Description

Coomassie Brilliant Blue Staining Kit (Staining Solution + Destaining Solution) is a classic protein gel staining kit designed for reliable visualization of proteins separated by SDS-PAGE or native PAGE electrophoresis. During routine protein analysis, unclear protein bands, high gel background, or inconsistent staining results can affect evaluation of protein expression, purification efficiency, and electrophoresis quality. This Coomassie Brilliant Blue R-250 staining solution with compatible destaining solution provides a simple and reproducible workflow for detecting protein bands after polyacrylamide gel electrophoresis.

Based on the traditional Coomassie Brilliant Blue R-250 staining method, this protein gel staining kit enables efficient binding of dye molecules to proteins while allowing removal of excess background staining through the included destaining solution. The resulting clear blue protein bands are suitable for SDS-PAGE analysis, native PAGE staining, protein purification evaluation, and post-transfer gel examination in Western blot workflows.

Coomassie Brilliant Blue Staining Kit is designed as a convenient two-component system containing ready-to-use staining and destaining solutions, helping researchers perform routine protein electrophoresis staining without preparing individual reagents. It provides an economical and dependable solution for laboratories requiring consistent protein visualization during molecular biology and biochemical research.

Specifications

Product Name	Coomassie Brilliant Blue Staining Kit (Staining Solution + Destaining Solution)
SKU	RP0091
Product Type	Protein Gel Staining Kit
Staining Dye	Coomassie Brilliant Blue R-250
Components	Coomassie Brilliant Blue Staining Solution + Destaining Solution
Package Size	100 mL Staining Solution + 500 mL Destaining Solution
Applications	SDS-PAGE staining, Native PAGE staining, Protein electrophoresis analysis, Western blot gel checking
Storage	Room temperature
Shelf Life	18 months

Features

Classic Coomassie Brilliant Blue R-250 staining: Provides a widely used protein gel staining method for routine SDS-PAGE and native PAGE analysis.
Complete staining and destaining system: Includes both protein staining solution and destaining solution for convenient gel visualization workflows.
Clear protein band visualization: Enables detection of separated protein bands with reduced background after destaining.
Compatible with common electrophoresis workflows: Suitable for polyacrylamide gel staining after protein electrophoresis experiments.
Reliable routine protein detection: Provides an economical solution for protein expression analysis, purification evaluation, and laboratory teaching applications.

Applications

Coomassie Brilliant Blue Staining Kit is widely used for protein visualization after electrophoresis and supports multiple protein analysis workflows in molecular biology and biochemical research.

SDS-PAGE Protein Gel Staining: Used to visualize protein bands after SDS-PAGE separation and evaluate protein expression patterns.
Native PAGE Gel Staining: Suitable for non-denaturing polyacrylamide gel electrophoresis where protein complexes and native protein states are analyzed.
Protein Purification Analysis: Helps evaluate purification efficiency by comparing protein profiles before and after purification steps.
Recombinant Protein Expression Evaluation: Provides visual confirmation of target protein expression in bacterial, mammalian, or other expression systems.
Western Blot Workflow Support: Used for detecting residual proteins in PAGE gels after membrane transfer to evaluate transfer efficiency.
Protein Electrophoresis Quality Control: Allows researchers to check gel separation quality, loading consistency, and overall electrophoresis performance.
Laboratory Education and Training: Suitable for teaching laboratories demonstrating protein electrophoresis and staining principles.

Protocol Overview

After completion of protein electrophoresis, the polyacrylamide gel is stained with Coomassie Brilliant Blue staining solution followed by destaining to remove excess dye and reveal protein bands.

Place the PAGE gel into sufficient Coomassie Brilliant Blue staining solution to fully cover the gel.
Incubate with gentle shaking at room temperature for approximately 1 hour or longer depending on gel thickness and staining conditions.
Remove staining solution. The staining solution may be recovered and reused several times.
Add sufficient destaining solution to completely cover the gel.
Destain with gentle shaking at room temperature for 4–24 hours, replacing destaining solution several times until background staining is removed.
Store the stained gel in water for imaging or preserve with glycerol-containing solution for longer storage.

Staining and destaining time may vary depending on gel thickness, protein amount, and experimental requirements. Over-destaining may reduce protein band intensity.

Ordering Information

SKU	Product	Size
RP0091	Coomassie Brilliant Blue Staining Kit (Staining Solution + Destaining Solution)	100 mL + 500 mL

FAQ

What is Coomassie Brilliant Blue Staining Kit used for?

Coomassie Brilliant Blue Staining Kit is used for protein gel staining after SDS-PAGE or native PAGE electrophoresis. It allows researchers to visualize protein bands and evaluate protein expression, purification, and electrophoresis results.

What proteins can be detected with Coomassie Brilliant Blue R-250 staining?

Coomassie Brilliant Blue R-250 staining can detect a broad range of proteins separated by polyacrylamide gel electrophoresis. Detection sensitivity depends on protein properties, staining conditions, and sample loading amount.

Can this Coomassie staining kit be used for SDS-PAGE?

Yes. This protein gel staining kit is specifically suitable for SDS-PAGE gel staining and is commonly used for routine protein electrophoresis analysis after separation.

How long does Coomassie Brilliant Blue staining take?

Typical staining is performed at room temperature for approximately 1 hour or longer depending on gel thickness, temperature, and protein amount. Destaining usually requires several hours until the background becomes sufficiently clear.

What is the purpose of the destaining solution?

The destaining solution removes unbound Coomassie Brilliant Blue dye from the polyacrylamide gel background while maintaining the blue signal from protein bands, improving visualization clarity.

Can the staining solution be reused?

Yes. The staining solution can typically be recovered and reused multiple times depending on staining performance and laboratory requirements.

What is the difference between Coomassie R-250 staining and silver staining?

Coomassie Brilliant Blue R-250 staining provides a simple, economical, and reproducible protein visualization method, while silver staining generally offers higher sensitivity but requires more complicated procedures.