{"product_id":"dsdna-hs-assay-kit-starlighter","title":"dsDNA HS Assay Kit——StarLighter","description":"\u003csection\u003e\n  \u003ch2\u003eDescription\u003c\/h2\u003e\n  \u003cp\u003eLow-concentration double-stranded DNA samples can be difficult to quantify using traditional UV A260 measurements. The StarLighter dsDNA HS Assay Kit provides a high-sensitivity, fluorescence-based method for selective quantification of double-stranded DNA. The assay detects initial dsDNA concentrations from 5 pg\/µL to 120 ng\/µL, depending on sample volume, with a total assay range of 0.1–120 ng.\u003c\/p\u003e\n\n  \u003cp\u003eThe kit contains a fluorescent reagent, assay buffer, and two dsDNA standards. It provides high selectivity for dsDNA over RNA and tolerates specified concentrations of common laboratory contaminants, including salts, free nucleotides, solvents, detergents, and proteins.\u003c\/p\u003e\n\n  \u003cp\u003eThe protocol supports two measurement formats. Samples can be measured individually using a Qubit fluorometer and 0.5 mL thin-walled clear analysis tubes, or processed in a black 96-well microplate using a fluorescence microplate reader. Each assay accepts 1–20 µL of sample in a final reaction volume of 200 µL.\u003c\/p\u003e\n\n  \u003cp\u003eThe working solution is prepared by diluting dsDNA HS Fluorescent Reagent QF into dsDNA HS Buffer QB at a 1:199 ratio. After mixing, the reaction requires a 2-minute incubation at room temperature while protected from light. The fluorescent signal remains stable for up to 3 hours after incubation when protected from light.\u003c\/p\u003e\n\n  \u003cp\u003eThe StarLighter dsDNA HS Assay Kit is available in 20-reaction and 500-reaction formats. This product is for research use only and is not intended for diagnostic or therapeutic procedures.\u003c\/p\u003e\n\u003c\/section\u003e\n\n\u003csection\u003e\n  \u003ch2\u003eSpecification\u003c\/h2\u003e\n\n  \u003cdiv style=\"width:100%; overflow-x:auto; -webkit-overflow-scrolling:touch;\"\u003e\n    \u003ctable style=\"width:100%; min-width:680px; border-collapse:collapse;\"\u003e\n      \u003ctbody\u003e\n        \u003ctr\u003e\n          \u003cth style=\"border:1px solid #d9d9d9; padding:10px; text-align:left;\"\u003eProduct Name\u003c\/th\u003e\n          \u003ctd style=\"border:1px solid #d9d9d9; padding:10px;\"\u003eStarLighter dsDNA HS Assay Kit\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003cth style=\"border:1px solid #d9d9d9; padding:10px; text-align:left;\"\u003eTarget\u003c\/th\u003e\n          \u003ctd style=\"border:1px solid #d9d9d9; padding:10px;\"\u003eDouble-stranded DNA (dsDNA)\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003cth style=\"border:1px solid #d9d9d9; padding:10px; text-align:left;\"\u003eDetection Method\u003c\/th\u003e\n          \u003ctd style=\"border:1px solid #d9d9d9; padding:10px;\"\u003eFluorescence-based quantification\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003cth style=\"border:1px solid #d9d9d9; padding:10px; text-align:left;\"\u003eInitial dsDNA Concentration Range\u003c\/th\u003e\n          \u003ctd style=\"border:1px solid #d9d9d9; padding:10px;\"\u003e5 pg\/µL–120 ng\/µL, depending on sample volume\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003cth style=\"border:1px solid #d9d9d9; padding:10px; text-align:left;\"\u003eTotal Assay Range\u003c\/th\u003e\n          \u003ctd style=\"border:1px solid #d9d9d9; padding:10px;\"\u003e0.1–120 ng\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003cth style=\"border:1px solid #d9d9d9; padding:10px; text-align:left;\"\u003eSample Volume\u003c\/th\u003e\n          \u003ctd style=\"border:1px solid #d9d9d9; padding:10px;\"\u003e1–20 µL\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003cth style=\"border:1px solid #d9d9d9; padding:10px; text-align:left;\"\u003eFinal Reaction Volume\u003c\/th\u003e\n          \u003ctd style=\"border:1px solid #d9d9d9; padding:10px;\"\u003e200 µL\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003cth style=\"border:1px solid #d9d9d9; padding:10px; text-align:left;\"\u003eWorking Solution Ratio\u003c\/th\u003e\n          \u003ctd style=\"border:1px solid #d9d9d9; padding:10px;\"\u003e1 part dsDNA HS Fluorescent Reagent QF to 199 parts dsDNA HS Buffer QB\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003cth style=\"border:1px solid #d9d9d9; padding:10px; text-align:left;\"\u003eStandards\u003c\/th\u003e\n          \u003ctd style=\"border:1px solid #d9d9d9; padding:10px;\"\u003eQS0: 0 ng\/µL; QS1: 10 ng\/µL\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003cth style=\"border:1px solid #d9d9d9; padding:10px; text-align:left;\"\u003eAssay Temperature\u003c\/th\u003e\n          \u003ctd style=\"border:1px solid #d9d9d9; padding:10px;\"\u003e20–25°C\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003cth style=\"border:1px solid #d9d9d9; padding:10px; text-align:left;\"\u003eIncubation Time\u003c\/th\u003e\n          \u003ctd style=\"border:1px solid #d9d9d9; padding:10px;\"\u003e2 minutes at room temperature, protected from light\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003cth style=\"border:1px solid #d9d9d9; padding:10px; text-align:left;\"\u003eSignal Stability\u003c\/th\u003e\n          \u003ctd style=\"border:1px solid #d9d9d9; padding:10px;\"\u003eUp to 3 hours after incubation when protected from light\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003cth style=\"border:1px solid #d9d9d9; padding:10px; text-align:left;\"\u003eCompatible Instruments\u003c\/th\u003e\n          \u003ctd style=\"border:1px solid #d9d9d9; padding:10px;\"\u003eQubit fluorometer or fluorescence microplate reader\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003cth style=\"border:1px solid #d9d9d9; padding:10px; text-align:left;\"\u003eMicroplate Reader Settings\u003c\/th\u003e\n          \u003ctd style=\"border:1px solid #d9d9d9; padding:10px;\"\u003eExcitation: 485 nm; Emission: 530 nm\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003cth style=\"border:1px solid #d9d9d9; padding:10px; text-align:left;\"\u003eShipping Condition\u003c\/th\u003e\n          \u003ctd style=\"border:1px solid #d9d9d9; padding:10px;\"\u003eShip on ice\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003cth style=\"border:1px solid #d9d9d9; padding:10px; text-align:left;\"\u003eStorage\u003c\/th\u003e\n          \u003ctd style=\"border:1px solid #d9d9d9; padding:10px;\"\u003e2–8°C upon arrival; protect fluorescent reagents from light\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003cth style=\"border:1px solid #d9d9d9; padding:10px; text-align:left;\"\u003eAvailable Sizes\u003c\/th\u003e\n          \u003ctd style=\"border:1px solid #d9d9d9; padding:10px;\"\u003e20 reactions and 500 reactions\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003cth style=\"border:1px solid #d9d9d9; padding:10px; text-align:left;\"\u003eIntended Use\u003c\/th\u003e\n          \u003ctd style=\"border:1px solid #d9d9d9; padding:10px;\"\u003eFor research use only\u003c\/td\u003e\n        \u003c\/tr\u003e\n      \u003c\/tbody\u003e\n    \u003c\/table\u003e\n  \u003c\/div\u003e\n\n  \u003ch3\u003eCatalog Numbers and Kit Components\u003c\/h3\u003e\n\n  \u003cdiv style=\"width:100%; overflow-x:auto; -webkit-overflow-scrolling:touch;\"\u003e\n    \u003ctable style=\"width:100%; min-width:720px; border-collapse:collapse;\"\u003e\n      \u003cthead\u003e\n        \u003ctr\u003e\n          \u003cth style=\"border:1px solid #d9d9d9; padding:10px; text-align:left;\"\u003eComponent\u003c\/th\u003e\n          \u003cth style=\"border:1px solid #d9d9d9; padding:10px; text-align:left;\"\u003eFS-T1001-S\u003cbr\u003e20 Reactions\u003c\/th\u003e\n          \u003cth style=\"border:1px solid #d9d9d9; padding:10px; text-align:left;\"\u003eFS-T1002\u003cbr\u003e500 Reactions\u003c\/th\u003e\n        \u003c\/tr\u003e\n      \u003c\/thead\u003e\n      \u003ctbody\u003e\n        \u003ctr\u003e\n          \u003ctd style=\"border:1px solid #d9d9d9; padding:10px;\"\u003edsDNA HS Buffer QB\u003c\/td\u003e\n          \u003ctd style=\"border:1px solid #d9d9d9; padding:10px;\"\u003e10 