{"product_id":"crithidia-fasciculata-probe-qpcr-kit","title":"Crithidia fasciculata Probe qPCR Kit","description":"\u003cstyle\u003e\n  .biofargo-product {\n    max-width: 1000px;\n    margin: 0 auto;\n    padding: 0;\n    color: #333333;\n    font-family: Arial, Helvetica, sans-serif;\n    font-size: 16px;\n    line-height: 1.65;\n  }\n\n  .biofargo-product,\n  .biofargo-product * {\n    box-sizing: border-box;\n  }\n\n  .biofargo-product section {\n    margin: 0 0 32px;\n  }\n\n  .biofargo-product h2 {\n    margin: 0 0 16px;\n    padding-bottom: 9px;\n    border-bottom: 2px solid #168bc4;\n    color: #055da9;\n    font-size: 24px;\n    line-height: 1.3;\n  }\n\n  .biofargo-product p {\n    margin: 0 0 13px;\n  }\n\n  .biofargo-product ul {\n    margin: 0;\n    padding-left: 23px;\n  }\n\n  .biofargo-product li {\n    margin-bottom: 7px;\n  }\n\n  .biofargo-table-wrap {\n    width: 100%;\n    overflow-x: auto;\n    -webkit-overflow-scrolling: touch;\n  }\n\n  .biofargo-table {\n    width: 100%;\n    min-width: 650px;\n    border-collapse: collapse;\n    border-spacing: 0;\n    background: #ffffff;\n  }\n\n  .biofargo-table th,\n  .biofargo-table td {\n    padding: 12px 14px;\n    border: 1px solid #d9e1e7;\n    text-align: left;\n    vertical-align: middle;\n  }\n\n  .biofargo-table th {\n    width: 34%;\n    background: #eaf5fa;\n    color: #174f72;\n    font-weight: 700;\n  }\n\n  .biofargo-components th {\n    width: auto;\n    background: #168bc4;\n    color: #ffffff;\n    text-align: center;\n  }\n\n  .biofargo-components td {\n    text-align: center;\n  }\n\n  .biofargo-components tr:nth-child(even) td {\n    background: #f5fafc;\n  }\n\n  .biofargo-note {\n    margin-top: 15px;\n    padding: 13px 15px;\n    border: 1px solid #d8e8f0;\n    border-radius: 4px;\n    background: #f2f8fb;\n    color: #333333;\n  }\n\n  .biofargo-faq {\n    background: #ffffff;\n  }\n\n  .biofargo-faq-item {\n    margin: 0;\n    border-bottom: 1px solid #d9e1e7;\n  }\n\n  .biofargo-faq-item:first-child {\n    border-top: 1px solid #d9e1e7;\n  }\n\n  .biofargo-faq-question {\n    display: flex;\n    width: 100%;\n    align-items: center;\n    justify-content: space-between;\n    gap: 16px;\n    padding: 18px 4px;\n    border: 0;\n    background: transparent;\n    color: #174f72;\n    font-family: Arial, Helvetica, sans-serif;\n    font-size: 16px;\n    font-weight: 700;\n    line-height: 1.4;\n    text-align: left;\n    cursor: pointer;\n  }\n\n  .biofargo-faq-question:hover {\n    color: #168bc4;\n  }\n\n  .biofargo-faq-icon {\n    position: relative;\n    width: 18px;\n    height: 18px;\n    flex: 0 0 18px;\n    color: #168bc4;\n  }\n\n  .biofargo-faq-icon::before,\n  .biofargo-faq-icon::after {\n    content: \"\";\n    position: absolute;\n    background: currentColor;\n    border-radius: 2px;\n  }\n\n  .biofargo-faq-icon::before {\n    top: 8px;\n    left: 0;\n    width: 18px;\n    height: 2px;\n  }\n\n  .biofargo-faq-icon::after {\n    top: 0;\n    left: 8px;\n    width: 2px;\n    height: 18px;\n    transition: opacity 0.2s ease;\n  }\n\n  .biofargo-faq-item.is-open .biofargo-faq-icon::after {\n    opacity: 0;\n  }\n\n  .biofargo-faq-answer {\n    display: none;\n    max-width: 880px;\n    padding: 0 4px 18px;\n    color: #444444;\n  }\n\n  .biofargo-faq-answer.is-open {\n    display: block;\n  }\n\n  .biofargo-faq-answer p {\n    margin: 0;\n  }\n\n  .biofargo-ruo {\n    margin: 28px 0 0;\n    padding: 13px 15px;\n    border-radius: 4px;\n    background: #eaf5fa;\n    color: #174f72;\n    font-size: 14px;\n    font-weight: 600;\n    text-align: center;\n  }\n\n  @media (max-width: 767px) {\n    .biofargo-product {\n      font-size: 15px;\n    }\n\n    .biofargo-product h2 {\n      font-size: 21px;\n    }\n\n    .biofargo-table th,\n    .biofargo-table td {\n      padding: 10px 12px;\n    }\n\n    .biofargo-faq-question {\n      padding: 16px 3px;\n      font-size: 15px;\n    }\n  }\n\u003c\/style\u003e\n\u003cdiv class=\"biofargo-product\"\u003e\n\u003csection id=\"description\"\u003e\n\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eThe \u003cem\u003eCrithidia fasciculata\u003c\/em\u003e Probe qPCR Kit is designed for the qualitative detection of \u003cem\u003eCrithidia fasciculata\u003c\/em\u003e DNA using probe-based real-time PCR. Purified DNA is amplified with target-specific primers and a fluorescent probe, enabling real-time monitoring of the amplification reaction.