Description

This kit is based on a solid-phase enzyme-linked immunosorbent assay (ELISA) using a double-antibody sandwich technique to quantify CHO PLBL-2 protein in biological samples. A CHO PLBL-2–specific capture antibody is pre-coated onto each well of the microtiter strips. Both calibration standards and test samples are simultaneously added to the wells, followed by incubation and washing. A biotinylated detection antibody is then introduced to bind an epitope of CHO PLBL-2, forming a sandwich complex that subsequently reacts with streptavidin-labeled HRP (SA-HRP). TMB (3,3′,5,5′-tetramethylbenzidine) substrate is added to the reaction and catalyzed by HRP to produce a blue-colored product (maximum absorption at 655 nm). The enzymatic reaction is terminated with the stop solution, yielding a yellow-colored product (maximum absorption at 450 nm). The absorbance at 450 nm is positively correlated with the CHO PLBL-2 concentration in both the calibration standards and the samples. The concentration of CHO PLBL-2 in the samples can be calculated using a dose–response curve.

Overview

Components	Shipping Condition	Unit Size	Detection Method
CHO PLBL-2 Calibration Standard	2-8℃	96 Tests	Sandwich ELISA
Anti-CHO PLBL-2 Microtiter Strips
Reconstitution Solution
Diluent
Wash Buffer Concentrate (10×)
Anti-CHO PLBL-2: Biotinylated Conjugate (200×)
Streptavidin-HRP (100×)
TMB Substrate
Stop Solution
Sealing Film

Specification

Linearity & Range	50-12800 pg/mL, R²>0.990
Accuracy	Recovery Rate= 90.9%-102.4%
LLOQ	50 pg/mL
ULOQ	12800 pg/mL
LOD	13 pg/mL
Precision	13 pg/mL
Specificity	No cross-reactivity with MDCK, Sf9 and E.coli BL21 cells
Robustness	Instrument suitability not limited to Thermo Multiskan FC and MD Spectra Max ABS Microplate Reader
Robustness	Suitable for SHENTEK automatic analytical ELISA system

CHO PLBL-2 HCP ELISA Kit SHENTEK®