Description

Cynomolgus Monkey Primary Skeletal Muscle Cells from Cell Biologics are isolated from Cynomolgus Monkey skeletal muscle tissue. Cynomolgus Monkey Primary Skeletal Muscle Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based coating solution for 2 min and incubated in Cell Biologics Culture Complete Growth Medium generally for 3-7 days. Prior to shipping, cells at passage 2-3 are detached from the culture flasks and immediately cryo-preserved in vials. Each vial contains at least 0.5x10⁶ cells per ml and is delivered frozen. The cells are characterized by immunofluorescent method with antibodies of myosin and/or actinin. Cells are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded on a multiwell culture plate ready for experiments (or cells may be expanded for 2 passages at a split ratio of 1:2) under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells is not recommended.

Suggested Medium

Skeletal Muscle Cell Medium /w Kit – 500 ML

Catalog No: M1261

(500 ml Basal medium with M1261-Kit)

Laboratory Applications

Cynomolgus Monkey Primary Skeletal Muscle Cells can be used in assays of standard biochemical procedures performed with cell cultures include RT-PCR, Western blotting, immunoprecipitation, immunofluorescent flow cytometry, or generating cell derivatives for desired research applications.

Storage

Cell Biologics ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use. Live-cell shipment is also available on request. Primary Cells can never be kept at -20 °C.

Documents

MK-6167

Cell-Culture-Protocol-CB

1708462493

Disclaimer

Appropriate safety procedures should always be used with this material. Although Monkey Primary Skeletal Muscle Cells are isolated from normal monkey tissue, investigators should handle the cells that they receive from Cell Biologics with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents. The entire text of discussing Biosafety in Microbiological and Biomedical Laboratories, 5th ed. is available online at http://www.cdc.gov/biosafety/publicati ons/bmbl5/index.htm.

Publication

Viruses. 2023 Nov; 15(11): 2255.