{"product_id":"diabetic-mouse-pulmonary-vein-endothelial-cells","title":"Diabetic Mouse Pulmonary Vein Endothelial Cells","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eDiabetic\u003cstrong\u003e \u003c\/strong\u003eMouse Primary Pulmonary Vein Endothelial Cells from Cell Biologics are isolated from pulmonary vein of pathogen-free Diabetic (db\/db) mice and grown in gelatin pre-coated tissue culture flask with Cell Biologics’ Complete Growth Medium. Cells at passage 3 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x10\u003csup\u003e6\u003c\/sup\u003e cells per ml and is delivered frozen. Diabetic\u003cstrong\u003e \u003c\/strong\u003eMouse Primary Pulmonary Vein Endothelial Cells are characterized by immunofluorescence staining with antibodies of VE-cadherin (DIABETIC44, VE-cadherin Antibody, C-19, sc6458, Santa Cruz), AF1002 (R\u0026amp;D System) or CD31\/PECAM-1 (Catalog No. 553370, BD). These cells are negative for bacteria, yeast, fungi, and mycoplasma and can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells are not recommended.\u003c\/p\u003e\n\u003ch3\u003eSuggested Medium\u003c\/h3\u003e\n\u003cp\u003eM1168 Complete Endothelial Cell Medium w\/ Kit – 500 ml\u003c\/p\u003e\n\u003ch2\u003eStorage\u003c\/h2\u003e\n\u003cp\u003eCryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/files.biofargo.com\/cell-biologics\/pdfs\/MD-6060_MD-6060.pdf\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eMD-6060\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/files.biofargo.com\/cell-biologics\/pdfs\/MD-6060_Cell-Culture-Protocol-CB.pdf\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eCell-Culture-Protocol-CB\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003ch2\u003eDisclaimer\u003c\/h2\u003e\n\u003cp\u003eInvestigators should handle the cells with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.\u003c\/p\u003e\n","brand":"Cell Biologics","offers":[{"title":"Frozen Vial","offer_id":52481675788469,"sku":"MD-6060","price":620.0,"currency_code":"USD","in_stock":true},{"title":"T25 Flask","offer_id":52481675821237,"sku":"MD-6060-1","price":670.0,"currency_code":"USD","in_stock":true},{"title":"T75 Flask","offer_id":52481675854005,"sku":"MD-6060-2","price":890.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/IMG_0268AA-500x500.jpg?v=1778250477","url":"https:\/\/biofargo.com\/products\/diabetic-mouse-pulmonary-vein-endothelial-cells","provider":"Biofargo","version":"1.0","type":"link"}