 100%;\n    height: 2px;\n    top: 50%;\n    left: 0;\n    transform: translateY(-50%);\n  }\n\n  .josh-faq-icon::after {\n    width: 2px;\n    height: 100%;\n    left: 50%;\n    top: 0;\n    transform: translateX(-50%);\n  }\n\n  \/* 展开时将加号变成减号 *\/\n  .josh-faq-details[open] .josh-faq-icon::after {\n    opacity: 0;\n    transform: translateX(-50%) rotate(90deg);\n  }\n\n  .josh-faq-content {\n    padding: 0 0 20px 0;\n    font-size: 16px;\n    line-height: 1.6;\n    color: #333333;\n    max-width: 860px;\n  }\n\n  .josh-faq-content p {\n    margin: 0 0 15px;\n  }\n\n  .josh-faq-content p:last-child {\n    margin-bottom: 0;\n  }\n\n  .josh-faq-content ul {\n    margin: 8px 0 12px;\n    padding-left: 22px;\n  }\n\n  .josh-faq-content li {\n    margin-bottom: 6px;\n    list-style-type: disc;\n  }\n\n  .josh-faq-content a {\n    color: #055da9;\n    text-decoration: none;\n  }\n\n  .josh-faq-content a:hover {\n    text-decoration: underline;\n  }\n\n  @media (max-width: 767px) {\n    .josh-faq-summary {\n      font-size: 15px;\n      padding: 16px 0;\n    }\n\n    .josh-faq-content {\n      font-size: 15px;\n    }\n  }\n\u003c\/style\u003e\n\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eFilovirus Type Probe qRT-PCR Kit is designed for the quantitative detection of Ebola- and Marburgvirus targets using real-time RT-PCR with hydrolysis probes. Purified RNA is reverse transcribed into cDNA and subsequently amplified using primers. During amplification, Taq DNA polymerase cleaves the bound probes, releasing fluorescence signals. The increase in fluorescence measures the amplification process, and Ct values can be used to evaluate relative target abundance among samples.\u003c\/p\u003e\n\u003cp\u003eFilovirus Type Probe qRT-PCR Kit is designed for the quantitative analysis of Ebola- and Marburgvirus targets and differentiation of five Ebolavirus species including Bundibugyo ebolavirus (BDBV), Reston ebolavirus (RESTV), Sudan ebolavirus (SUDV), Taï Forest ebolavirus (TAFV), and Zaire ebolavirus (EBOV).\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e This product is intended for research use only. Not intended for diagnostic or therapeutic use.\u003c\/p\u003e\n\u003ch2\u003eKey Features\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003eOne-step RT-PCR workflow to reduce handling steps and minimize contamination risk.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eHigh sensitivity with an analytical LOD at 100 copies\/reaction.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eHigh specificity with no cross-reaction with RNA from other pathogens.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eIncludes amplification positive control for assay verification.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eCompatible with commonly used RNA purification methods.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eSuitable for research applications including assay development and method evaluation.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eSupports quantitative analysis with a linear range of at least 5 orders of magnitude.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eFor research use only. Not intended for diagnostic or therapeutic use.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eKit Components\u003c\/h2\u003e\n\u003ctable border=\"1\" style=\"width: 90%; border-collapse: collapse;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 20%; text-align: center;\"\u003eComponent Name\u003c\/td\u003e\n\u003ctd style=\"width: 20%; text-align: center;\"\u003eDescription\u003c\/td\u003e\n\u003ctd style=\"width: 20%; text-align: center;\"\u003eSize\u003c\/td\u003e\n\u003ctd style=\"width: 20%; text-align: center;\"\u003eVial\u003c\/td\u003e\n\u003ctd style=\"width: 20%; text-align: center;\"\u003eLid Color\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eEBOV\/MARV qRT-PCR Premix A\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003eTarget-specific qRT-PCR premix\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e550 μL\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e0.5 mL Vial\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003eGreen\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eRESTV\/TAFV qRT-PCR Premix B\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003eTarget-specific qRT-PCR premix\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e550 μL\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e0.5 mL