mL\u003c\/td\u003e\n          \u003ctd style=\"border:1px solid #d9d9d9; padding:10px;\"\u003e250 mL\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003ctd style=\"border:1px solid #d9d9d9; padding:10px;\"\u003edsDNA HS Standard QS0 (0 ng\/µL)\u003c\/td\u003e\n          \u003ctd style=\"border:1px solid #d9d9d9; padding:10px;\"\u003e200 µL\u003c\/td\u003e\n          \u003ctd style=\"border:1px solid #d9d9d9; padding:10px;\"\u003e5 mL\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003ctd style=\"border:1px solid #d9d9d9; padding:10px;\"\u003edsDNA HS Standard QS1 (10 ng\/µL)\u003c\/td\u003e\n          \u003ctd style=\"border:1px solid #d9d9d9; padding:10px;\"\u003e200 µL\u003c\/td\u003e\n          \u003ctd style=\"border:1px solid #d9d9d9; padding:10px;\"\u003e5 mL\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003ctd style=\"border:1px solid #d9d9d9; padding:10px;\"\u003edsDNA HS Fluorescent Reagent QF\u003c\/td\u003e\n          \u003ctd style=\"border:1px solid #d9d9d9; padding:10px;\"\u003e50 µL\u003c\/td\u003e\n          \u003ctd style=\"border:1px solid #d9d9d9; padding:10px;\"\u003e1.25 mL\u003c\/td\u003e\n        \u003c\/tr\u003e\n      \u003c\/tbody\u003e\n    \u003c\/table\u003e\n  \u003c\/div\u003e\n\n  \u003ch3\u003eRequired Materials Not Supplied\u003c\/h3\u003e\n  \u003cul\u003e\n    \u003cli\u003eFluorometer and 0.5 mL thin-walled clear analysis tubes, or a fluorescence microplate reader and black 96-well microplates\u003c\/li\u003e\n    \u003cli\u003eVortex mixer\u003c\/li\u003e\n    \u003cli\u003ePipettes and sterile, nuclease-free pipette tips\u003c\/li\u003e\n    \u003cli\u003eNuclease-free water or TE buffer for sample dilution, if required\u003c\/li\u003e\n  \u003c\/ul\u003e\n\u003c\/section\u003e\n\n\u003csection\u003e\n  \u003ch2\u003eFeatures\u003c\/h2\u003e\n  \u003cul\u003e\n    \u003cli\u003e\n\u003cstrong\u003eHigh-sensitivity dsDNA quantification:\u003c\/strong\u003e Detects initial dsDNA concentrations from 5 pg\/µL to 120 ng\/µL, depending on sample volume.\u003c\/li\u003e\n    \u003cli\u003e\n\u003cstrong\u003eDefined total assay range:\u003c\/strong\u003e Measures 0.1–120 ng of dsDNA per assay.\u003c\/li\u003e\n    \u003cli\u003e\n\u003cstrong\u003eSelective fluorescence measurement:\u003c\/strong\u003e Provides high selectivity for double-stranded DNA over RNA.\u003c\/li\u003e\n    \u003cli\u003e\n\u003cstrong\u003eLow sample requirement:\u003c\/strong\u003e Supports sample volumes from 1 to 20 µL.\u003c\/li\u003e\n    \u003cli\u003e\n\u003cstrong\u003eTwo measurement formats:\u003c\/strong\u003e The protocol can be performed using a Qubit fluorometer or a fluorescence microplate reader.\u003c\/li\u003e\n    \u003cli\u003e\n\u003cstrong\u003e96-well assay format:\u003c\/strong\u003e Supports fluorescence-based dsDNA quantification in black-wall, clear-bottom 96-well microplates.\u003c\/li\u003e\n    \u003cli\u003e\n\u003cstrong\u003eShort incubation:\u003c\/strong\u003e Requires a 2-minute incubation at room temperature while protected from light.\u003c\/li\u003e\n    \u003cli\u003e\n\u003cstrong\u003eStable post-incubation signal:\u003c\/strong\u003e The fluorescent signal remains stable for up to 3 hours when protected from light.\u003c\/li\u003e\n    \u003cli\u003e\n\u003cstrong\u003eTwo supplied standards:\u003c\/strong\u003e Includes QS0 at 0 ng\/µL and QS1 at 10 ng\/µL.\u003c\/li\u003e\n    \u003cli\u003e\n\u003cstrong\u003eTwo package sizes:\u003c\/strong\u003e Available as FS-T1001-S for 20 reactions and FS-T1002 for 500 reactions.\u003c\/li\u003e\n  \u003c\/ul\u003e\n\n  \u003ch3\u003eDocumented Contaminant Tolerance\u003c\/h3\u003e\n  \u003cp\u003eThe following table lists the contaminant concentrations evaluated in the assay. Results reported as “OK” usually produced less than 10% perturbation under the stated conditions.