{"product_id":"complete-fibroblast-medium-with-supplement-kit-500-ml","title":"Complete Fibroblast Medium with Supplement Kit, 500 mL","description":"\u003cdiv class=\"bf-rich\"\u003e\n\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eComplete Fibroblast Medium with Supplement Kit, 500 mL is a complete culture medium designed for the growth and proliferation of fibroblasts in vitro. It has been tested and optimized for fibroblast culture and is formulated for use with 5% CO\u003csub\u003e2\u003c\/sub\u003e and 95% air in a humidified incubator.\u003c\/p\u003e\n\u003cp\u003eThe kit contains 500 mL of basal medium — with essential and non-essential amino acids, vitamins, organic and inorganic compounds, hormones, growth factors and trace minerals — together with a supplement kit of FGF, hydrocortisone, antibiotic-antimycotic solution and fetal bovine serum. It is the suggested medium for Biofargo primary fibroblasts, including human and canine lung fibroblasts.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"bf-rich\"\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable border=\"1\" style=\"border-collapse: collapse; width: 90%;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003e\u003cstrong\u003eParameter\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\u003cstrong\u003eSpecification\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eProduct Name\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003eComplete Fibroblast Medium with Supplement Kit, 500 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eCatalog Number\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003eBF-41875525\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eFormat\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003eComplete medium kit: basal medium + supplement kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eVolume\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e500 mL basal medium\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eSupplements\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003eFGF, hydrocortisone, antibiotic-antimycotic, fetal bovine serum (FBS)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eCell Type\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003eFibroblasts\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eCulture Conditions\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e5% CO\u003csub\u003e2\u003c\/sub\u003e \/ 95% air, humidified incubator\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eIntended Use\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003eFor research use only\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"bf-rich\"\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003cp\u003eComplete, ready-to-supplement system optimized for fibroblast growth and proliferation.\u003c\/p\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cp\u003eContains FGF and hydrocortisone to support fibroblast expansion.\u003c\/p\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cp\u003eSupplied with FBS and antibiotic-antimycotic solution — no additional components required.\u003c\/p\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cp\u003eMatched to Biofargo primary fibroblasts, including fluorescent-protein-expressing and immortalized lines.\u003c\/p\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cp\u003eComplete medium stable for up to two months at 4 °C.\u003c\/p\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"bf-rich\"\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003cp\u003e\u003cstrong\u003ePrimary Fibroblast Culture:\u003c\/strong\u003e Expansion and maintenance of primary fibroblasts from lung, dermal, cardiac and other tissues.\u003c\/p\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cp\u003e\u003cstrong\u003eReporter Cell Studies:\u003c\/strong\u003e Culture of GFP-, RFP- and luciferase-expressing fibroblasts for imaging and co-culture experiments.\u003c\/p\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cp\u003e\u003cstrong\u003eFibrosis \u0026amp; Wound-Healing Research:\u003c\/strong\u003e Fibroblast proliferation, migration and extracellular matrix studies.\u003c\/p\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cp\u003e\u003cstrong\u003eCo-culture Models:\u003c\/strong\u003e Stromal support for epithelial, endothelial and tumor cell co-culture systems.\u003c\/p\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"bf-rich\"\u003e\n\u003ch2\u003eRecommended For\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003cp\u003e\u003ca href=\"https:\/\/biofargo.com\/products\/rfp-expressing-human-primary-lung-fibroblasts\"\u003eRFP-Expressing Human Primary Lung Fibroblasts\u003c\/a\u003e\u003c\/p\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cp\u003e\u003ca href=\"https:\/\/biofargo.com\/products\/immortalized-rfp-expressing-human-primary-lung-fibroblasts\"\u003eImmortalized RFP-Expressing Human Primary Lung Fibroblasts\u003c\/a\u003e\u003c\/p\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cp\u003e\u003ca href=\"https:\/\/biofargo.com\/products\/rfp-expressing-canine-primary-lung-fibroblasts\"\u003eRFP-Expressing Canine Primary Lung Fibroblasts\u003c\/a\u003e\u003c\/p\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cp\u003e\u003ca href=\"https:\/\/biofargo.com\/products\/immortalized-rfp-expressing-canine-primary-lung-fibroblasts\"\u003eImmortalized