Description

Enterovirus 75 (EV75)Probe Realtime RT-PCR Kit is a one-step RT-PCR kit intended for research use in nucleic acid analysis under controlled laboratory conditions using probe-based real-time PCR. Purified RNA is reverse transcribed into cDNA and subsequently amplified using sequence-specific primers and probes. During amplification, fluorescence signals are generated through probe cleavage, allowing real-time monitoring of amplification under controlled laboratory conditions. The resulting signals may be used for comparative analysis of target nucleic acids.

Note: Supplied without internal control. Internal control version can be customized based on your assay requirements.
This product was previously listed under catalog number 15-15579 and is now updated to BF-11501006 for improved product management.

Key Features

One-step RT-PCR workflow to reduce handling steps and minimize contamination risk.
Demonstrates high sensitivity under optimized laboratory conditions.
High specificity based on primer-probe design.
Includes a non-infectious plasmid-based positive control for assay verification.
Compatible with commonly used RNA purification methods.
Suitable for research applications including assay development and method evaluation.
Broad dynamic range under laboratory conditions.
For research use only. Not intended for diagnostic or therapeutic use.

Kit Components

Component Name	Description	Size	Vial	Lid Color
2× One-Step RT-PCR Master Mix	Contains optimized buffer and enzyme mix	500 μL	0.5 μL Vial	Blue
Primer-Probe Mix	Lyophilized target-specific primer and probe set	50 T	1.5 mL Vial	Brown
Positive Control (1E7 copies/µL)	Non-infectious plasmid-based control	0.5 mL	0.5 mL Vial	Yellow
Nuclease-free water / Dilution Buffer	For reaction setup	1 mL	1.5 mL Vial	Green
Manual	Instruction booklet	1 Set	NA	NA

Storage & Shipping

Store at - 20 °C
The product is shipped with dry ice to maintain low-temperature conditions and ensure product integrity during transit.

Is a ROX Dye included with the kit?

No — ROX dye is not included with the kit. We can add a passive reference dye matched to your instrument upon request for $25 USD. To request a ROX dye, please contact us for a quote at contact@biofargo.com.

Is an Internal Control included with the kit?

Unless otherwise specified, internal controls are not included in our standard kits, but are available as an add-on for all kits at a surcharge of $150 USD. To request a kit with an internal control, please reach out for a quote at contact@biofargo.com.

Is validation data available for the kit?

Validation data may be available upon request, depending on the target. Standard validation generated from plasmid-based positive controls can typically be provided for many kits. Validation generated using the actual pathogen is handled case by case — it requires that we are able to source the pathogen, or that the customer supplies an extracted nucleic acid sample. Please note all kits are intended strictly for research use only. To enquire, contact us at contact@biofargo.com.

What is the lead time for all PCR kits?

Standard production takes 7–14 days, after which kits ship from our laboratory to our warehouse. Expedited production (~7 days) is available upon request. We also maintain a ready inventory of our most popular kits to reduce wait times.

Can I customize this kit?

Yes — we offer custom assays. Customization can include target, pack size, reaction volume, master mix format, and inclusion of an internal control. Pricing is evaluated based on your specific customization requirements and quoted accordingly. For details, please contact us at contact@biofargo.com.

Will your kits work with any PCR system?

Our kits use standard FAM-based probe chemistry and are compatible with virtually all real-time PCR platforms. They are validated and optimized on widely used systems — including the Applied Biosystems QuantStudio series — and run reliably on other mainstream instruments using the same FAM detection channel. If you let us know your specific instrument model, we are happy to confirm the optimal channel and filter settings for your setup.

Do you offer discounts for bulk orders of kits?

Yes — we offer substantial discounts for bulk orders on most PCR kits. Discounts are available upon request through quotes only and do not apply to online orders. Bulk discounts apply to kits of the same type ordered at the same time, following this standard structure:

5% OFF (Qty. 5–9 kits)
10% OFF (Qty. 10–14 kits)
25% OFF (Qty. 15–29 kits)
35% OFF (Qty. 30+ kits)

For more information about discounted pricing, please contact us at contact@biofargo.com.

Do you ship qPCR kits internationally?

Yes — we ship internationally via FedEx International Express (generally CIF or DAP terms). All international shipments include ~40 lbs of dry ice, sufficient for a maximum of 7 days in transit. We can also assist with customs clearance (completing paperwork and providing COAs, MSDS, HS codes, etc.). A dry ice surcharge applies: $15 USD for domestic shipments and $55 USD for international shipments.

Do you ship qPCR kits on ice packs or at room temperature?

No. We do not ship qPCR kits on blue ice or at room temperature due to our strict quality requirements. This kit is shipped on dry ice. Kits may retain limited stability at room temperature, but should be stored at −20°C for best performance.

Are your kits approved for diagnostic or clinical use?

No. All Biofargo kits are strictly for research use only (RUO). Biofargo, Inc. is not authorized to sell products for diagnostic, detection, clinical, personal, or any other non-research application. Use of our kits outside of an authorized laboratory research context is not permitted.

Documents

Enterovirus 75 (EV75)Probe Realtime RT-PCR Kit User Manual

MSDS