Introduction

Hexadecyltrimethylammonium Bromide (CTAB, CAS 57-09-0) is a high-purity cationic surfactant widely used in molecular biology, nanomaterials, and colloid chemistry. In life science research, CTAB DNA extraction is a classic method for isolating high-quality plant genomic DNA, effectively removing polysaccharides and polyphenols. In nanotechnology, CTAB is essential for gold nanorod and mesoporous silica synthesis, serving as a template and stabilizer. Biofargo offers ≥99% purity CTAB (molecular biology grade) in 100g and 500g packages, ensuring reliable performance, batch-to-batch consistency, and global compliance (COA and SDS available). As a trusted CTAB supplier in the USA, we provide fast shipping and dependable quality for laboratories worldwide.

Specification

Grade	High Purity Grade
Formula	C19H42NBr
MW	364.09
CAS	57-09-0
Appearance	White to almost white powder
Color (Photoelectric colorimetry)	≤100 Hazen
Moisture (K.F.)	≤0.8%
Assay (Titration)	≥99.0%
Storage	Store at room temperature. Protect from moisture

Chemical & Regulatory Information

UNSPSC Code: 12161902
UNSPSC Category: Detergents / Surfactants
Hazard Class: Class 9 (Miscellaneous Dangerous Substances)
UN Number: UN377
Packing Group (PG): III
DG Classification: Yes (Dangerous Goods)

Solubility

Water: Soluble (~13 g/L at 20°C)
Methanol: Freely soluble
Acetone: Slightly soluble
Benzene & Diethyl Ether: Insoluble

Applications

DNA & RNA Extraction: CTAB is widely used in plant genomic DNA isolation protocols, particularly effective in removing polysaccharides.
Nanoparticle Synthesis: Used as a template and stabilizer for mesoporous silica materials and gold nanorods.
Cationic Surfactant: Common in colloid chemistry and micelle formation.
Phase-Transfer Catalyst: Facilitates organic reactions involving immiscible reactants.
Personal Care: An active ingredient in antiseptic formulations and conditioners (as cetrimide derivatives).

Safety & Handling

Handle in a well-ventilated area with appropriate PPE (gloves, goggles, lab coat).
Avoid contact with skin, eyes, and clothing.
Refer to the Safety Data Sheet (SDS) for full hazard, storage, and disposal information.

Storage Conditions

Store at room temperature in a tightly sealed container.
Hygroscopic – recommended to store under inert gas (e.g., nitrogen) to prevent moisture uptake.
Keep away from heat and light.

Frequently Asked Questions (FAQ)

Q1: What is the main application of CTAB in molecular biology?
A1: CTAB is widely used in plant genomic DNA extraction, especially for tissues rich in polysaccharides and polyphenols. It is also used as a surfactant, nanoparticle synthesis template, and phase-transfer catalyst in chemistry and materials science.

Q2: How do you ensure the quality of your CTAB?
A2: Our CTAB is supplied with ≥99% purity, low moisture content (≤0.8%), and a clear white appearance. Each batch is strictly quality-controlled to ensure consistency and reliability for sensitive applications like DNA extraction and nanoparticle synthesis.

Q3: CTAB is hygroscopic—will it form clumps during storage?
A3: CTAB is prone to moisture uptake. To prevent clumping, it should be stored in a tightly sealed container at room temperature, ideally under an inert gas (nitrogen or argon) to minimize humidity exposure.

Q4: Is CTAB safe to handle in the lab?
A4: CTAB is classified as a Class 9 Dangerous Substance (UN377). It may cause irritation to skin, eyes, and respiratory tract. Always handle it in a well-ventilated area with proper PPE (gloves, goggles, lab coat) and consult the Safety Data Sheet (SDS) for detailed instructions.

Q5: Can residual CTAB affect downstream molecular biology experiments?
A5: Yes. If CTAB is not completely removed during DNA extraction, it may inhibit PCR and enzymatic reactions. Using high-purity CTAB and optimizing the washing steps minimizes this risk.

Q6: What do researchers care about most when purchasing CTAB?
A6: Researchers are most concerned about purity, batch-to-batch consistency, storage stability, and safety compliance. Reliable supply and technical documentation (COA, SDS) are also critical.

Documents

COA

SDS

Disclaimer: For laboratory research use only.

Hexadecyltrimethylammonium bromide(CTAB)-99%-CAS 57-09-0