Description

In routine molecular biology workflows, inconsistent DNA/RNA visualization and the safety risks associated with Ethidium Bromide (EtBr) continue to be a major concern in agarose gel electrophoresis and PAGE analysis. Researchers need a safer nucleic acid stain that maintains high sensitivity while reducing toxicity risks and simplifying gel imaging workflows.

Eco-Stain Ready-to-Use DNA/RNA Gel Stain is a next-generation Ethidium Bromide alternative designed for reliable detection of DNA, single-stranded DNA, and RNA in agarose and polyacrylamide gels. It delivers strong performance in gel electrophoresis DNA visualization while significantly improving laboratory safety and compliance in research environments.

Unlike traditional mutagenic dyes, Eco-Stain is classified as non-carcinogenic and demonstrates significantly reduced mutagenicity in standard toxicological assays. It is widely used in DNA gel stain and RNA gel stain workflows where both performance and biosafety are required, making it ideal for modern molecular biology, genetic analysis, and teaching laboratories.

With dual fluorescence capability, Eco-Stain enables clearer differentiation between nucleic acids—providing green fluorescence for DNA and red fluorescence for RNA—supporting more accurate interpretation in mixed-sample analysis and quality control workflows.

Specification

Product Name	Eco-Stain Ready-to-Use DNA/RNA Gel Stain
CAS No.	163795-75-3
UNSPSC Code	12171500
Category	Electrophoresis Tracking / Nucleic Acid Stain
Type	Fluorescent DNA/RNA Gel Stain
Storage	2–8°C
Shipping	Ice (ICE)
Safety Classification	Not Hazardous (SDS)
Excitation	~490 nm
Emission	520 nm (DNA), 635 nm (RNA)

Features

Ethidium Bromide Alternative: Designed as a safer replacement for EtBr in DNA/RNA gel electrophoresis workflows.
Dual Fluorescence Detection: Green fluorescence for dsDNA/ssDNA and red fluorescence for RNA enables clear nucleic acid differentiation.
High Sensitivity DNA Gel Stain: Detects low nanogram-level DNA bands in routine agarose gel analysis.
Broad Compatibility: Suitable for agarose gel electrophoresis and PAGE systems without major protocol changes.
Optimized Optical Performance: Excitation at ~490 nm with dual emission peaks (520/635 nm) for standard gel documentation systems.
Workflow Friendly: Ready-to-use formulation reduces preparation steps and minimizes contamination risk.
Biosafe Profile: Non-carcinogenic classification with significantly reduced mutagenicity in standard biological assays.

Applications

Agarose gel electrophoresis for PCR product verification and DNA fragment analysis
DNA restriction digestion and plasmid screening workflows
RNA gel electrophoresis for transcript quality and integrity assessment
PAGE (polyacrylamide gel electrophoresis) for high-resolution nucleic acid detection
RNA/DNA mixed sample analysis with dual-color fluorescence differentiation
Gel documentation systems using UV or blue light transilluminators
Teaching laboratories and biosafety-restricted environments as EtBr replacement dye

Safer Gel Electrophoresis Workflow

Eco-Stain supports modern gel electrophoresis DNA visualization workflows by eliminating the need for toxic EtBr handling in gel preparation. It integrates seamlessly into existing agarose gel and PAGE protocols, enabling laboratories to upgrade to a non-mutagenic nucleic acid staining system without changing standard experimental setups.

Documents

INFO SDS

Frequently Asked Questions

What is Eco-Stain used for?

Eco-Stain is used for DNA, RNA, and ssDNA visualization in agarose gel electrophoresis and PAGE systems as a safer Ethidium Bromide alternative.

Is Eco-Stain a safer alternative to Ethidium Bromide?

Yes. Eco-Stain is classified as non-carcinogenic and demonstrates significantly reduced mutagenicity compared to Ethidium Bromide in standard biological safety tests.

Can Eco-Stain detect both DNA and RNA?

Yes. Eco-Stain provides green fluorescence for DNA and red fluorescence for RNA, allowing clear differentiation in mixed nucleic acid samples.

What imaging systems are compatible with Eco-Stain?

Eco-Stain is compatible with standard UV transilluminators and gel documentation systems operating at ~490 nm excitation with dual emission detection.

How should Eco-Stain be stored?

Store at 2–8°C and protect from light to maintain optimal fluorescence performance.

For laboratory research use only.

Eco-Stain, ready to use (EtBr alternative)