Description

Escherichia coli O157/Listeria monocytogenes Duplex Probe qPCR Kit is designed for the quantitative detection of its specific DNA in a real-time PCR test using hydrolysis probes. In the amplification process, Taq DNA polymerase amplifies the DNA sample via quantitative PCR using a primer-probe set that targets its specific DNA region. At each cycle of the PCR, the 5' nuclease activity of Taq DNA polymerase degrades the bound probes, releasing the quenched specific FAM signal. The increase in FAM fluorescence measures the target gene-specific DNA amplifications. At a point where the fluorescence signal is confidently detected over the background signal, a Ct value can be determined for the FAM signal. This Ct parameter can be used to evaluate relative target abundance among samples.

Key Features

One-tube qPCR format minimizes carry-over contamination risk

High sensitivity: analytical LOD as low as 100 copies/reaction
Excellent specificity: no cross-reactivity with DNA from other pathogens
Includes amplification positive control for result verification
Compatible with various DNA purification methods
Suitable for both qualitative and quantitative applications
For quantitative use: the linear range covers a 100,000-fold concentration difference.
Sufficient for 50 reactions (20 μL each)
For Research Use Only (RUO)

Kit Components

Component Name	Product Number.	Size	Vial	Lid Color
2×Probe qPCR MasterMix	981201	0.5 mL	0.5 mL Vial	Natural
Template Dilution Buffer	180701	1 mL	1.5 mL Vial	Green
Primer-Probe Powder	yp15-14873	50 T	0.5 mL Brown Vial	Brown
Amplification Positive Control (1E7 copies/μL)	pc14873	50 μL	0.5 mL Vial	Yellow
Ultrapure water	210806	1 mL	1.5 mL Vial	Blue
Manual	sc15-14873	1 Set	NA	NA

Application Areas

Food safety testing: quantitative detection of Escherichia coli O157 and Listeria monocytogenes in a wide range of food matrices (e.g., meat, dairy, fresh produce, ready‑to‑eat products) and in enrichment cultures to support contamination screening and release decisions.
Food processing and HACCP quality control: rapid in‑plant screening of product lots, environmental swabs (surfaces, equipment), and process water to monitor sanitation effectiveness and verify critical control points.
Research and method development (RUO): quantitative surveillance, assay validation, spiking/recovery studies, and microbiological research in laboratories developing or optimizing detection workflows for E. coli O157 and L. monocytogenes.

Storage & Shipping

Store at - 20 °C
The product is shipped with dry ice to maintain low-temperature conditions and ensure product integrity during transit.

FAQ

Q: Are Biofargo PCR and qPCR Kits compatible with my thermal cycler or real-time PCR instrument?

A: Yes. Biofargo PCR Kits and probe-based qPCR Kits are designed for broad compatibility with mainstream instruments.

PCR Kits can be used with standard thermal cyclers from leading brands.

Probe-based qPCR Kits are fully compatible with major real-time PCR platforms, including ABI, Bio-Rad, Roche, QuantStudio, Qiagen Rotor-Gene Q (5-plex), and Genesig Q16.

Our technical team is available to assist with instrument setup and run optimization to ensure the best results.

Q: Can this kit be used for digital droplet PCR (ddPCR)?

A: Yes.

Documents

Escherichia coli O157/Listeria monocytogenes Duplex Probe qPCR Kit User Manual

MSDS