Description

The Master Mix is used for real-time qualitative and quantitative RT-PCR amplifications with SYBR Green dye for extraction-free RNA samples. The master mix is a premixed, 2X concentrated solution that has all the components except for gene-specific primers and RNA template

Kit Characteristics

The master mix contains extra more redundant amounts of the RTase and DNA polymerase, specifically designed to overcome endogenous inhibition in extraction-free RNA samples.
For the reverse transcription step, this kit uses a highly efficient Thermophilic Reverse Transcriptase (US patent pending), which is a thermophilic type A polymerase, with optimal temperatures of 60-62°C.
The RTase is easily heat-inactivated at ≥90°C for 1min.
The RTase efficiently synthesizes a complementary DNA strand on RNA template from a gene-specific primer, ≤1 unit per 20μL of reaction.
The RTase reversely-transcribes single digit copies of target RNA molecules consistently.
The kit also contains Taq-Fast DNA polymerase which extends more than 300 bases with short cycling program.
The concentrations of the primers are variable depending on assay designs and thermocycling protocols (Table 1).
The preferable PCR product size is ≤150bp.
The kit has three formulations of No ROX, Low ROX or High ROX concentrations for your choice.

Components

2X Master Mix (2x1mL/5x1mL/10mL for 200/500/1000 reactions x 20μL) and an instruction for use.
The kit also contains Lysis Buffer (2x10mL/1x50mL/1x100mL).

Storage

Store at -20°C and transport at ≤4°C (up to three days).

Product Manual

User Manual

Extraction-Free RT-PCR Master Mix, SYBR Green Dye (#142)