{"product_id":"cynomolgus-monkey-primary-skeletal-muscle-cells","title":"Cynomolgus Monkey Primary Skeletal Muscle Cells","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eCynomolgus Monkey Primary Skeletal Muscle Cells from \u003ci\u003eCell Biologics\u003c\/i\u003e are isolated from Cynomolgus Monkey skeletal muscle tissue. Cynomolgus Monkey Primary Skeletal Muscle Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based coating solution for 2 min and incubated in \u003ci\u003eCell Biologics\u003c\/i\u003e Culture Complete Growth Medium generally for 3-7 days. Prior to shipping, cells at passage 2-3 are detached from the culture flasks and immediately cryo-preserved in vials. Each vial contains at least 0.5x10\u003csup\u003e6\u003c\/sup\u003e cells per ml and is delivered frozen. The cells are characterized by immunofluorescent method with antibodies of myosin and\/or actinin. Cells are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded on a multiwell culture plate ready for experiments (or cells may be expanded for 2 passages at a split ratio of 1:2) under the cell culture conditions specified by \u003ci\u003eCell Biologics\u003c\/i\u003e. Repeated freezing and thawing of cells is not recommended.\u003c\/p\u003e\n\u003ch3\u003eSuggested Medium\u003c\/h3\u003e\n\u003cp\u003e\u003cstrong\u003eSkeletal Muscle Cell Medium \/w Kit – 500 ML\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eCatalog No: M1261\u003c\/p\u003e\n\u003cp\u003e(500 ml Basal medium with M1261-Kit)\u003c\/p\u003e\n\u003ch2\u003eLaboratory Applications\u003c\/h2\u003e\n\u003cp\u003eCynomolgus Monkey Primary Skeletal Muscle Cells can be used in assays of standard biochemical procedures performed with cell cultures include RT-PCR, Western blotting, immunoprecipitation, immunofluorescent flow cytometry, or generating cell derivatives for desired research applications.\u003c\/p\u003e\n\u003ch2\u003eStorage\u003c\/h2\u003e\n\u003cp\u003e\u003ci\u003eCell Biologics\u003c\/i\u003e ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use. Live-cell shipment is also available on request. Primary Cells can never be kept at -20 °C.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/files.biofargo.com\/cell-biologics\/pdfs\/MK-6167_MK-6167.pdf\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eMK-6167\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/files.biofargo.com\/cell-biologics\/pdfs\/MK-6167_Cell-Culture-Protocol-CB.pdf\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eCell-Culture-Protocol-CB\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/files.biofargo.com\/cell-biologics\/pdfs\/MK-6167_1708462493.pdf\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003e1708462493\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003ch2\u003eDisclaimer\u003c\/h2\u003e\n\u003cp\u003eAppropriate safety procedures should always be used with this material. Although Monkey Primary Skeletal Muscle Cells are isolated from normal monkey tissue, investigators should handle the cells that they receive from \u003ci\u003eCell Biologics\u003c\/i\u003e with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents. The entire text of discussing Biosafety in Microbiological and Biomedical Laboratories, 5th ed. is available online at \u003ca href=\"http:\/\/www.cdc.gov\/biosafety\/publicati\"\u003ehttp:\/\/www.cdc.gov\/biosafety\/publicati\u003c\/a\u003e\u003ca href=\"http:\/\/www.cdc.gov\/biosafety\/publications\/bmbl5\/index.htm\"\u003eons\/bmbl5\/index.htm\u003c\/a\u003e.\u003c\/p\u003e\n\u003ch2\u003ePublication\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC10674321\/#\"\u003e\u003cstrong\u003eViruses. 2023 Nov; 15(11): 2255.\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n","brand":"Cell Biologics","offers":[{"title":"Frozen Vail","offer_id":52481726972085,"sku":"MK-6167","price":710.0,"currency_code":"USD","in_stock":true},{"title":"T25 Flask","offer_id":52481727004853,"sku":"MK-6167-1","price":760.0,"currency_code":"USD","in_stock":true},{"title":"T75 Flask","offer_id":52481727037621,"sku":"MK-6167-2","price":980.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/SmoothMuscleCellsRat-2b-500x500_73c028a7-61b6-4730-b365-44dd7c594320.jpg?v=1778252998","url":"https:\/\/biofargo.com\/products\/cynomolgus-monkey-primary-skeletal-muscle-cells","provider":"Biofargo","version":"1.0","type":"link"}