Description

Escherichia coli Total RNA Residual Probe Method qRT-PCR Kit is designed for the quantitative detection of its specific DNA in a real-time PCR test using hydrolysis probes. In the amplification process, Taq DNA polymerase amplifies the DNA sample via quantitative PCR using a primer-probe set that targets its specific DNA region. At each cycle of the PCR, the 5' nuclease activity of Taq DNA polymerase degrades the bound probes, releasing the quenched specific FAM signal. The increase in FAM fluorescence measures the target gene-specific DNA amplifications. At a point where the fluorescence signal is confidently detected over the background signal, a Ct value can be determined for the FAM signal. This Ct parameter can be used to evaluate relative target abundance among samples.

Key Features

One-tube qPCR format minimizes carry-over contamination risk

High sensitivity: analytical LOD as low as 100 copies/reaction
Excellent specificity: no cross-reactivity with DNA from other pathogens
Includes amplification positive control for result verification
Instrument compatible: ABI, Bio-Rad, Roche, QuantStudio, etc.
Suitable for both qualitative and quantitative applications
For quantitative use: the linear range covers a 100,000-fold concentration difference.
Sufficient for 50 reactions (20 μL each)
For Research Use Only (RUO)

Kit Components

Component Name	Product Number.	Size	Vial	Lid Color
2×Probe qPCR MasterMix	981201	0.5 mL	0.5 mL Vial	Natural
Template Dilution Buffer	180701	1 mL	1.5 mL Vial	Green
Primer-Probe Powder	yp15-175R	50 T	0.5 mL Brown Vial	Brown
Amplification Positive Control (1E7 copies/μL)	pc175R	50 μL	0.5 mL Vial	Yellow
Ultrapure water	210806	1 mL	1.5 mL Vial	Blue
Manual	sc15-175R	1 Set	NA	NA

Application Areas

Seafood Processing & Quality Control: Rapid qRT-PCR–based screening of Escherichia coli residual RNA in shellfish and marine products to ensure compliance with HACCP, FDA/EFSA regulations and minimize product recalls.
Aquaculture Health Monitoring: High-sensitivity detection of E. coli total RNA in abalone, shrimp and fish farms for real-time disease surveillance, pathogen risk assessment and optimized farm management.
Marine Environmental Surveillance: Quantitative tracking of E. coli residual RNA in seawater, sediment and biofilms to support ecological risk assessments, water quality monitoring and coastal ecosystem protection.

Storage & Shipping

Store at - 20 °C
The product is shipped with dry ice to maintain low-temperature conditions and ensure product integrity during transit.

Documents

Escherichia coli Total RNA Residual Probe Method qRT-PCR Kit User Manual

MSDS