\u003c\/p\u003e\n\u003cp\u003eThis kit provides researchers with a targeted molecular method for detecting \u003cem\u003eC. fasciculata\u003c\/em\u003e DNA in prepared research samples. It may support laboratory studies involving kinetoplastid biology, cultured organisms, kinetoplast DNA, and insect–protozoan interactions.\u003c\/p\u003e\n\u003cdiv class=\"biofargo-note\"\u003e\n\u003cstrong\u003eNote:\u003c\/strong\u003e This product is supplied without an internal control. Sample compatibility and analytical performance depend on the validated nucleic acid extraction and testing workflow.\u003c\/div\u003e\n\u003c\/section\u003e\n\u003csection id=\"specification\"\u003e\n\u003ch2\u003eSpecification\u003c\/h2\u003e\n\u003cdiv class=\"biofargo-table-wrap\"\u003e\n\u003ctable class=\"biofargo-table\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth\u003eProduct Name\u003c\/th\u003e\n\u003ctd\u003e\n\u003cem\u003eCrithidia fasciculata\u003c\/em\u003e Probe qPCR Kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eTarget Organism\u003c\/th\u003e\n\u003ctd\u003e\n\u003cem\u003eCrithidia fasciculata\u003c\/em\u003e Léger\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eTarget Material\u003c\/th\u003e\n\u003ctd\u003eExtracted DNA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eDetection Technology\u003c\/th\u003e\n\u003ctd\u003eProbe-based real-time PCR\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eDetection Type\u003c\/th\u003e\n\u003ctd\u003eQualitative detection\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eInternal Control\u003c\/th\u003e\n\u003ctd\u003eNot included\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eNCBI Taxonomy ID\u003c\/th\u003e\n\u003ctd\u003e5656\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eIntended Use\u003c\/th\u003e\n\u003ctd\u003eLaboratory research use only\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003csection id=\"features\"\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eDesigned for qualitative detection of \u003cem\u003eCrithidia fasciculata\u003c\/em\u003e DNA.\u003c\/li\u003e\n\u003cli\u003eUses target-specific primer and probe chemistry.\u003c\/li\u003e\n\u003cli\u003eEnables real-time fluorescence monitoring during amplification.\u003c\/li\u003e\n\u003cli\u003eSuitable for purified DNA prepared from research samples.\u003c\/li\u003e\n\u003cli\u003eIncludes a non-infectious plasmid-based positive control for assay verification.\u003c\/li\u003e\n\u003cli\u003eIntended exclusively for laboratory research applications.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/section\u003e\n\u003csection id=\"kit-components\"\u003e\n\u003ch2\u003eKit Components\u003c\/h2\u003e\n\u003cdiv class=\"biofargo-table-wrap\"\u003e\n\u003ctable class=\"biofargo-table biofargo-components\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003eComponent\u003c\/th\u003e\n\u003cth\u003eDescription\u003c\/th\u003e\n\u003cth\u003eSize\u003c\/th\u003e\n\u003cth\u003eVial\u003c\/th\u003e\n\u003cth\u003eLid Color\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e2× Probe qPCR Master Mix\u003c\/td\u003e\n\u003ctd\u003eOptimized buffer and enzyme mixture\u003c\/td\u003e\n\u003ctd\u003e500 µL\u003c\/td\u003e\n\u003ctd\u003e0.5 mL vial\u003c\/td\u003e\n\u003ctd\u003eBlue\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ePrimer-Probe Powder\u003c\/td\u003e\n\u003ctd\u003eLyophilized target-specific primer and probe set\u003c\/td\u003e\n\u003ctd\u003e50 reactions\u003c\/td\u003e\n\u003ctd\u003e1.5 mL vial\u003c\/td\u003e\n\u003ctd\u003eBrown\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ePositive Control\u003c\/td\u003e\n\u003ctd\u003eNon-infectious plasmid-based control\u003c\/td\u003e\n\u003ctd\u003e0.5 mL\u003c\/td\u003e\n\u003ctd\u003e0.5 mL vial\u003c\/td\u003e\n\u003ctd\u003eYellow\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eNuclease-Free