{"product_id":"dsdna-br-assay-kit-starlighter","title":"dsDNA BR Assay Kit——StarLighter","description":"\u003csection\u003e\n  \u003ch2\u003eDescription\u003c\/h2\u003e\n\n  \u003cp\u003eLaboratories quantifying double-stranded DNA may encounter interference when using traditional UV absorbance at 260 nm. RNA, proteins, salts, detergents, and other substances can affect absorbance-based measurements, particularly when samples must be measured across a broad concentration range.\u003c\/p\u003e\n\n  \u003cp\u003eThe StarLighter dsDNA BR Assay Kit provides a fluorescence-based solution for broad-range dsDNA quantification. The assay is highly selective for double-stranded DNA over RNA and supports initial dsDNA concentrations from 0.2 ng\/µL to 1,000 ng\/µL, depending on the sample volume. Its total assay range is 2–1,000 ng.\u003c\/p\u003e\n\n  \u003cp\u003eThis dsDNA broad range assay kit requires only 1–20 µL of sample in a final assay volume of 200 µL. It can be used with either a Qubit fluorometer or a fluorescence microplate reader, allowing laboratories to perform individual tube measurements or 96-well fluorescence-based dsDNA quantitation.\u003c\/p\u003e\n\n  \u003cp\u003eThe assay is performed at room temperature after a 2-minute incubation protected from light. When protected from light, the fluorescent signal remains stable for up to 3 hours after incubation.\u003c\/p\u003e\n\n  \u003cp\u003e\u003cstrong\u003eFor Research Use Only. Not for use in diagnostic or therapeutic procedures.\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/section\u003e\n\n\u003csection\u003e\n  \u003ch2\u003eSpecification\u003c\/h2\u003e\n\n  \u003cdiv style=\"width:100%; overflow-x:auto; -webkit-overflow-scrolling:touch;\"\u003e\n    \u003ctable style=\"width:100%; min-width:680px; border-collapse:collapse;\"\u003e\n      \u003ctbody\u003e\n        \u003ctr\u003e\n          \u003cth style=\"text-align:left; padding:10px; border:1px solid #ddd;\"\u003eProduct Name\u003c\/th\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #ddd;\"\u003eStarLighter dsDNA BR Assay Kit\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003cth style=\"text-align:left; padding:10px; border:1px solid #ddd;\"\u003eAssay Type\u003c\/th\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #ddd;\"\u003eBroad Range dsDNA Assay\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003cth style=\"text-align:left; padding:10px; border:1px solid #ddd;\"\u003eTarget\u003c\/th\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #ddd;\"\u003eDouble-stranded DNA (dsDNA)\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003cth style=\"text-align:left; padding:10px; border:1px solid #ddd;\"\u003eDetection Method\u003c\/th\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #ddd;\"\u003eFluorescence\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003cth style=\"text-align:left; padding:10px; border:1px solid #ddd;\"\u003eInitial Sample Concentration\u003c\/th\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #ddd;\"\u003e0.2–1,000 ng\/µL, depending on sample volume\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003cth style=\"text-align:left; padding:10px; border:1px solid #ddd;\"\u003eTotal Assay Range\u003c\/th\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #ddd;\"\u003e2–1,000 ng\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003cth style=\"text-align:left; padding:10px; border:1px solid #ddd;\"\u003eSample Volume\u003c\/th\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #ddd;\"\u003e1–20 µL\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003cth style=\"text-align:left; padding:10px; border:1px solid #ddd;\"\u003eFinal Assay Volume\u003c\/th\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #ddd;\"\u003e200 µL per tube or well\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003cth style=\"text-align:left; padding:10px; border:1px solid #ddd;\"\u003eAssay Temperature\u003c\/th\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #ddd;\"\u003eRoom temperature, 20–25°C\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003cth style=\"text-align:left; padding:10px; border:1px solid #ddd;\"\u003eIncubation\u003c\/th\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #ddd;\"\u003e2 minutes at room temperature, protected from light\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003cth style=\"text-align:left; padding:10px; border:1px solid #ddd;\"\u003eSignal