Specification

Grade	Indicator
Formula	C25H30N3Cl
MW	407.99
CAS	548-62-9
Appearance	Dark green or dark brown crystals
Absorption test	PASS
Non-aqueous titration	PASS
Ethanol dissolution test	PASS
Loss on drying	≤6.0%
Residue on ignition	≤0.50%
Organic impurities	PASS
Storage	Store at room temperature. Protect from moisture.

Application

Crystal violet is used as an active component, primary stain, of Gram stain for differentiation of Gram-negative versus Gram-positive bacteria.
It has been used to check cell viability. It has also been used for the staining of cells to study cell migration and invasion.

Biochem/physiol Actions

Crystal violet can be used for DNA visualization in agarose gels. The dye is used only in the presence of high concentrations of DNA. Crystal violet is also used for the staining of bacteria in gram staining technique. It is also used for the staining of plant chromosomes. Crystal Violet also helps in colorimetric measurement of cell viability.

Documents

COA

SDS

Disclaimer: For laboratory research use only.