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DH5α Chemically Competent Cell from YEASEN is a competent cell obtained by a special process of DH5α strain. It can be used for the chemical transformation of DNA with a conversion efficiency of up to 108 cfu/μg plasmid DNA. It is widely used for efficient DNA cloning and plasmid amplification and can ensure the stable inheritance of high-copy plasmids. At the same time, it can be used to construct clones and blue-white spot screening. Deletion of recA1 and endA1 genes in DH5α cells ensured the stability of cloned DNA.
DH5α competent cell genotypes: F- φ80 lac ZΔM15 Δ(lacZYA-arg F) U169 endA1 recA1 hsdR17(rk-,mk+) supE44λ- thi -1 gyrA96 relA1phoA
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