Stability\u003c\/th\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #ddd;\"\u003eUp to 3 hours after incubation when protected from light\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003cth style=\"text-align:left; padding:10px; border:1px solid #ddd;\"\u003eCompatible Instruments\u003c\/th\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #ddd;\"\u003eQubit fluorometer or fluorescence microplate reader\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003cth style=\"text-align:left; padding:10px; border:1px solid #ddd;\"\u003eMicroplate Reader Settings\u003c\/th\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #ddd;\"\u003eExcitation: 485 nm; Emission: 530 nm\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003cth style=\"text-align:left; padding:10px; border:1px solid #ddd;\"\u003eWorking Solution\u003c\/th\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #ddd;\"\u003eQF:QB = 1:199, equivalent to a 1:200 final dilution\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003cth style=\"text-align:left; padding:10px; border:1px solid #ddd;\"\u003eShipping Condition\u003c\/th\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #ddd;\"\u003eShip on ice\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003cth style=\"text-align:left; padding:10px; border:1px solid #ddd;\"\u003eStorage\u003c\/th\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #ddd;\"\u003e2–8°C upon arrival\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003cth style=\"text-align:left; padding:10px; border:1px solid #ddd;\"\u003eIntended Use\u003c\/th\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #ddd;\"\u003eResearch Use Only\u003c\/td\u003e\n        \u003c\/tr\u003e\n      \u003c\/tbody\u003e\n    \u003c\/table\u003e\n  \u003c\/div\u003e\n\n  \u003ch3\u003eAvailable Sizes and Kit Components\u003c\/h3\u003e\n\n  \u003cdiv style=\"width:100%; overflow-x:auto; -webkit-overflow-scrolling:touch;\"\u003e\n    \u003ctable style=\"width:100%; min-width:760px; border-collapse:collapse;\"\u003e\n      \u003cthead\u003e\n        \u003ctr\u003e\n          \u003cth style=\"text-align:left; padding:10px; border:1px solid #ddd;\"\u003eComponent\u003c\/th\u003e\n          \u003cth style=\"text-align:left; padding:10px; border:1px solid #ddd;\"\u003eFS-T1004-S — 100 Reactions\u003c\/th\u003e\n          \u003cth style=\"text-align:left; padding:10px; border:1px solid #ddd;\"\u003eFS-T1004 — 1,000 Reactions\u003c\/th\u003e\n        \u003c\/tr\u003e\n      \u003c\/thead\u003e\n      \u003ctbody\u003e\n        \u003ctr\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #ddd;\"\u003edsDNA BR Buffer QB\u003c\/td\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #ddd;\"\u003e10 mL\u003c\/td\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #ddd;\"\u003e200 mL\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #ddd;\"\u003edsDNA BR Standard QS0\u003c\/td\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #ddd;\"\u003e100 µL, 0 ng\/µL\u003c\/td\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #ddd;\"\u003e1 mL, 0 ng\/µL\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #ddd;\"\u003edsDNA BR Standard QS1\u003c\/td\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #ddd;\"\u003e100 µL, 100 ng\/µL\u003c\/td\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #ddd;\"\u003e1 mL, 100 ng\/µL\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #ddd;\"\u003edsDNA BR Fluorescent Reagent QF\u003c\/td\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #ddd;\"\u003e100 µL\u003c\/td\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #ddd;\"\u003e1 mL\u003c\/td\u003e\n        \u003c\/tr\u003e\n      \u003c\/tbody\u003e\n    \u003c\/table\u003e\n  \u003c\/div\u003e\n\u003c\/section\u003e\n\n\u003csection\u003e\n  \u003ch2\u003eFeatures\u003c\/h2\u003e\n\n  \u003cul\u003e\n    \u003cli\u003e\n\u003cstrong\u003eBroad-range dsDNA quantification:\u003c\/strong\u003e The fluorescent dsDNA quantitation kit covers a total assay range of 2–1,000 ng.\u003c\/li\u003e\n    \u003cli\u003e\n\u003cstrong\u003eSelective dsDNA measurement:\u003c\/strong\u003e The assay is highly selective for double-stranded DNA over RNA.\u003c\/li\u003e\n    \u003cli\u003e\n\u003cstrong\u003eFlexible sample input:\u003c\/strong\u003e The assay accepts 1–20 µL of sample in a final volume of 200 µL.\u003c\/li\u003e\n    \u003cli\u003e\n\u003cstrong\u003eTwo instrument formats:\u003c\/strong\u003e The kit can be used with a Qubit fluorometer or a fluorescence microplate reader.