Description

Ficoll 400: Ficoll 400 is a highly branched polymer formed by the copolymerization of sucrose and epichlorohydrin. Ficoll 400 is completely non-ionic. Because of the abundance of hydroxyl groups, Ficoll 400 is very hydrophilic and extremely water-soluble. The most common application for Ficoll 400 is as a density gradient medium for the separation and isolation of eukaryotic cells, organelles and bacterial cells. Density ranges up to 1.2 g/ml can be attained. It has also been utilized in a variety of other applications.

Specifications

Shipping Conditions	RT
UNSPSC Code	41116113
UNSPSC Category	Cell Separation Products
Grade	ACS
CAS	[26873-85-8]
DG	No
Storage	18 to 25℃
Sterile	Yes

Application

Suitable for

hybridization experiments to reduce non-specific binding of labeled probes to the membrane
preparation of DNA loading buffer (15% Ficoll®, 0.25% bromophenol blue and 0.25% xylene cyanol FF
separation of cells with similar density but different size (PBMC from human peripheral blood, bone marrow and umbilical cord blood)
producing density gradients to separate subcellular components
vitrification of cell and tissue samples.
to compare the effect of different perfusates on Hypoxic pulmonary vasoconstriction (HPV).

Used for cell separation and organelle isolation.

Features

free from DNases, RNases, phosphatases and proteases
preserves functional and morphological integrity of the separated cells

Documents

COA

SDS

Disclaimer: For laboratory research use only.