Description

This product is used for enzyme-linked immunosorbent assay termination of color reaction. Termination solution has two roles: First, the termination of the liquid destroys the activity of HRP enzyme, the catalytic function of the enzyme loss, stop the reaction, making the reading relatively stable and easy to control; the second is to stop the enzyme inactivation at the same time, make the pH decreased, The characteristic absorption wavelength of the chromogenic substrate changes, the blue cationic cation can be converted into yellow diphenoquinone, and the diphenoquinone has the maximum extinction coefficient at a wavelength of 450 nm.

Cat.No : E661006

Specifications

Parameter	Specification	Details
Product Name	ELISA termination solution	ELISA termination solution
Cat.No	E661006	E661006
Package	100 ml/200 ml	100 ml/200 ml
Storage	Store at Room temperature for 24 months	Store at Room temperature for 24 months
Component	1M H2SO4	1M H2SO4

Features

✔ Application: This product is used for enzyme-linked immunosorbent assay termination of color reaction.

✔ Function: The termination solution destroys HRP enzyme activity, stopping the reaction and stabilizing results.

✔ Signal Conversion: Converts blue substrate into yellow diphenoquinone with maximum absorbance at 450 nm.

✔ Component: Contains 1M H2SO4 for rapid and effective reaction termination.

Applications

ELISA Reaction Termination

Stops enzymatic color development in ELISA assays.

HRP Enzyme Inactivation

Rapidly inactivates HRP to stabilize signal output.

Signal Stabilization

Ensures consistent and reproducible absorbance readings.

450 nm Detection

Optimized for absorbance measurement at 450 nm.

Stability & Storage

Store at Room temperature for 24 months

Cautions

1. The product is corrosive, please use with proper protection.

2. For research use only. Not for clinical or diagnostic applications.

Documents

Download Data Sheet

ELISA Stopping Solution