Description

Heat Inactivated FBS is ideal for the most sensitive cell lines and can be used to culture all cell types including macrophages, cancer and cancer related cells, Hybridomas and more. It works great for all our human primary cells and stem cells. Our FBS is manufactured in a fully integrated environment from raw material collection to finished product. It is produced to the highest standards in order to reduce risk and product variability, and to ensure product safety and performance.

Triple 0.1µm sterile-filtered
Mycoplasma-tested and virus-screened
Ships on dry ice. Store frozen at -20 to -10°C

Frequently Asked Questions

What is heat inactivated fetal bovine serum (FBS)?

Heat inactivated fetal bovine serum (FBS) is serum that has been treated at 56°C for 30 minutes to reduce complement activity. Heat inactivation is commonly used for sensitive cell culture applications, including macrophage culture, hybridoma production, stem cell research, and immunological studies.

Why use heat inactivated FBS instead of regular FBS?

Heat inactivated FBS helps minimize complement-mediated effects that may interfere with cell growth or experimental outcomes. Many researchers choose heat inactivated serum for hybridoma culture, primary cells, cancer cell lines, stem cells, and immune cell studies where consistent culture conditions are important.

What is the difference between heat inactivated FBS and standard FBS?

The primary difference is that heat inactivated FBS undergoes controlled heating to reduce complement activity. Standard FBS retains active complement proteins. Heat inactivated FBS is often preferred for sensitive mammalian cell culture and immunology-related applications.

What does USDA-origin FBS mean?

USDA-origin FBS is collected and processed in accordance with United States Department of Agriculture requirements. USDA-origin serum provides traceability and quality assurance that are important for academic, biotechnology, and pharmaceutical research laboratories.

Is this FBS suitable for stem cell culture?

Yes. Lonsera Heat Inactivated USDA-Origin FBS is suitable for many stem cell culture workflows, including human primary cells, mesenchymal stem cells (MSCs), and other sensitive cell types that benefit from reduced complement activity and consistent serum performance.

Can this heat inactivated FBS be used for hybridoma culture?

Yes. Hybridoma culture is one of the most common applications for heat inactivated fetal bovine serum. Reduced complement activity may help support stable cell growth and monoclonal antibody production.

Is this FBS suitable for cancer cell culture?

Yes. Heat inactivated FBS is widely used in cancer research, tumor biology studies, drug screening, and oncology-related cell culture applications. It supports the growth of many adherent and suspension cancer cell lines.

Has this serum been tested for mycoplasma contamination?

Yes. Lonsera Heat Inactivated FBS is mycoplasma-tested and quality controlled to help ensure reliable cell culture performance and reduce the risk of contamination.

What does triple 0.1 μm sterile filtration mean?

Triple 0.1 μm sterile filtration refers to three sequential filtration steps through 0.1 μm filters. This process helps improve sterility assurance while maintaining the biological performance of the serum for cell culture applications.

Is this FBS virus-screened?

Yes. The serum undergoes virus screening as part of the quality control process to support product safety, consistency, and suitability for research use.

Which cell types can be cultured with this heat inactivated FBS?

This serum can be used for a broad range of mammalian cell culture applications, including primary cells, macrophages, fibroblasts, hybridomas, stem cells, HEK293 cells, CHO cells, cancer cell lines, and other research cell models.

How should heat inactivated FBS be stored?

Heat inactivated FBS should be stored frozen at -20°C to -10°C. For long-term storage, avoid repeated freeze-thaw cycles, as they may affect serum performance and consistency.

Why is lot-to-lot consistency important when choosing FBS?

Lot-to-lot consistency helps ensure reproducible cell growth, experimental reliability, and reduced variability between studies. Consistent serum quality is particularly important for stem cell culture, biologics development, and long-term cell-based research projects.

Please note: For U.S. Customers, all serum is of USDA origin.

Fetal Bovine Serum (FBS)-Heat Inactivated USDA - Lonsera