\u003c\/p\u003e\n\n  \u003cdiv style=\"width:100%; overflow-x:auto; -webkit-overflow-scrolling:touch;\"\u003e\n    \u003ctable style=\"width:100%; min-width:760px; border-collapse:collapse;\"\u003e\n      \u003cthead\u003e\n        \u003ctr\u003e\n          \u003cth style=\"border:1px solid #d9d9d9; padding:10px; text-align:left;\"\u003eContaminant\u003c\/th\u003e\n          \u003cth style=\"border:1px solid #d9d9d9; padding:10px; text-align:left;\"\u003eFinal Concentration in Assay\u003c\/th\u003e\n          \u003cth style=\"border:1px solid #d9d9d9; padding:10px; text-align:left;\"\u003eApproximate Concentration in 10 µL Sample\u003c\/th\u003e\n          \u003cth style=\"border:1px solid #d9d9d9; padding:10px; text-align:left;\"\u003eResult\u003c\/th\u003e\n        \u003c\/tr\u003e\n      \u003c\/thead\u003e\n      \u003ctbody\u003e\n        \u003ctr\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:10px;\"\u003eBSA\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:10px;\"\u003e10 mg\/mL\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:10px;\"\u003e200 mg\/mL\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:10px;\"\u003eOK\u003c\/td\u003e\n\u003c\/tr\u003e\n        \u003ctr\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:10px;\"\u003eSodium acetate\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:10px;\"\u003e20 mM\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:10px;\"\u003e400 mM\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:10px;\"\u003eOK\u003c\/td\u003e\n\u003c\/tr\u003e\n        \u003ctr\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:10px;\"\u003eAmmonium acetate\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:10px;\"\u003e20 mM\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:10px;\"\u003e400 mM\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:10px;\"\u003eOK\u003c\/td\u003e\n\u003c\/tr\u003e\n        \u003ctr\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:10px;\"\u003eSodium chloride\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:10px;\"\u003e20 mM\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:10px;\"\u003e400 mM\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:10px;\"\u003eOK\u003c\/td\u003e\n\u003c\/tr\u003e\n        \u003ctr\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:10px;\"\u003eMagnesium chloride\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:10px;\"\u003e5 mM\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:10px;\"\u003e100 mM\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:10px;\"\u003eOK\u003c\/td\u003e\n\u003c\/tr\u003e\n        \u003ctr\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:10px;\"\u003ePhenol\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:10px;\"\u003e0.1%\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:10px;\"\u003e2%\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:10px;\"\u003eOK\u003c\/td\u003e\n\u003c\/tr\u003e\n        \u003ctr\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:10px;\"\u003eChloroform\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:10px;\"\u003e0.5%\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:10px;\"\u003e10%\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:10px;\"\u003eOK\u003c\/td\u003e\n\u003c\/tr\u003e\n        \u003ctr\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:10px;\"\u003eEthanol\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:10px;\"\u003e0.5%\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:10px;\"\u003e10%\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:10px;\"\u003eOK\u003c\/td\u003e\n\u003c\/tr\u003e\n        \u003ctr\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:10px;\"\u003eSDS\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:10px;\"\u003e0.01%\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:10px;\"\u003e0.2%\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:10px;\"\u003eOK\u003c\/td\u003e\n\u003c\/tr\u003e\n        \u003ctr\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:10px;\"\u003eTriton X-100\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:10px;\"\u003e0.01%\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:10px;\"\u003e0.2%\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:10px;\"\u003eOK\u003c\/td\u003e\n\u003c\/tr\u003e\n        \u003ctr\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:10px;\"\u003essDNA\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:10px;\"\u003e1×\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:10px;\"\u003e1×\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:10px;\"\u003eOK\u003c\/td\u003e\n\u003c\/tr\u003e\n        \u003ctr\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:10px;\"\u003ePEG\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:10px;\"\u003e1%\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:10px;\"\u003e20%\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:10px;\"\u003eOK\u003c\/td\u003e\n\u003c\/tr\u003e\n        \u003ctr\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:10px;\"\u003eRNA\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:10px;\"\u003e1×\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:10px;\"\u003e1×\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:10px;\"\u003eOK\u003c\/td\u003e\n\u003c\/tr\u003e\n        \u003ctr\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:10px;\"\u003edNTPs\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:10px;\"\u003e100 µM\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:10px;\"\u003e2 mM\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:10px;\"\u003eOK\u003c\/td\u003e\n\u003c\/tr\u003e\n      \u003c\/tbody\u003e\n    \u003c\/table\u003e\n  \u003c\/div\u003e\n\u003c\/section\u003e\n\n\u003csection\u003e\n  \u003ch2\u003eApplication\u003c\/h2\u003e\n  \u003cp\u003eThe StarLighter dsDNA HS Assay Kit is intended for fluorescence-based quantification of double-stranded DNA using either a Qubit fluorometer or a fluorescence microplate reader.\u003c\/p\u003e\n\n  \u003ch3\u003eQubit Fluorometer Assay Format\u003c\/h3\u003e\n  \u003col\u003e\n    \u003cli\u003eEquilibrate all kit components to room temperature.\u003c\/li\u003e\n    \u003cli\u003ePrepare the working solution by combining dsDNA HS Fluorescent Reagent QF and dsDNA HS Buffer QB at a 1:199 ratio.\u003c\/li\u003e\n    \u003cli\u003eUse thin-walled, clear 0.5 mL PCR tubes.\u003c\/li\u003e\n    \u003cli\u003eAdd 190 µL of working solution and 10 µL of standard to each standard tube.\u003c\/li\u003e\n    \u003cli\u003eAdd 180–199 µL of working solution and 1–20 µL of sample to each sample tube.\u003c\/li\u003e\n    \u003cli\u003eEnsure that the final volume in every tube is 200 µL.\u003c\/li\u003e\n    \u003cli\u003eVortex for 3–5 seconds without creating bubbles.\u003c\/li\u003e\n    \u003cli\u003eIncubate for 2 minutes at room temperature while protected from light.\u003c\/li\u003e\n    \u003cli\u003eRead the standards and samples using the applicable instrument procedure.\u003c\/li\u003e\n  \u003c\/ol\u003e\n\n  \u003cp\u003eCalibration requires two standards. Standard QS0 is used at 0 ng\/µL, and Standard QS1 is used at 10 ng\/µL. A new calibration is strongly recommended for every new assay run, and calibration curves from other kits must not be used.\u003c\/p\u003e\n\n  \u003ch3\u003eFluorescence Microplate Reader Assay Format\u003c\/h3\u003e\n  \u003col\u003e\n    \u003cli\u003eEquilibrate all kit components to room temperature and mix thoroughly by vortexing.\u003c\/li\u003e\n    \u003cli\u003ePrepare the working solution using a 1:199 ratio of QF fluorescent reagent to QB buffer.\u003c\/li\u003e\n    \u003cli\u003eDispense 190 µL of working solution into standard wells.\u003c\/li\u003e\n    \u003cli\u003eDispense 180–199 µL of working solution into sample wells according to the sample volume.\u003c\/li\u003e\n    \u003cli\u003ePrepare standards from 0 to 10 ng\/µL by serially diluting Standard QS1.\u003c\/li\u003e\n    \u003cli\u003eAdd 10 µL of each standard and 1–20 µL of each sample to the designated wells.\u003c\/li\u003e\n    \u003cli\u003eEnsure that the final volume in each well is exactly 200 µL.\u003c\/li\u003e\n    \u003cli\u003eMix the plate for 10–15 seconds.\u003c\/li\u003e\n    \u003cli\u003eIncubate for 2 minutes at room temperature while protected from light.