Vial\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003eRed\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eBDBV\/SUDV qRT-PCR Premix C\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003eTarget-specific qRT-PCR premix\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e550 μL\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e0.5 mL Vial\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003eBlue\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eNegative Control\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003eNegative control\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e50 μL\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e0.5 mL Vial\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003eWhite\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eFilovirus Type Positive Control (1E7 copies\/μL)\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003ePositive control\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e50 μL\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e0.5 mL Vial\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003eYellow\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eManual\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003eInstruction booklet\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e1 Set\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003eNA\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003eNA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003eStore at -20 °C.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eThe product is shipped with dry ice to maintain low-temperature conditions and ensure product integrity during transit.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cdiv class=\"josh-faq-container\"\u003e\n\u003cdetails class=\"josh-faq-details\"\u003e\n\u003csummary class=\"josh-faq-summary\"\u003eIs a ROX Dye included with the kit? \u003cspan class=\"josh-faq-icon\"\u003e\u003c\/span\u003e\u003c\/summary\u003e\n\u003cdiv class=\"josh-faq-content\"\u003e\n\u003cp\u003eNo — ROX dye is not included with the kit. We can add a passive reference dye matched to your instrument upon request for $25 USD. To request a ROX dye, please contact us for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/details\u003e\n\u003cdetails class=\"josh-faq-details\"\u003e\n\u003csummary class=\"josh-faq-summary\"\u003eIs an Internal Control included with the kit? \u003cspan class=\"josh-faq-icon\"\u003e\u003c\/span\u003e\u003c\/summary\u003e\n\u003cdiv class=\"josh-faq-content\"\u003e\n\u003cp\u003eYes. This kit includes a pre-mixed Internal Control (IC) system. IC primers and probes are already pre-mixed within each PCR premix (Tube A, B, and C). No additional IC components are required for the PCR reaction setup.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/details\u003e\n\u003cdetails class=\"josh-faq-details\"\u003e\n\u003csummary class=\"josh-faq-summary\"\u003eIs validation data available for the kit? \u003cspan class=\"josh-faq-icon\"\u003e\u003c\/span\u003e\u003c\/summary\u003e\n\u003cdiv class=\"josh-faq-content\"\u003e\n\u003cp\u003eValidation data may be available upon request, depending on the target. Standard validation generated from plasmid-based positive controls can typically be provided for many kits. Validation generated using the actual target material is handled case by case — it requires that we are able to source the material, or that the customer supplies an extracted nucleic acid sample. Please note all kits are intended strictly for research use only. To enquire, contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/details\u003e\n\u003cdetails class=\"josh-faq-details\"\u003e\n\u003csummary class=\"josh-faq-summary\"\u003eWhat is the lead time for all PCR kits? \u003cspan class=\"josh-faq-icon\"\u003e\u003c\/span\u003e\u003c\/summary\u003e\n\u003cdiv class=\"josh-faq-content\"\u003e\n\u003cp\u003eStandard production takes 7–14 days, after which kits ship from our laboratory to our warehouse. Expedited production (~7 days) is available upon request. We also maintain a ready inventory of our most popular kits to reduce wait times.