Synonym(s): 3-(N,N-Bis[2-hydroxyethyl]amino)-2-hydroxypropanesulfonic acid, N,N-Bis(2-hydroxyethyl)-3-amino-2-hydroxypropanesulfonic acid

Description

DIPSO (3-[N,N-Bis(2-hydroxyethyl)amino]-2-hydroxy-1-propanesulfonic acid) is a high-purity zwitterionic buffering agent widely used in biochemical and molecular biology workflows. With excellent solubility, low UV absorbance, and minimal metal ion contamination, DIPSO provides stable buffering capacity in the near-neutral pH range. Its biochemical-grade specifications make it suitable for protein purification, enzyme assays, cell-free systems, and other sensitive laboratory applications requiring high-performance buffering.

Specifications

Item	Value
Chemical Name	3-[N,N-Bis(2-Hydroxyethyl)Amino]-2-Hydroxy-1-Propanesulfonic Acid
Appearance	White Powder
CAS No.	68399-80-4
Molecular Formula	C7H17NO6S
Molecular Weight	243.28
Grade	Biochemical Grade
Solubility	Colorless, Clear
Water-Insoluble Matter	≤0.1%
pH	4.0–5.5
Ammonia	≤0.05%
Ignition Residue	≤0.1%
Chlorides	≤0.05%
Sulfates	≤0.05%
Aluminum	≤0.0005%
Lead	≤0.0001%
Phosphorus	≤0.0005%
Copper	≤0.0005%
Iron	≤0.0005%
Zinc	≤0.0005%
Magnesium	≤0.0005%
Calcium	≤0.001%
Sodium	≤0.01%
Potassium	≤0.005%
UV Absorbance at 260nm	≤0.025
UV Absorbance at 280nm	≤0.025

Features

High-purity biochemical grade with tightly controlled impurities and extremely low metal ion content.
Excellent solubility in aqueous solutions, forming a colorless, clear buffer.
Low UV absorbance at 260/280 nm, ideal for nucleic acid or protein studies.
Stable buffering performance in the pH range required for many biochemical reactions.
Consistent quality ensuring reproducible results in analytical, molecular, and enzymatic workflows.

Applications

Buffer preparation for protein purification, chromatography, and electrophoresis.
Enzyme assays requiring stable pH conditions.
Cell-free biochemical systems, molecular biology reactions, and diagnostic platforms.
Suitable for spectrophotometric applications due to low UV interference.
General-purpose buffer use in research laboratories.

Safety Disclaimer

This product is intended for laboratory research use only. Not for drug, human, or veterinary use. Review the Safety Data Sheet (SDS) provided by BioFargo for detailed handling, storage, hazard, and disposal information. Use appropriate personal protective equipment (PPE) including gloves, lab coat, and eye protection when handling. Dispose of waste and residual buffer in accordance with local, state, and federal regulations. Ensure proper ventilation if preparing large volumes or heating solutions.

Documents

SDS

DIPSO 98% Biochemical Grade