Description

Fragmentation of nucleic acids, chromatin, or proteins for NGS

The EoSonics® FRAG96 Sonicator uses ACU™ (Adaptive Cylindrical Ultrasonic) technology to process extracted nucleic acids, chromatin, or proteins. By adjusting acoustic parameters, it precisely controls the fragmentation length of nucleic acids.

EoSonics® FRAG96 can process 1 to 96 samples per batch and is compatible with a wide range of consumables, including 96-well PCR plates, cell culture plates, and 8-strip tubes. This flexibility minimizes consumable costs when running different sample throughputs. The system eliminates the need to manually transfer samples to dedicated consumables, enabling whole-plate transfer for seamless automation.

Compared to enzymatic methods, physical ultrasonic shearing offers significant advantages, such as non-preferential sequence cutting and a narrower fragment size distribution, making it the preferred choice for sample fragmentation. In addition, the FRAG96 does not require specialized consumables, which further reduces operating costs.

Features

High Throughput: Processes up to 96 samples per batch.
Low Noise: Operates at ultra-high frequencies without vibration or disruptive noise.
High Flexibility: Supports flexible sample and consumable throughput from 1 to 96 samples.
No Plate Transfer: Enables direct processing of nucleic extracts and cell cultures without plate transfer.
Non-contact Processing: Fully enclosed reaction system prevents sample contamination.
Broad Compatibility: Works with standard laboratory consumables—no specialized plastics required.
Edge Effect Elimination: Ensures highly consistent fragmentation across all wells.
Fatigue Resistant: Designed for long-term continuous operation.

Applications

The FRAG96 Sonicator is suitable for a wide range of laboratory applications, including:

DNA/RNA/chromatin shearing
Nucleic acid and protein extraction
Cell lysis
Tissue fragmentation
Dissolution of complex mixtures
Protein digestion reactions

Based on results from pan-cancer DNA library preparation and NGS sequencing, the sample fragmentation capability of the LivoBot Focused-ultrasonicator has been demonstrated to be fully compatible with the Illumina TSO500 panel kit.

The image demonstrates the DNA fragmentation result using commonly used 8-strip tube. The fragment sizes are all stable at 150～200bp.

EoSonics® Frag96 Sonicator