{"product_id":"cynomolgus-monkey-primary-liver-sinusoidal-endothelial-cells","title":"Cynomolgus Monkey Primary Liver Sinusoidal Endothelial Cells","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eMonkey Primary Liver Sinusoidal Endothelial Cells from Cell Biologics are isolated from Cynomolgus Monkey liver tissue and grown in gelatin pre-coated tissue culture flask with Cell Biologics’ Complete Growth Medium. Cells at passage 3 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x10\u003csup\u003e6\u003c\/sup\u003e cells per ml and is delivered frozen. Monkey Primary Liver Sinusoidal Endothelial are characterized by immunofluorescence staining with antibodies of CD31 (Catalog No. ab9498, Abcam), VE-cadherin (Catalog No. sc-28644, Santa Cruz, or Catalog No. ALX-210-232-C100, Enzo Life Sciences, Inc.), or use of fluorescence-labeled acetylated low-density lipoprotein (DiI-Ac-LDL) uptake (Catalog No. L-35353, Invitrogen), a functional marker for endothelial cells. These cells are negative for bacteria, yeast, fungi, and mycoplasma and can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells is not recommended. Monkey Primary Liver Sinusoidal Endothelial Cells can be used for cell-based adhesion, migration, vascular tube formation, transendothelial resistance (TER), western blot, immunoprecipitation, immunofluorescence flow cytometry, polymerase chain reaction, and generating cell derivatives for desired research applications.\u003c\/p\u003e\n\u003ch3\u003eSuggested Medium\u003c\/h3\u003e\n\u003cp\u003eMK1168  Complete Monkey Endothelial Cell Medium – 125 ml\u003c\/p\u003e\n\u003ch2\u003eStorage\u003c\/h2\u003e\n\u003cp\u003eCryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/files.biofargo.com\/cell-biologics\/pdfs\/MK-6017_MK-6017.pdf\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eMK-6017\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/files.biofargo.com\/cell-biologics\/pdfs\/MK-6017_Cell-Culture-Protocol-CB.pdf\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eCell-Culture-Protocol-CB\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003ch2\u003eDisclaimer\u003c\/h2\u003e\n\u003cp\u003eInvestigators should handle the cells with caution and treat all primary cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents. The entire text of discussing Biosafety in Microbiological and Biomedical Laboratories, 5th ed. is available online at\u003c\/p\u003e\n\u003cp\u003ehttp:\/\/www.cdc.gov\/biosafety\/publications\/bmbl5\/index.htm.\u003c\/p\u003e\n","brand":"Cell Biologics","offers":[{"title":"Frozen Vial","offer_id":52481678606517,"sku":"MK-6017","price":860.0,"currency_code":"USD","in_stock":true},{"title":"T25 Flask","offer_id":52481678639285,"sku":"MK-6017-1","price":910.0,"currency_code":"USD","in_stock":true},{"title":"T75 Flask","offer_id":52481678672053,"sku":"MK-6017-2","price":980.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/EndothelialCell.VWF-500x500.jpg?v=1778272653","url":"https:\/\/biofargo.com\/products\/cynomolgus-monkey-primary-liver-sinusoidal-endothelial-cells","provider":"Biofargo","version":"1.0","type":"link"}