Background

CXCL10 (also known as IP-10) is a member of the CXC chemokine family which binds to the CXCR3 receptor to exert its biological effects. CXCL10 is a 12-kDa protein and constitutes two internal disulfide cross bridges. The predicted signal peptidase cleavage generates a 10-kDa secreted polypeptide with four conserved cysteine residues in the N-terminal. The CXCL10 gene localizes on chromosome 4 at band q21, a locus associated with an acute monocytic/B-lymphocyte lineage leukemia exhibiting translocation of t (4; 11) (q21; q23). CXCL10 mediates leukocyte trafficking, adaptive immunity, inflammation, haematopoiesis and angiogenesis. Under proinflammatory conditions CXCL10 is secreted from a variety of cells, such as leukocytes, activated neutrophils, eosinophils, monocytes, epithelial cells, endothelial cells, stromal cells (fibroblasts) and keratinocytes in response to IFN-γ.

Description

The CXCL10 / IP-10 Rabbit Polyclonal Antibody is a high-quality primary antibody developed for the detection of CXCL10 (C-X-C motif chemokine 10), also known as Interferon-gamma-induced protein 10 (IP-10).
CXCL10 is a secreted chemokine involved in immune cell trafficking, inflammation, and interferon-mediated immune responses.

This antibody is generated in rabbit and supplied as an unconjugated polyclonal IgG, offering high sensitivity and broad epitope recognition. It is suitable for multiple immunoassays when used with appropriate anti-rabbit secondary antibodies.

Each lot is produced under strict quality control to ensure reliability, reproducibility, and consistent performance in research applications.

For Research Use Only (RUO). Not for diagnostic or therapeutic use.

Specifications

Reactivity	Human, Mouse, Rat
Application	WB (Western Blot), IHC (Immunohistochemistry), IF (Immunofluorescence)
Host	Rabbit
Isotype	IgG
Clonality	Polyclonal
Target	CXCL10 / IP-10
Immunogen	Recombinant human CXCL10 protein
Purification	Antigen affinity purification
Conjugation	Unconjugated
Form	Liquid
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3
Calculated Molecular Weight	11 kDa
Observed Molecular Weight	11 kDa
Gene Name	CXCL10
Full Name	Chemokine (C-X-C motif) ligand 10
Alternative Names	CXCL10; CXCL10/IP-10; IP-10; 10 kDa interferon gamma-induced protein; C-X-C motif chemokine 10
Gene ID (NCBI)	3627
GenBank Protein Accession	BC010954
UniProt ID	P02778
RRID	AB_2088002

Features

Primary Antibody (Polyclonal)

Directly recognizes CXCL10 / IP-10, requiring a secondary antibody for signal detection.
Rabbit Host, Broad Epitope Recognition

Polyclonal format enhances sensitivity and robustness across multiple assay conditions.
Unconjugated Format

Compatible with a wide range of enzyme- or fluorophore-conjugated secondary antibodies.
High Specificity for CXCL10 (IP-10)

Targets a key chemokine involved in immune regulation and inflammatory signaling.
Validated for Multiple Applications

Suitable for common immunodetection workflows.
Consistent Quality

Manufactured with lot-to-lot consistency to support reproducible research results.

Application

Application	Dilution Ratio
WB	1:500 - 1:2000
IHC	1:200 - 1:800
IF	1:100 - 1:200

Western Blot (WB)

Detection of CXCL10 protein expression in cell and tissue lysates.
Immunohistochemistry (IHC)

Localization of CXCL10 in tissue sections when used with appropriate secondary antibodies.
Immunofluorescence (IF)

Visualization of CXCL10 expression using fluorophore-conjugated anti-rabbit secondary antibodies.
ELISA

Detection and quantification of CXCL10 in biological samples (assay-dependent).
Inflammation & Immunology Research

Studies involving immune cell recruitment, cytokine signaling, infection, and autoimmune responses.

Data

Untreated, IFN gamma, LPS and Brefeldin A treated THP-1 cells were subjected to SDS PAGE followed by western blot with CXCL10/IP-10 Rabbit Polyclonal Antibody.

Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using CXCL10/IP-10 Rabbit Polyclonal Antibody.

Immunofluorescent staining of spinal cord tissues from mice 8 weeks post-SCI with CXCL10/IP-10 Rabbit Polyclonal Antibody.

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.