Specification

Full name	EYFP tag mouse monoclonal antibody
Alternative names	50 μl/100 μl
Reactivity	mouse monoclonal
Applications	WB
Host	Mouse
Clone type	mouse monoclonal
Target Background	Yellow Fluorescent Protein (YFP) is a genetic mutant of green fluorescent protein, derived from Aequorea victoria. Its excitation peak is 514nm and its emission peak is 527nm. Like green fluorescent protein (GFP), it is a useful tool in cell and molecular biology, usually explored using fluorescence microscopy. Three improved versions of YFP are Citrine, Venus, and Ypet. They have reduced chloride sensitivity, faster maturation, and increased brightness (product of the extinction coefficient and quantum yield). Typically, yellow FPs serve as the acceptor for genetically-encoded FRET sensors of which the most likely donor FP is mCFP (monomeric cyan FP). The red-shift relative to GFP is caused by a Pi-Pi stacking interaction as a result of the T203Y mutation, which essentially increases the polarizability of the local chromophore environment as well as providing additional electron density into the chromophore.
Swissprot No	nan
Gene Accession	nan
WB Predicted band size	27 kDa
WB Positive control
WB Recommended dilution	5000-20000
IHC predicted cell location	nan
IHC positive control	nan
IHC Recommed dilution	nan

Storage

Ascitic fluid with 0.05% sodium azide and 40% glycerol

P114236-WB-1-P