RFP-Expressing Canine Primary Lung Fibroblasts\u003c\/a\u003e\u003c\/p\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cp\u003e\u003ca href=\"https:\/\/biofargo.com\/products\/human-primary-lung-fibroblasts\"\u003eHuman Primary Lung Fibroblasts\u003c\/a\u003e and \u003ca href=\"https:\/\/biofargo.com\/products\/canine-primary-lung-fibroblasts\"\u003eCanine Primary Lung Fibroblasts\u003c\/a\u003e\u003c\/p\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"bf-rich\"\u003e\n\u003ch2\u003eKit Components\u003c\/h2\u003e\n\u003ctable border=\"1\" style=\"border-collapse: collapse; width: 90%;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003e\u003cstrong\u003eComponent\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\u003cstrong\u003eVolume\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eFibroblast Basal Medium\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e500 mL\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e2–8 °C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eFGF\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e0.5 mL\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e−20 °C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eHydrocortisone\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e0.5 mL\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e−20 °C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eAntibiotic-Antimycotic Solution\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e5.0 mL\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e−20 °C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eFetal Bovine Serum (FBS)\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e25.0 mL\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e−20 °C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"bf-rich\"\u003e\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003cp\u003eStore the basal medium at 2–8 °C.\u003c\/p\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cp\u003eStore FGF, hydrocortisone, FBS and antibiotic-antimycotic solution at −20 °C.\u003c\/p\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cp\u003eComplete medium (after supplementation): store at 4 °C for up to two months. Protect from light.\u003c\/p\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cp\u003eShipped with ice packs and dry ice.\u003c\/p\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003cstyle\u003e\n  .bf-rich .josh-faq { background: #fff; color: #000; font-family: \"IBM Plex Sans\", sans-serif; }\n  .bf-rich .josh-faq__inner { padding: 30px 300px 60px 30px; }\n  .bf-rich .josh-faq__item { border-bottom: 1px solid #e5e7eb; padding: 0; }\n  .bf-rich .josh-faq__item:first-child { border-top: 1px solid #e5e7eb; }\n  .bf-rich .josh-faq__question { width: 100%; margin: 0; padding: 20px 0; display: flex; align-items: center; justify-content: space-between; gap: 16px; color: #505052; background: none; border: 0; font: 700 16px\/1.4 \"IBM Plex Sans\", sans-serif; text-align: left; cursor: pointer; list-style: none; }\n  .bf-rich .josh-faq__question::-webkit-details-marker { display: none; }\n  .bf-rich .josh-faq__question:hover { color: #505052; }\n  .bf-rich .josh-faq__icon { position: relative; width: 20px; height: 20px; flex: 0 0 20px; transition: transform .25s ease; }\n  .bf-rich .josh-faq__icon::before, .bf-rich .josh-faq__icon::after { position: absolute; content: \"\"; background: currentColor; border-radius: 2px; transition: opacity .25s ease, transform .25s ease; }\n  .bf-rich .josh-faq__icon::before { top: 50%; left: 0; width: 100%; height: 2px; transform: translateY(-50%); }\n  .bf-rich .josh-faq__icon::after { top: 0; left: 50%; width: 2px; height: 100%; transform: translateX(-50%); }\n  .bf-rich .josh-faq__item[open] .josh-faq__icon::after { opacity: 0; transform: translateX(-50%) rotate(90deg); }\n  .bf-rich .josh-faq__answer { max-width: 860px; padding: 0 0 20px; color: #505052; font: 400 16px\/1.6 \"IBM Plex Sans\", sans-serif; }\n  .bf-rich .josh-faq__answer p { margin: 0 0 15px; color: #505052; font: inherit; }\n  .bf-rich .josh-faq__answer p:last-child { margin-bottom: 0; }\n  .bf-rich .josh-faq__answer ul { margin: 8px 0 12px; padding-left: 22px; }\n  .bf-rich .josh-faq__answer li { margin-bottom: 6px; font: inherit; list-style-type: disc; }\n  @media (max-width: 767px) { .bf-rich .josh-faq__inner { padding: 24px 0 40px; } .bf-rich .josh-faq__question { padding: 16px 0; font-size: 15px; } .bf-rich .josh-faq__answer { font-size: 15px; } }\n\u003c\/style\u003e\n\u003cdiv class=\"bf-rich\"\u003e\n\u003ch2\u003eFrequently Asked Questions (FAQs)\u003c\/h2\u003e\n\u003cp\u003eCommon questions about Complete Fibroblast Medium with Supplement Kit, 500 mL.\u003c\/p\u003e\n\u003cdiv class=\"josh-faq\"\u003e\u003csection class=\"josh-faq__inner\"\u003e\u003cdiv class=\"josh-faq__list\"\u003e\n\u003cdetails class=\"josh-faq__item\"\u003e\u003csummary class=\"josh-faq__question\"\u003eWhich cells is this medium suitable for?\u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\u003c\/summary\u003e\u003cdiv class=\"josh-faq__answer\"\u003e\u003cp\u003eIt is designed and optimized for the culture of fibroblasts, and is the suggested medium for Biofargo primary fibroblasts, including human and canine lung fibroblasts and their GFP-, RFP- and luciferase-expressing and immortalized versions.