Description

Laboratories measuring double-stranded DNA by UV absorbance at 260 nm may encounter interference from RNA, proteins, salts, and detergents. The Biofargo dsDNA BR Assay Kit uses a fluorescence-based method that is highly selective for dsDNA over RNA and is minimally affected by the common contaminants evaluated in the user guide.

Depending on sample volume, the assay measures initial dsDNA concentrations from 0.2 to 1,000 ng/µL, with a total assay range of 2–1,000 ng. Each assay uses 1–20 µL of sample in a final volume of 200 µL. The protocol supports a Qubit fluorometer or a fluorescence microplate reader.

The assay requires a 2-minute incubation at room temperature protected from light. The signal remains stable for up to 3 hours after incubation when protected from light. For research use only; not for diagnostic or therapeutic procedures.

Specifications

Parameter	Specification
Product name	Biofargo dsDNA BR Assay Kit
Target	Double-stranded DNA (dsDNA)
Detection method	Fluorescence
Initial dsDNA concentration	0.2–1,000 ng/µL, depending on sample volume
Total assay range	2–1,000 ng
Sample volume	1–20 µL
Final assay volume	200 µL per tube or well
Assay temperature	20–25 °C
Incubation	2 minutes, protected from light
Compatible instruments	Qubit fluorometer or fluorescence microplate reader
Microplate reader settings	485 nm excitation; 530 nm emission
Available formats	FS-T1004-S: 100 reactions; FS-T1004: 1,000 reactions
Intended use	Research use only

Features

Broad-range dsDNA measurement with a total assay range of 2–1,000 ng.
Highly selective for double-stranded DNA over RNA.
Accepts 1–20 µL of sample in a final assay volume of 200 µL.
Protocols for a Qubit fluorometer and a fluorescence microplate reader.
A 2-minute incubation at room temperature, protected from light.
Fluorescent signal remains stable for up to 3 hours after incubation when protected from light.

Applications

Qubit Fluorometer: Quantify dsDNA in clear, thin-wall 0.5 mL PCR tubes using the two supplied standards and the dsDNA Broad Range assay setting.
Fluorescence Microplate Reader: Quantify dsDNA in a black-wall, clear-bottom 96-well microplate using a standard curve, 485 nm excitation, and 530 nm emission.

Kit Components

Component Name	FS-T1004-S (100 reactions)	FS-T1004 (1,000 reactions)
dsDNA BR Buffer QB	10 mL	200 mL
dsDNA BR Standard QS0	100 µL, 0 ng/µL	1 mL, 0 ng/µL
dsDNA BR Standard QS1	100 µL, 100 ng/µL	1 mL, 100 ng/µL
dsDNA BR Fluorescent Reagent QF	100 µL	1 mL

Storage & Shipping

Ship on ice.
Store at 2–8 °C upon arrival.
Protect the fluorescent dye and working solution from light.
Prepare the working solution fresh for each assay.

Frequently Asked Questions (FAQs)

What is the detection range of the Biofargo dsDNA BR Assay Kit?

The total assay range is 2–1,000 ng. Depending on sample volume, the initial dsDNA concentration range is 0.2–1,000 ng/µL.

Can the kit be used with a Qubit fluorometer?

Yes. The user guide includes a Qubit workflow using two standards. On a Qubit 4 Fluorometer, select dsDNA and then dsDNA Broad Range.

Can the kit be used with a fluorescence microplate reader?

Yes. The user guide includes a 96-well workflow with 485 nm excitation and 530 nm emission.

How much sample is needed?

Add 1–20 µL of sample and adjust the working solution so that the final volume is 200 µL per tube or well.

How should the kit be stored?

The kit is shipped on ice and should be stored at 2–8 °C upon arrival. Protect the fluorescent dye and freshly prepared working solution from light.

Documents

Product User Manual