{"product_id":"endofree-maxi-plasmid-kit-v2","title":"EndoFree Maxi Plasmid Kit V2","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eThe EndoFree Maxi Plasmid Kit V2 adopts a unique silica membrane adsorption technique to efficiently and specifically bind plasmid DNA. The special Buffer ER and the Filtration CS1 can effectively remove impurities such as endotoxin and protein. The entire extraction process takes only 1 hour.\u003c\/p\u003e\n\u003cp\u003eRecommended bacteria culture medium amount: For high-copy plasmid, 100 ml bacteria medium is recommended, which can yield up to 500-1500 μg plasmid. For low-copy plasmid, 200 ml of bacteria medium is recommended to achieve the yield of around 50-300 μg.\u003c\/p\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003eHigh purity: The unique endotoxin precipitation technology is adopted to specifically remove endotoxin.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eEasy to operate: The adsorption column technology is used to specifically adsorb plasmid DNA, which makes the operation easier.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eHigh-efficiency transfection: Suitable for the transfection of most cell lines including endotoxin-sensitive cells.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eWide range of applications: The purified plasmid can be applied in the transfection of animal and plant cells as well as molecular biology experiments.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cp\u003eThe plasmid DNA extracted using this kit can be used for various regular operations, including restriction enzyme digestion, PCR, sequencing, ligation, transformation and transfection of various cells.\u003c\/p\u003e\n\u003ch2\u003eExperimental Procedure\u003c\/h2\u003e\n\u003cdiv style=\"text-align: start;\"\u003e\u003cimg style=\"float: none;\" alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/1_627c665e-6393-4349-9e6c-b5ca749002d7_600x600.jpg?v=1743587163\"\u003e\u003c\/div\u003e\n\u003ch2\u003eRequired Reagents\u003c\/h2\u003e\n\u003cp\u003eEthanol, isopropanol\u003c\/p\u003e\n\u003ch2\u003eStorage\u003c\/h2\u003e\n\u003cp\u003eRoom temperature (15-25℃ )\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca type=\"application\/pdf\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/20240712135455_22349.pdf?v=1743587238\" target=\"_blank\"\u003e\u003cb\u003eGDP120-EndoFree Maxi Plasmid Kit V2-240514\u003c\/b\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"Tiangen","offers":[{"title":"10 preps","offer_id":51301591122101,"sku":"GDP120-01","price":264.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/EndoFree_Maxi_Plasmid_Kit_V2.jpg?v=1752044732","url":"https:\/\/biofargo.com\/products\/endofree-maxi-plasmid-kit-v2","provider":"Biofargo","version":"1.0","type":"link"}