\u003c\/li\u003e\n    \u003cli\u003e\n\u003cstrong\u003eShort incubation:\u003c\/strong\u003e Fluorescent signal development requires 2 minutes at room temperature while protected from light.\u003c\/li\u003e\n    \u003cli\u003e\n\u003cstrong\u003eStable reading window:\u003c\/strong\u003e The signal remains stable for up to 3 hours after incubation when protected from light.\u003c\/li\u003e\n    \u003cli\u003e\n\u003cstrong\u003eContaminant tolerance:\u003c\/strong\u003e The assay was evaluated with common laboratory contaminants, including salts, solvents, detergents, protein, agarose, PEG, and dNTPs.\u003c\/li\u003e\n    \u003cli\u003e\n\u003cstrong\u003eTwo kit sizes:\u003c\/strong\u003e The dsDNA BR assay kit is available in 100-reaction and 1,000-reaction formats.\u003c\/li\u003e\n  \u003c\/ul\u003e\n\n  \u003ch3\u003eContaminant Tolerance\u003c\/h3\u003e\n\n  \u003cp\u003eDNA standards were tested in the presence or absence of the contaminants shown below. An “OK” result usually represents less than 10% perturbation at the indicated final assay concentration.\u003c\/p\u003e\n\n  \u003cdiv style=\"width:100%; overflow-x:auto; -webkit-overflow-scrolling:touch;\"\u003e\n    \u003ctable style=\"width:100%; min-width:760px; border-collapse:collapse;\"\u003e\n      \u003cthead\u003e\n        \u003ctr\u003e\n          \u003cth style=\"text-align:left; padding:10px; border:1px solid #ddd;\"\u003eContaminant\u003c\/th\u003e\n          \u003cth style=\"text-align:left; padding:10px; border:1px solid #ddd;\"\u003eFinal Concentration in Assay\u003c\/th\u003e\n          \u003cth style=\"text-align:left; padding:10px; border:1px solid #ddd;\"\u003eApproximate Concentration in 10 µL Sample\u003c\/th\u003e\n          \u003cth style=\"text-align:left; padding:10px; border:1px solid #ddd;\"\u003eResult\u003c\/th\u003e\n        \u003c\/tr\u003e\n      \u003c\/thead\u003e\n      \u003ctbody\u003e\n        \u003ctr\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #ddd;\"\u003eBSA\u003c\/td\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #ddd;\"\u003e10 mg\/mL\u003c\/td\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #ddd;\"\u003e200 mg\/mL\u003c\/td\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #ddd;\"\u003eOK\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #ddd;\"\u003eSodium Acetate\u003c\/td\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #ddd;\"\u003e20 mM\u003c\/td\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #ddd;\"\u003e400 mM\u003c\/td\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #ddd;\"\u003eOK\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #ddd;\"\u003eAmmonium Acetate\u003c\/td\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #ddd;\"\u003e20 mM\u003c\/td\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #ddd;\"\u003e400 mM\u003c\/td\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #ddd;\"\u003eOK\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #ddd;\"\u003eSodium Chloride\u003c\/td\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #ddd;\"\u003e20 mM\u003c\/td\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #ddd;\"\u003e400 mM\u003c\/td\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #ddd;\"\u003eOK\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #ddd;\"\u003eMagnesium Chloride\u003c\/td\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #ddd;\"\u003e5 mM\u003c\/td\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #ddd;\"\u003e100 mM\u003c\/td\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #ddd;\"\u003eOK\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #ddd;\"\u003ePhenol\u003c\/td\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #ddd;\"\u003e0.1%\u003c\/td\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #ddd;\"\u003e2%\u003c\/td\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #ddd;\"\u003eOK\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #ddd;\"\u003eChloroform\u003c\/td\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #ddd;\"\u003e0.5%\u003c\/td\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #ddd;\"\u003e10%\u003c\/td\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #ddd;\"\u003eOK\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #ddd;\"\u003eEthanol\u003c\/td\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #ddd;\"\u003e0.5%\u003c\/td\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #ddd;\"\u003e10%\u003c\/td\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #ddd;\"\u003eOK\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #ddd;\"\u003eSodium