Synonym(s): 2-(Cyclohexylamino)ethanesulfonic acid

Description

CHES (2‑Cyclohexylaminoethanesulfonic acid), biochemical grade (≥ 99% purity), is a white crystalline zwitterionic buffer widely used in biochemistry and molecular biology. With high chemical purity, low heavy‑metal content and excellent water solubility, this reagent is designed to prepare reliable buffer solutions without risk of metal‑ion chelation or unwanted side reactions. CHES is especially valuable when stable, non‑coordinating buffering at alkaline pH is required — ensuring reproducible pH conditions for sensitive biological assays.

Specifications

Item	Value
Chemical Name	2-(Cyclohexylamino)Ethanesulfonic Acid
Appearance	White Crystal
CAS No.	103-47-9
Molecular Formula	C8H17NO3S
Molecular Weight	207.3
Grade	Biochemical Grade
Purity	≥99.0%
Water Solubility	Colorless, Clear, Transparent
Heavy Metals	≤5ppm
Moisture	≤0.5%

Features

High purity & quality: ≥ 99.0% purity, biochemical grade; heavy metals ≤ 5 ppm; moisture ≤ 0.5%.
White, water‑soluble crystalline form: dissolves clear and transparent in aqueous solutions, facilitating buffer preparation.
Zwitterionic, non‑coordinating buffer chemistry: minimal metal‑ion binding, making it suitable for metal‑dependent enzymatic assays and reactions.
Optimal for alkaline conditions: effective buffering in the pH range ~ 8.6–10.0 (pKa ≈ 9.3–9.7 at 25 °C), ideal for enzymology and pH‑dependent studies above physiological pH.
Consistent quality control: standardized molecular formula (C₈H₁₇NO₃S), molecular weight (≈ 207.3), and batch‑to‑batch specification to ensure reproducibility.

Applications

Enzymology and pH‑dependent enzyme assays: CHES is widely used as a buffer for studying enzyme activity, kinetics, and stability under mildly alkaline conditions.
Protein purification, folding and stability experiments: Its zwitterionic, non‑chelating nature helps maintain protein integrity and avoid metal‑ion interference in biochemical protocols.
Molecular biology applications: Suitable for buffer preparation in DNA/RNA manipulation, labeling, digestion, or assays that require stable alkaline pH.
Cellular and in‑vitro assays requiring pH control: Useful in cell‑based or in‑vitro experiments where maintenance of an alkaline, stable pH environment is critical.
Buffer formulation for biochemical research and analytical protocols: As a “Good’s buffer,” CHES serves as a reliable standard reagent for buffer preparation in research labs.

Safety Disclaimer

This product is intended for laboratory research use only. Not for drug, human, or veterinary use. Review the Safety Data Sheet (SDS) provided by BioFargo for detailed handling, storage, hazard, and disposal information. Use appropriate personal protective equipment (PPE) including gloves, lab coat, and eye protection when handling. Dispose of waste and residual buffer in accordance with local, state, and federal regulations. Ensure proper ventilation if preparing large volumes or heating solutions.

Documents

SDS

CHES 99% Biochemical Grade