{"product_id":"diabetic-mouse-ovarian-microvascular-endothelial-cells","title":"Diabetic Mouse Ovarian Microvascular Endothelial Cells","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eDiabetic Mouse Ovarian Microvascular Endothelial Cells from \u003ci\u003eCell Biologics\u003c\/i\u003e are isolated from the ovary of Diabetic (db\/db) mice. Diabetic Mouse Ovarian Microvascular Endothelial Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 2 min and incubated in \u003ci\u003eCell Biologics’\u003c\/i\u003e Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells at passage 3 are detached from flasks and immediately cryopreserved in vials. Each vial contains at least 0.5×10\u003csup\u003e6\u003c\/sup\u003e cells per ml. The method we use to isolate primary endothelial cells was developed based on a combination of established and our proprietary methods. These cells are pre-coated with PECAM-1 antibody, followed by the application of magnetic beads pre-coated with secondary antibody.\u003c\/p\u003e\n\u003cp\u003e\u003cb\u003eProduct Testing\u003c\/b\u003e\u003cb\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003cp\u003eDiabetic Mouse Ovarian Microvascular Endothelial Cells are tested for expression of markers using antibody, VE-cadherin (CD144, VE-cadherin Antibody, C-19, sc6458, Santa Cruz); AF1002 (R\u0026amp;D System) or CD31\/PECAM-1 (Purified Rat Anti-Mouse CD31, Catalog No. 553370, BD) by immunofluorescence staining or FACS. Cells are negative for bacteria, yeast, fungi and mycoplasma. Cells can be expanded for 3-7 passages at a split ratio of 1:2 under the cell culture conditions specified by \u003ci\u003eCell Biologics.\u003c\/i\u003e Repeated freezing and thawing of cells is not recommended.\u003cb\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003ch3\u003eSuggested Medium\u003c\/h3\u003e\n\u003cp\u003eEndothelial Cell Medium \/w Kit – 500 ml\u003c\/p\u003e\n\u003cp\u003eCatalog No.  \u003cb\u003eM1168\u003c\/b\u003e\u003c\/p\u003e\n\u003ch2\u003eLaboratory Applications\u003c\/h2\u003e\n\u003cp\u003eDiabetic Mouse Ovarian Microvascular Endothelial Cells can be used in assays of cell to cell adhesion, migration, vascular tube formation. Standard biochemical procedures performed with endothelial cell cultures include RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining, immunofluorescent flow cytometry, or generating cell derivatives for desired research applications.\u003c\/p\u003e\n\u003ch2\u003eStorage\u003c\/h2\u003e\n\u003cp\u003e\u003ci\u003eCell Biologics\u003c\/i\u003e ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use. Live cell shipment is also available on request. Primary cells should never be kept at -20 °C.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/files.biofargo.com\/cell-biologics\/pdfs\/MD-6090_MD-6090.pdf\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eMD-6090\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/files.biofargo.com\/cell-biologics\/pdfs\/MD-6090_Cell-Culture-Protocol-CB.pdf\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eCell-Culture-Protocol-CB\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003ch2\u003eDisclaimer\u003c\/h2\u003e\n\u003cp\u003eAppropriate safety procedures should always be used with this material. Investigators should handle the cells that they receive from \u003ci\u003eCell Biologics\u003c\/i\u003e with caution and treat all primary cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents. The entire text of discussing Biosafety in Microbiological and Biomedical Laboratories, 5th ed. is available online at http:\/\/www.cdc.gov\/biosafety\/publications\/bmbl5\/index.htm.\u003c\/p\u003e\n","brand":"Cell Biologics","offers":[{"title":"Frozen Vial","offer_id":52481663991989,"sku":"MD-6090","price":620.0,"currency_code":"USD","in_stock":true},{"title":"T25 Flask","offer_id":52481664024757,"sku":"MD-6090-1","price":670.0,"currency_code":"USD","in_stock":true},{"title":"T75 Flask","offer_id":52481664057525,"sku":"MD-6090-2","price":890.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/IMG_0274A-500x500_ce903783-4db8-4763-a5d0-e4ae152aed4a.jpg?v=1778252277","url":"https:\/\/biofargo.com\/products\/diabetic-mouse-ovarian-microvascular-endothelial-cells","provider":"Biofargo","version":"1.0","type":"link"}