\u003c\/p\u003e\u003c\/div\u003e\u003c\/details\u003e\n\u003cdetails class=\"josh-faq__item\"\u003e\u003csummary class=\"josh-faq__question\"\u003eHow do I prepare the complete medium?\u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\u003c\/summary\u003e\u003cdiv class=\"josh-faq__answer\"\u003e\u003cp\u003eThaw the supplement components, add the entire supplement kit to the 500 mL basal medium under sterile conditions, and mix gently. Label the bottle with the preparation date.\u003c\/p\u003e\u003c\/div\u003e\u003c\/details\u003e\n\u003cdetails class=\"josh-faq__item\"\u003e\u003csummary class=\"josh-faq__question\"\u003eHow long is the complete medium stable?\u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\u003c\/summary\u003e\u003cdiv class=\"josh-faq__answer\"\u003e\u003cp\u003eAfter supplementation, the complete medium can be stored at 4 °C, protected from light, for up to two months. To assure sterility after two weeks, or if sterility may have been compromised during supplementation, the medium can be refiltered through a 0.2 µm filter.\u003c\/p\u003e\u003c\/div\u003e\u003c\/details\u003e\n\u003cdetails class=\"josh-faq__item\"\u003e\u003csummary class=\"josh-faq__question\"\u003eDo fibroblasts need a coated culture vessel?\u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\u003c\/summary\u003e\u003cdiv class=\"josh-faq__answer\"\u003e\u003cp\u003eBiofargo primary fibroblasts are expanded in gelatin pre-coated tissue culture flasks. Pre-coating with a 0.1% gelatin solution is recommended for best attachment.\u003c\/p\u003e\u003c\/div\u003e\u003c\/details\u003e\n\u003cdetails class=\"josh-faq__item\"\u003e\u003csummary class=\"josh-faq__question\"\u003eIs a phenol red-free or serum-free version available?\u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\u003c\/summary\u003e\u003cdiv class=\"josh-faq__answer\"\u003e\u003cp\u003eYes. Phenol red-free and serum-free fibroblast medium formats, as well as the basal medium alone, are available on request. Please contact contact@biofargo.com for a quote.\u003c\/p\u003e\u003c\/div\u003e\u003c\/details\u003e\n\u003cdetails class=\"josh-faq__item\"\u003e\u003csummary class=\"josh-faq__question\"\u003eCan it be used for clinical or diagnostic purposes?\u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\u003c\/summary\u003e\u003cdiv class=\"josh-faq__answer\"\u003e\u003cp\u003eNo. This product is for research use only and is not intended for human, diagnostic or therapeutic use.\u003c\/p\u003e\u003c\/div\u003e\u003c\/details\u003e\n\u003c\/div\u003e\u003c\/section\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cscript type=\"application\/ld+json\"\u003e{\"@context\": \"https:\/\/schema.org\", \"@type\": \"FAQPage\", \"mainEntity\": [{\"@type\": \"Question\", \"name\": \"Which cells is this medium suitable for?\", \"acceptedAnswer\": {\"@type\": \"Answer\", \"text\": \"It is designed and optimized for the culture of fibroblasts, and is the suggested medium for Biofargo primary fibroblasts, including human and canine lung fibroblasts and their GFP-, RFP- and luciferase-expressing and immortalized versions.\"}}, {\"@type\": \"Question\", \"name\": \"How do I prepare the complete medium?\", \"acceptedAnswer\": {\"@type\": \"Answer\", \"text\": \"Thaw the supplement components, add the entire supplement kit to the 500 mL basal medium under sterile conditions, and mix gently. Label the bottle with the preparation date.\"}}, {\"@type\": \"Question\", \"name\": \"How long is the complete medium stable?\", \"acceptedAnswer\": {\"@type\": \"Answer\", \"text\": \"After supplementation, the complete medium can be stored at 4 °C, protected from light, for up to two months. To assure sterility after two weeks, or if sterility may have been compromised during supplementation, the medium can be refiltered through a 0.2 µm filter.\"}}, {\"@type\": \"Question\", \"name\": \"Do fibroblasts need a coated culture vessel?\", \"acceptedAnswer\": {\"@type\": \"Answer\", \"text\": \"Biofargo primary fibroblasts are expanded in gelatin pre-coated tissue culture flasks. Pre-coating with a 0.1% gelatin solution is recommended for best attachment.\"}}, {\"@type\": \"Question\", \"name\": \"Is a phenol red-free or serum-free version available?\", \"acceptedAnswer\": {\"@type\": \"Answer\", \"text\": \"Yes. Phenol red-free and serum-free fibroblast medium formats, as well as the basal medium alone, are available on request. Please contact contact@biofargo.com for a quote.\"}}, {\"@type\": \"Question\", \"name\": \"Can it be used for clinical or diagnostic purposes?\", \"acceptedAnswer\": {\"@type\": \"Answer\", \"text\": \"No. This product is for research use only and is not intended for human, diagnostic or therapeutic use.\"}}]}\u003c\/script\u003e\n\u003cdiv class=\"bf-rich\"\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca target=\"_blank\" rel=\"noopener\" href=\"https:\/\/biofargo.com\/pages\/contact\"\u003e\u003cstrong\u003eRequest Datasheet\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"Biofargo","offers":[{"title":"500 mL","offer_id":53408426000565,"sku":"BF-41875525","price":130.0,"currency_code":"USD","in_stock":true}],"url":"https:\/\/biofargo.com\/products\/complete-fibroblast-medium-with-supplement-kit-500-ml","provider":"Biofargo","version":"1.0","type":"link"}