Description

Coxsackie Virus A18(Cox A18) Probe Realtime RT-PCR Kit is a one-step RT-PCR kit intended for research use in nucleic acid analysis under controlled laboratory conditions using probe-based real-time PCR. Purified RNA is reverse transcribed into cDNA and subsequently amplified using sequence-specific primers and probes. During amplification, fluorescence signals are generated through probe cleavage, allowing real-time monitoring of amplification under controlled laboratory conditions. The resulting signals may be used for comparative analysis of target nucleic acids.

Note: Supplied without internal control. Internal control version can be customized based on your assay requirements.
This product was previously listed under catalog number 15-17218 and is now updated to BF-77262060 for improved product management.

Key Features

One-step RT-PCR workflow to reduce handling steps and minimize contamination risk.
Demonstrates high sensitivity under optimized laboratory conditions.
High specificity based on primer-probe design.
Includes a non-infectious plasmid-based positive control for assay verification.
Compatible with commonly used RNA purification methods.
Suitable for research applications including assay development and method evaluation.
Broad dynamic range under laboratory conditions.
For research use only. Not intended for diagnostic or therapeutic use.

Kit Components

Component Name	Description	Size	Vial	Lid Color
2× qPCR Master Mix	Contains optimized buffer and Taq polymerase mix	500 μL	0.5 μL Vial	Natural
Primer-Probe Mix	Target-specific primer and probe set	165 μL	1.5 mL Vial	Brown
Positive Control (1E7 copies/µL)	Non-infectious plasmid-based control	50 mL	0.5 mL Vial	Yellow
Nuclease-free Water	For reaction setup and kit blank control (no-template control)	800 μL	1.5 mL Vial	Blue
Manual		1 Set	NA	NA

Application Areas

Molecular biology research: qPCR-based detection and quantification of target nucleic acids.
Assay development: Suitable for method development, optimization, and analytical performance evaluation.
Industry applications (research use): Widely used in biotechnology, pharmaceutical, academic, and agricultural research laboratories.
For Research Use Only (RUO). Not for use in diagnostic procedures.

Storage & Shipping

Store at - 20 °C
The product is shipped with dry ice to maintain low-temperature conditions and ensure product integrity during transit.

FAQ

Q: Are Biofargo PCR and qPCR Kits compatible with my thermal cycler or real-time PCR instrument?

A: Yes. Biofargo PCR Kits and probe-based qPCR Kits are designed for broad compatibility with mainstream instruments.

PCR Kits can be used with standard thermal cyclers from leading brands.

Probe-based qPCR Kits are fully compatible with major real-time PCR platforms, including ABI, Bio-Rad, Roche, QuantStudio, Qiagen Rotor-Gene Q (5-plex), and Genesig Q16.

Our technical team is available to assist with instrument setup and run optimization to ensure the best results.

Documents

Coxsackie Virus A18(Cox A18) Probe Realtime RT-PCR Kit User Manual

MSDS