Dodecyl Sulfate (SDS)\u003c\/td\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #ddd;\"\u003e0.01%\u003c\/td\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #ddd;\"\u003e0.2%\u003c\/td\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #ddd;\"\u003eOK\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #ddd;\"\u003eTriton X-100\u003c\/td\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #ddd;\"\u003e0.01%\u003c\/td\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #ddd;\"\u003e0.2%\u003c\/td\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #ddd;\"\u003eOK\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #ddd;\"\u003eAgarose\u003c\/td\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #ddd;\"\u003e0.1%\u003c\/td\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #ddd;\"\u003e2%\u003c\/td\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #ddd;\"\u003eOK\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #ddd;\"\u003ePolyethylene Glycol (PEG)\u003c\/td\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #ddd;\"\u003e1%\u003c\/td\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #ddd;\"\u003e20%\u003c\/td\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #ddd;\"\u003eOK\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #ddd;\"\u003edNTPs\u003c\/td\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #ddd;\"\u003e100 µM\u003c\/td\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #ddd;\"\u003e2 mM\u003c\/td\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #ddd;\"\u003eOK\u003c\/td\u003e\n        \u003c\/tr\u003e\n      \u003c\/tbody\u003e\n    \u003c\/table\u003e\n  \u003c\/div\u003e\n\u003c\/section\u003e\n\n\u003csection\u003e\n  \u003ch2\u003eApplication\u003c\/h2\u003e\n\n  \u003ch3\u003eBroad-Range dsDNA Quantification with a Qubit Fluorometer\u003c\/h3\u003e\n\n  \u003col\u003e\n    \u003cli\u003eEquilibrate all kit components to room temperature before use.\u003c\/li\u003e\n    \u003cli\u003ePrepare two standard tubes and the required number of sample tubes using thin-wall, clear 0.5 mL PCR tubes.\u003c\/li\u003e\n    \u003cli\u003ePrepare fresh Working Solution by combining dsDNA BR Fluorescent Reagent QF and dsDNA BR Buffer QB at a QF:QB ratio of 1:199.\u003c\/li\u003e\n    \u003cli\u003eDo not prepare or mix the Working Solution in a glass container.\u003c\/li\u003e\n    \u003cli\u003eAdd 190 µL of Working Solution to each standard tube.\u003c\/li\u003e\n    \u003cli\u003eAdd 180–199 µL of Working Solution to each sample tube, depending on the sample volume.\u003c\/li\u003e\n    \u003cli\u003eAdd 10 µL of QS0 to the first standard tube and 10 µL of QS1 to the second standard tube.\u003c\/li\u003e\n    \u003cli\u003eAdd 1–20 µL of sample to the appropriate sample tube. The final volume must be 200 µL.\u003c\/li\u003e\n    \u003cli\u003eVortex vigorously for 3–5 seconds without creating bubbles.\u003c\/li\u003e\n    \u003cli\u003eIncubate at room temperature for 2 minutes while protected from light.\u003c\/li\u003e\n    \u003cli\u003eOn a Qubit 4 Fluorometer, select \u003cstrong\u003edsDNA\u003c\/strong\u003e and then \u003cstrong\u003edsDNA Broad Range\u003c\/strong\u003e.\u003c\/li\u003e\n    \u003cli\u003eRead the two standards in the correct order before reading the samples.\u003c\/li\u003e\n  \u003c\/ol\u003e\n\n  \u003cp\u003eA new calibration is strongly recommended for every assay run. Calibration curves generated using other kits must not be used.\u003c\/p\u003e\n\n  \u003ch3\u003eBroad-Range dsDNA Quantification with a Fluorescence Microplate Reader\u003c\/h3\u003e\n\n  \u003col\u003e\n    \u003cli\u003eEquilibrate all kit components to room temperature and mix thoroughly by vortexing.\u003c\/li\u003e\n    \u003cli\u003ePrepare fresh Working Solution by combining QF and QB at a QF:QB ratio of 1:199.\u003c\/li\u003e\n    \u003cli\u003eUse a black-wall, clear-bottom 96-well microplate to minimize cross-talk.\u003c\/li\u003e\n    \u003cli\u003eAdd 190 µL of Working Solution to each standard well.\u003c\/li\u003e\n    \u003cli\u003eAdd 180–199 µL of Working Solution to each sample well, depending on the sample volume.\u003c\/li\u003e\n    \u003cli\u003eSerially dilute Standard QS1 to prepare standards ranging from 0 to 100 ng\/µL.