Properties

conjugate	peroxidase conjugate
Quality Level	200
form	buffered aqueous solution
technique(s)	direct ELISA: 1:2,000 dot blot: 1:4,000 immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100
shipped in	wet ice
storage temp.	-20˚C

Specificity

The product is found to be reactive with Anti-Avidin, Catalogue Number A5170, and non-reactive with Anti-Streptavidin, Catalogue Number S6390, by immunoelectrophoresis.

Application

ExtrAvidin®-FITC is suitable for the following applications:

immunohistochemistry (formalin-fixed, paraffin-embedded sections) at a concentration of 20μg/mL using human tissues.

Immunofluorescence

competitive binding assay

It has also been used for:

Immunoprecipitation and Immunobloting
Ra2I solid-phase binding assays
Southwestern blotting
Western blotting
Immunocytochemistry

Biochem/physiol Actions

Avidin is a tetrameric or dimeric biotin-binding protein produced in the oviducts of birds, reptiles and amphibians and deposited in the whites of their eggs. It consists of four high affinity binding sites for biotin. ExtrAvidin^® is prepared from egg white avidin. It is a modified form of affinity purified avidin with high specific activity of avidin and low background staining of streptavidin. It is a biotin binding protein produced by the bacteria Streptomyces avidinii.ExtrAvidin-Peroxidase may be used in conjunction with biotin in different techniques.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 0.05% MIT

Preparation Note

Affinity purified protein

Storage and Stability

At -20˚C for one year from date of receipt. Avoid repeated freezing and thawing. Protected from light. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

Legal Information

ExtrAvidin is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

ExtrAvidin®-FITC-Biofargo