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/details\u003e\n\u003cdetails class=\"josh-faq-details\"\u003e\n\u003csummary class=\"josh-faq-summary\"\u003eCan I customize this kit? \u003cspan class=\"josh-faq-icon\"\u003e\u003c\/span\u003e\u003c\/summary\u003e\n\u003cdiv class=\"josh-faq-content\"\u003e\n\u003cp\u003eYes — we offer custom assays. Customization can include target, pack size, reaction volume, master mix format, and inclusion of an internal control. Pricing is evaluated based on your specific customization requirements and quoted accordingly. For details, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/details\u003e\n\u003cdetails class=\"josh-faq-details\"\u003e\n\u003csummary class=\"josh-faq-summary\"\u003eWill your kits work with any PCR system? \u003cspan class=\"josh-faq-icon\"\u003e\u003c\/span\u003e\u003c\/summary\u003e\n\u003cdiv class=\"josh-faq-content\"\u003e\n\u003cp\u003eOur kits use standard probe-based fluorescence chemistry and are compatible with virtually all real-time PCR platforms. They are validated and optimized on widely used systems — including the Applied Biosystems QuantStudio series — and run reliably on other mainstream instruments using compatible detection channels. If you let us know your specific instrument model, we are happy to confirm the optimal channel and filter settings for your setup.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/details\u003e\n\u003cdetails class=\"josh-faq-details\"\u003e\n\u003csummary class=\"josh-faq-summary\"\u003eDo you offer discounts for bulk orders of kits? \u003cspan class=\"josh-faq-icon\"\u003e\u003c\/span\u003e\u003c\/summary\u003e\n\u003cdiv class=\"josh-faq-content\"\u003e\n\u003cp\u003eYes — we offer substantial discounts for bulk orders on most PCR kits. Discounts are available upon request through quotes only and do not apply to online orders. Bulk discounts apply to kits of the same type ordered at the same time, following this standard structure:\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e5% OFF (Qty. 5–9 kits)\u003c\/li\u003e\n\u003cli\u003e10% OFF (Qty. 10–14 kits)\u003c\/li\u003e\n\u003cli\u003e25% OFF (Qty. 15–29 kits)\u003c\/li\u003e\n\u003cli\u003e35% OFF (Qty. 30+ kits)\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eFor more information about discounted pricing, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/details\u003e\n\u003cdetails class=\"josh-faq-details\"\u003e\n\u003csummary class=\"josh-faq-summary\"\u003eDo you ship qPCR kits internationally? \u003cspan class=\"josh-faq-icon\"\u003e\u003c\/span\u003e\u003c\/summary\u003e\n\u003cdiv class=\"josh-faq-content\"\u003e\n\u003cp\u003eYes — we ship internationally via FedEx International Express (generally CIF or DAP terms). All international shipments include dry ice sufficient for maintaining low-temperature conditions during transit. We can also assist with customs clearance by providing required documentation such as COAs, MSDS, and HS codes. Dry ice surcharges may apply.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/details\u003e\n\u003cdetails class=\"josh-faq-details\"\u003e\n\u003csummary class=\"josh-faq-summary\"\u003eDo you ship qPCR kits on ice packs or at room temperature? \u003cspan class=\"josh-faq-icon\"\u003e\u003c\/span\u003e\u003c\/summary\u003e\n\u003cdiv class=\"josh-faq-content\"\u003e\n\u003cp\u003eNo. We do not ship qPCR kits on blue ice or at room temperature due to our strict quality requirements. This kit is shipped under controlled cold-chain conditions. Kits should be stored at −20°C for best performance.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/details\u003e\n\u003cdetails class=\"josh-faq-details\"\u003e\n\u003csummary class=\"josh-faq-summary\"\u003eAre your kits approved for diagnostic or clinical use? \u003cspan class=\"josh-faq-icon\"\u003e\u003c\/span\u003e\u003c\/summary\u003e\n\u003cdiv class=\"josh-faq-content\"\u003e\n\u003cp\u003eNo. All Biofargo kits are strictly for research use only (RUO). Biofargo, Inc. is not authorized to sell products for diagnostic, detection, clinical, personal, or any other non-research application. Use of our kits outside of an authorized laboratory research context is not permitted.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/details\u003e\n\u003c\/div\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/BF-452003-Filovirus_Type_Probe_qRT-PCR_Kit.pdf?v=1784793654\" rel=\"noopener\" target=\"_blank\"\u003e\u003cstrong\u003eFilovirus Type Probe qRT-PCR Kit User Manual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"PLEX","offers":[{"title":"Default Title","offer_id":52812543131829,"sku":"BF-452003","price":0.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/1_9cbc04eb-c58d-47b8-863f-8c2ad8f4aa0d.jpg?v=1784800372","url":"https:\/\/biofargo.com\/products\/filovirus-type-rt-pcr-kit-2-0-ruo-ebola-marburgvirus-rna-detection","provider":"Biofargo","version":"1.0","type":"link"}