Water\u003c\/td\u003e\n\u003ctd\u003eFor reaction preparation and dilution\u003c\/td\u003e\n\u003ctd\u003e1 mL\u003c\/td\u003e\n\u003ctd\u003e1.5 mL vial\u003c\/td\u003e\n\u003ctd\u003eGreen\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eUser Manual\u003c\/td\u003e\n\u003ctd\u003eProduct instructions\u003c\/td\u003e\n\u003ctd\u003e1 set\u003c\/td\u003e\n\u003ctd\u003e—\u003c\/td\u003e\n\u003ctd\u003e—\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003csection id=\"application\"\u003e\n\u003ch2\u003eApplication\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eQualitative detection of \u003cem\u003eCrithidia fasciculata\u003c\/em\u003e DNA.\u003c\/li\u003e\n\u003cli\u003eMolecular confirmation of laboratory cultures.\u003c\/li\u003e\n\u003cli\u003eScreening in insect–protozoan interaction studies.\u003c\/li\u003e\n\u003cli\u003eKinetoplast and trypanosomatid molecular biology research.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cdiv class=\"biofargo-note\"\u003eUse extracted DNA of suitable purity. The applicable sample types should be determined according to the validated extraction method and laboratory workflow.\u003c\/div\u003e\n\u003c\/section\u003e\n\u003csection id=\"storage\"\u003e\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eStore the kit at −20°C.\u003c\/li\u003e\n\u003cli\u003eShip under dry-ice conditions.\u003c\/li\u003e\n\u003cli\u003eAvoid repeated freeze–thaw cycles.\u003c\/li\u003e\n\u003cli\u003eProtect fluorescent probe components from prolonged light exposure.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/section\u003e\n\u003csection id=\"faq\"\u003e\n\u003ch2\u003eFrequently Asked Questions\u003c\/h2\u003e\n\u003cdiv class=\"biofargo-faq\"\u003e\n\u003cdiv class=\"biofargo-faq-item\"\u003e\n\u003cbutton class=\"biofargo-faq-question\" type=\"button\"\u003e What does the Crithidia fasciculata Probe qPCR Kit detect? \u003cspan class=\"biofargo-faq-icon\"\u003e\u003c\/span\u003e \u003c\/button\u003e\n\u003cdiv class=\"biofargo-faq-answer\"\u003e\n\u003cp\u003eThe kit is designed for the qualitative detection of \u003cem\u003eCrithidia fasciculata\u003c\/em\u003e DNA using probe-based real-time PCR.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"biofargo-faq-item\"\u003e\n\u003cbutton class=\"biofargo-faq-question\" type=\"button\"\u003e Is an internal control included? \u003cspan class=\"biofargo-faq-icon\"\u003e\u003c\/span\u003e \u003c\/button\u003e\n\u003cdiv class=\"biofargo-faq-answer\"\u003e\n\u003cp\u003eNo. The standard kit is supplied without an internal control. An internal-control configuration may be requested separately.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"biofargo-faq-item\"\u003e\n\u003cbutton class=\"biofargo-faq-question\" type=\"button\"\u003e What type of sample can be tested? \u003cspan class=\"biofargo-faq-icon\"\u003e\u003c\/span\u003e \u003c\/button\u003e\n\u003cdiv class=\"biofargo-faq-answer\"\u003e\n\u003cp\u003eThe kit is intended for purified DNA prepared from research samples. Compatibility depends on the selected nucleic acid extraction method and validated workflow.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"biofargo-faq-item\"\u003e\n\u003cbutton class=\"biofargo-faq-question\" type=\"button\"\u003e Is this kit intended for clinical diagnosis? \u003cspan class=\"biofargo-faq-icon\"\u003e\u003c\/span\u003e \u003c\/button\u003e\n\u003cdiv class=\"biofargo-faq-answer\"\u003e\n\u003cp\u003eNo. This kit is intended strictly for laboratory research use and is not intended for clinical diagnostic or therapeutic applications.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003cdiv class=\"biofargo-ruo\"\u003eFor Research Use Only. Not for use in diagnostic procedures.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"Biofargo","offers":[{"title":"Default Title","offer_id":53231538733237,"sku":"BF-56560001","price":470.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/crithidia-fasciculata-probe-qpcr-kit1.jpg?v=1788849923","url":"https:\/\/biofargo.com\/products\/crithidia-fasciculata-probe-qpcr-kit","provider":"Biofargo","version":"1.0","type":"link"}