\u003c\/li\u003e\n    \u003cli\u003eAdd 10 µL of each standard to the designated standard wells.\u003c\/li\u003e\n    \u003cli\u003eAdd 1–20 µL of each sample to the corresponding wells. The final volume must be exactly 200 µL per well.\u003c\/li\u003e\n    \u003cli\u003eVortex the plate briefly for 10–15 seconds.\u003c\/li\u003e\n    \u003cli\u003eIncubate the plate for 2 minutes at room temperature while protected from light.\u003c\/li\u003e\n    \u003cli\u003eSet the microplate reader to 485 nm excitation and 530 nm emission.\u003c\/li\u003e\n    \u003cli\u003eRead the standards and samples, generate a standard curve, and calculate the concentrations of the unknown samples.\u003c\/li\u003e\n  \u003c\/ol\u003e\n\n  \u003cp\u003eIf a sample exceeds the assay range, dilute the sample appropriately and repeat the assay.\u003c\/p\u003e\n\u003c\/section\u003e\n\n\u003csection\u003e\n  \u003ch2\u003eFAQ\u003c\/h2\u003e\n\n  \u003cdetails\u003e\n    \u003csummary\u003e\u003cstrong\u003eWhat is the detection range of the StarLighter dsDNA BR Assay Kit?\u003c\/strong\u003e\u003c\/summary\u003e\n    \u003cp\u003eThe total assay range is 2–1,000 ng. Depending on the sample volume, the supported initial dsDNA concentration range is 0.2–1,000 ng\/µL.\u003c\/p\u003e\n  \u003c\/details\u003e\n\n  \u003cdetails\u003e\n    \u003csummary\u003e\u003cstrong\u003eIs this dsDNA broad range assay kit compatible with a Qubit fluorometer?\u003c\/strong\u003e\u003c\/summary\u003e\n    \u003cp\u003eYes. The kit includes a protocol for Qubit fluorometers. On a Qubit 4 Fluorometer, select dsDNA and then dsDNA Broad Range as the assay type.\u003c\/p\u003e\n  \u003c\/details\u003e\n\n  \u003cdetails\u003e\n    \u003csummary\u003e\u003cstrong\u003eCan the kit be used with a fluorescence microplate reader?\u003c\/strong\u003e\u003c\/summary\u003e\n    \u003cp\u003eYes. The kit includes a 96-well microplate workflow. Use a black-wall, clear-bottom microplate and set the reader to 485 nm excitation and 530 nm emission.\u003c\/p\u003e\n  \u003c\/details\u003e\n\n  \u003cdetails\u003e\n    \u003csummary\u003e\u003cstrong\u003eHow much sample is required for each dsDNA assay?\u003c\/strong\u003e\u003c\/summary\u003e\n    \u003cp\u003eEach reaction requires 1–20 µL of sample. The final assay volume must be 200 µL per tube or well. When adding only 1–2 µL of sample, a 2 µL pipette is recommended.\u003c\/p\u003e\n  \u003c\/details\u003e\n\n  \u003cdetails\u003e\n    \u003csummary\u003e\u003cstrong\u003eHow should the Working Solution be prepared?\u003c\/strong\u003e\u003c\/summary\u003e\n    \u003cp\u003ePrepare the Working Solution by combining dsDNA BR Fluorescent Reagent QF and dsDNA BR Buffer QB at a QF:QB ratio of 1:199, equivalent to a 1:200 final dilution. Prepare it fresh for each assay, protect it from light, and do not mix it in a glass container.\u003c\/p\u003e\n  \u003c\/details\u003e\n\n  \u003cdetails\u003e\n    \u003csummary\u003e\u003cstrong\u003eWhat kit sizes and catalog numbers are available?\u003c\/strong\u003e\u003c\/summary\u003e\n    \u003cp\u003eFS-T1004-S contains reagents for 100 reactions. FS-T1004 contains reagents for 1,000 reactions.\u003c\/p\u003e\n  \u003c\/details\u003e\n\n  \u003cdetails\u003e\n    \u003csummary\u003e\u003cstrong\u003eHow should the StarLighter dsDNA BR Assay Kit be stored?\u003c\/strong\u003e\u003c\/summary\u003e\n    \u003cp\u003eThe kit should be shipped on ice and stored at 2–8°C upon arrival. The fluorescent dye and prepared Working Solution must be protected from light.\u003c\/p\u003e\n  \u003c\/details\u003e\n\n  \u003cdetails\u003e\n    \u003csummary\u003e\u003cstrong\u003eHow long is the fluorescence signal stable?\u003c\/strong\u003e\u003c\/summary\u003e\n    \u003cp\u003eAfter the 2-minute incubation, the fluorescent signal remains stable for up to 3 hours when protected from light.\u003c\/p\u003e\n  \u003c\/details\u003e\n\u003c\/section\u003e","brand":"Qihengxing","offers":[{"title":"100 Reactions","offer_id":53189995823285,"sku":"FS-T1004S","price":0.0,"currency_code":"USD","in_stock":false},{"title":"1000 Reactions","offer_id":53189995856053,"sku":"FS-T1004","price":0.0,"currency_code":"USD","in_stock":false}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/exec-1799be62-88be-4aa5-9c26-780868e217db.png?v=1788166916","url":"https:\/\/biofargo.com\/products\/dsdna-br-assay-kit-starlighter","provider":"Biofargo","version":"1.0","type":"link"}