\u003c\/li\u003e\n    \u003cli\u003eSet the fluorescence microplate reader to 485 nm excitation and 530 nm emission.\u003c\/li\u003e\n    \u003cli\u003eRead the standards and samples, generate a standard curve, and calculate the concentrations of the unknown samples.\u003c\/li\u003e\n  \u003c\/ol\u003e\n\n  \u003cp\u003eBlack-wall, clear-bottom microplates are specified for the 96-well format to minimize cross-talk. Samples exceeding the linear range should be diluted and measured again.\u003c\/p\u003e\n\u003c\/section\u003e\n\n\u003csection\u003e\n  \u003ch2\u003eFAQ\u003c\/h2\u003e\n\n  \u003cdetails\u003e\n    \u003csummary\u003e\u003cstrong\u003eWhat is the StarLighter dsDNA HS Assay Kit used for?\u003c\/strong\u003e\u003c\/summary\u003e\n    \u003cp\u003eThe kit is used for high-sensitivity, fluorescence-based quantification of double-stranded DNA. It provides high selectivity for dsDNA over RNA and supports initial sample concentrations from 5 pg\/µL to 120 ng\/µL, depending on sample volume.\u003c\/p\u003e\n  \u003c\/details\u003e\n\n  \u003cdetails\u003e\n    \u003csummary\u003e\u003cstrong\u003eWhat is the dsDNA quantification range?\u003c\/strong\u003e\u003c\/summary\u003e\n    \u003cp\u003eThe total assay range is 0.1–120 ng. Depending on the sample volume, the supported initial dsDNA concentration range is 5 pg\/µL–120 ng\/µL.\u003c\/p\u003e\n  \u003c\/details\u003e\n\n  \u003cdetails\u003e\n    \u003csummary\u003e\u003cstrong\u003eHow much sample is required per reaction?\u003c\/strong\u003e\u003c\/summary\u003e\n    \u003cp\u003eEach reaction uses 1–20 µL of sample. Working solution is added to produce a final reaction volume of exactly 200 µL.\u003c\/p\u003e\n  \u003c\/details\u003e\n\n  \u003cdetails\u003e\n    \u003csummary\u003e\u003cstrong\u003eCan the kit be used with a Qubit fluorometer?\u003c\/strong\u003e\u003c\/summary\u003e\n    \u003cp\u003eYes. The user guide provides a Qubit fluorometer protocol using thin-walled, clear 0.5 mL PCR tubes, two supplied standards, and a 200 µL final assay volume.\u003c\/p\u003e\n  \u003c\/details\u003e\n\n  \u003cdetails\u003e\n    \u003csummary\u003e\u003cstrong\u003eCan the kit be used with a 96-well fluorescence microplate reader?\u003c\/strong\u003e\u003c\/summary\u003e\n    \u003cp\u003eYes. The protocol supports black-wall, clear-bottom 96-well microplates. The specified reader settings are 485 nm excitation and 530 nm emission.\u003c\/p\u003e\n  \u003c\/details\u003e\n\n  \u003cdetails\u003e\n    \u003csummary\u003e\u003cstrong\u003eHow long is the incubation time?\u003c\/strong\u003e\u003c\/summary\u003e\n    \u003cp\u003eThe assay requires a 2-minute incubation at room temperature while protected from light. The fluorescent signal remains stable for up to 3 hours after incubation when protected from light.\u003c\/p\u003e\n  \u003c\/details\u003e\n\n  \u003cdetails\u003e\n    \u003csummary\u003e\u003cstrong\u003eWhich kit sizes and catalog numbers are available?\u003c\/strong\u003e\u003c\/summary\u003e\n    \u003cp\u003eFS-T1001-S contains reagents for 20 reactions. FS-T1002 contains reagents for 500 reactions.\u003c\/p\u003e\n  \u003c\/details\u003e\n\n  \u003cdetails\u003e\n    \u003csummary\u003e\u003cstrong\u003eHow should the StarLighter dsDNA HS Assay Kit be shipped and stored?\u003c\/strong\u003e\u003c\/summary\u003e\n    \u003cp\u003eThe kit should be shipped on ice and stored at 2–8°C upon arrival. Fluorescent reagents must be protected from light to prevent signal decay.\u003c\/p\u003e\n  \u003c\/details\u003e\n\u003c\/section\u003e","brand":"Qihengxing","offers":[{"title":"20 Reactions","offer_id":53189979177141,"sku":"FS-T1001-S","price":0.0,"currency_code":"USD","in_stock":false},{"title":"500 reactions","offer_id":53189979209909,"sku":"FS-T1002","price":0.0,"currency_code":"USD","in_stock":false}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/exec-ad012ae6-7232-4929-b4b0-121a0bdbda05.png?v=1788164333","url":"https:\/\/biofargo.com\/products\/dsdna-hs-assay-kit-starlighter","provider":"Biofargo","version":"1.0","type":"link"}