Description

The FeaVacu® Vacuum Filtration Device with 0.22 μm hydrophilic PTFE membrane is designed for high-throughput filtration of aqueous solutions, buffers, cell culture media, and chemically demanding reagents. This disposable vacuum filtration system supports reliable bacterial-retentive filtration, clean filtrate, and broad chemical compatibility, making it ideal for laboratory, research, bioprocess, and analytical applications.

Important Note: This device is non-sterile as supplied. If sterile conditions are required, the device must be autoclaved prior to use. Hydrophilic PTFE membranes cannot be sterilized by gamma irradiation or chemical sterilization and should only be sterilized by autoclaving.

Constructed from USP Class VI polystyrene, the device is available in 250 mL, 500 mL, and 1000 mL formats, featuring a vacuum bottle top with nozzle and a receiving bottle. Its single-use design minimizes cross-contamination risk and simplifies laboratory workflows. After autoclaving of cell culture media, buffer preparation, and analytical sample processing.

Specifications

Parameter	Specification
Product Name	Vacuum Filtration Device with Hydrophilic PTFE Membrane
Product Type	Vacuum Filtration Device
Available Specifications	250 mL, 500 mL, 1000 mL
Pore Size	0.22 μm
Membrane Material	Hydrophilic PTFE
Raw Material	Polystyrene (PS)
Construction	Vacuum bottle top with nozzle and receiving bottle
Sterilization	Non-Sterile as supplied; autoclave before aseptic use
Bacterial Challenge	>10⁷ cfu/cm² B. diminuta (ATCC 19146)
Biosafety	Component materials meet the criteria of USP <88> Biological Reactivity for Class VI
Cleanness	Complies with FDA 21 CFR 211.72 and 210.3(b)(5)(6) for non-fiber-releasing filters, and USP <788> for insoluble particulate levels in flush water
Material Toxicity	Component materials meet USP <88> Class VI plastics biological reactivity standards
Endotoxin Level	<0.25 EU/mL USP bacterial endotoxins; LAL test, sample aqueous extraction
Manufacturing Environment	Manufactured in a 100,000 class clean room

Ordering Information

SKU	Membrane	Pore Size	Volume	Sterilization	Package
HD-02-FV-25-R-12	Hydrophilic PTFE	0.22 μm	250 mL	Non-Sterile	12 units/pack
HD-02-FV-50-R-12	Hydrophilic PTFE	0.22 μm	500 mL	Non-Sterile	12 units/pack
HD-02-FV-100-R-12	Hydrophilic PTFE	0.22 μm	1000 mL	Non-Sterile	12 units/pack

Features

Hydrophilic PTFE Membrane — Excellent for aqueous, solvent-sensitive, and chemically demanding filtration workflows.
0.22 μm Filtration — Efficiently removes bacteria and particulate contaminants.
Broad Chemical Compatibility — Supports buffers, culture media, and analytical reagents.
High Throughput & Flow Rate — Vacuum-driven format for rapid processing of large sample volumes.
Low Adsorption — Preserves active compounds and reduces sample loss.
Single-Use Disposable Design — Reduces cross-contamination risk and supports aseptic workflows after autoclaving.
Multiple Volume Options — 250 mL, 500 mL, 1000 mL for flexible workflows.
USP Class VI Polystyrene Construction — Ensures biocompatibility and regulatory compliance.

Applications

Buffer Filtration — Filtration and clarification of laboratory buffers.
Reagent Filtration — Filtration of aqueous and chemically sensitive solutions.
Cell Culture Media — Filtration of mammalian or microbial culture media. For sterile workflows, autoclave the device before use.
Heat-Sensitive Solutions — For solutions unsuitable for autoclaving.
Analytical Sample Preparation — High-purity filtrate for HPLC, spectrometry, or PCR workflows.
Research & Laboratory — Bioprocessing, pharmaceutical R&D, and general laboratory filtration.

Frequently Asked Questions (FAQ)

Is the FeaVacu® Vacuum Filtration Device sterile?

No. The FeaVacu® device is non-sterile by default. If sterile filtration is required, the device must be autoclaved prior to use. Note that the hydrophilic PTFE membrane cannot be sterilized by gamma irradiation or chemical sterilization and should only be sterilized using autoclaving methods.

What is the membrane pore size of FeaVacu® Vacuum Filtration Device?

The device features a 0.22 μm hydrophilic PTFE membrane, suitable for bacterial retention and sterile filtration.

Can this non-sterile vacuum filtration device be used for sterile filtration?

Yes. The FeaVacu® Vacuum Filtration Device can be used for sterile filtration workflows after appropriate steam sterilization. The device is supplied non-sterile and should be autoclaved prior to use when aseptic processing is required. Hydrophilic PTFE membranes are not suitable for gamma irradiation sterilization.

Is this device compatible with chemically demanding solutions?

Yes, the hydrophilic PTFE membrane supports a broad range of aqueous solutions and reagents including buffers, media, and solvents compatible with PTFE.

Can I use it for cell culture media filtration?

Absolutely. FeaVacu® is ideal for sterile filtration of mammalian or microbial cell culture media before inoculation.

What volume options are available?

Available volumes include 250 mL, 500 mL, and 1000 mL formats to suit various laboratory workflows.

Is the device single-use or reusable?

The FeaVacu® device is single-use, disposable, and reduces cross-contamination risks.

How does FeaVacu® compare to vacuum filtration devices from leading brands?

The FeaVacu® Vacuum Filtration Device with hydrophilic 0.22 μm PTFE membrane offers performance and features comparable to vacuum filtration devices from established brands such as Pall® (AcroPak™), Millipore® (Steriflip™, Express™), and Sartorius® (Sartorius® vacuum units). Like these leading devices, FeaVacu® provides reliable bacterial retention, broad chemical compatibility, and flexible volume options (250 – 1000 mL), making it suitable for laboratory, cell culture media filtration, sterile buffer preparation, and analytical workflows.

FeaVacu® Vacuum Filtration Device – Hydrophilic PTFE Membrane – 0.22 μm – 250 / 500 / 1000 mL