Name: Biofargo cfMSC Pro Canine and Feline MSC Low Serum Complete Medium
Brand: Biofargo
SKU: BFCFMSCPRO500
Availability: InStock

Is Biofargo cfMSC Pro suitable for canine and feline MSC culture?

Yes. Biofargo cfMSC Pro is optimized for canine and feline mesenchymal stem cell culture workflows.

Description

Maintaining stable expansion performance of companion animal mesenchymal stem cells (MSCs) can be challenging during routine veterinary cell culture workflows. Conventional culture systems based on basal media supplemented with fetal bovine serum (FBS) may introduce variability caused by serum batch differences, inconsistent growth support, and difficulty maintaining MSC characteristics across different animal sources.

Biofargo cfMSC Pro Canine and Feline MSC Low Serum Complete Medium is a species-optimized low serum MSC culture medium developed specifically for canine and feline mesenchymal stem cell expansion. The ready-to-use formulation is designed to support consistent MSC proliferation while maintaining the multipotent characteristics required for veterinary stem cell research applications.

Unlike general-purpose mesenchymal stem cell media, Biofargo cfMSC Pro is optimized for companion animal MSC workflows, helping researchers improve culture consistency for different breeds and tissue-derived MSC samples. The complete medium requires no additional growth factor supplementation, providing a simplified workflow for canine MSC culture and feline MSC culture.

Specifications

Product Name	Biofargo cfMSC Pro Canine and Feline MSC Low Serum Complete Medium
SKU	BFCFMSCPRO500-500
Product Type	Veterinary mesenchymal stem cell culture medium
Application Species	Canine and feline MSC culture
Medium Type	Low serum complete medium
Format	Ready-to-use complete medium
Volume	250 mL × 2 bottles
pH	7.2–7.4
Endotoxin	≤0.25 EU/mL
Filtration	<0.2 μm filtration
Bacteria Detection	Not detected
Fungi Detection	Not detected
Storage	4°C
Shelf Life	12 months
Research Grade	For research use only

Features

Species-Optimized MSC Culture Medium — Designed specifically for canine and feline mesenchymal stem cell culture, supporting companion animal MSC workflows across different breeds and tissue sources.
Low Serum Formulation for Improved Consistency — Reduced dependence on traditional FBS-based culture systems to help minimize serum-related variability and improve experimental reproducibility.
Complete Ready-to-Use MSC Expansion Medium — Supplied as a complete medium without additional growth factor supplementation, simplifying canine MSC expansion and feline MSC culture procedures.
Supports MSC Proliferation and Functional Maintenance — Provides a suitable culture environment for maintaining MSC morphology, expansion capability, and multilineage differentiation potential.
Supports Veterinary Stem Cell Research Applications — Suitable for companion animal MSC research including expansion studies, characterization workflows, and differentiation experiments.
Quality-Controlled Manufacturing — Tested for endotoxin level, bacterial contamination, and fungal contamination to support reliable research workflows.

Performance Data

Canine MSC Expansion and Morphology Analysis

Biofargo cfMSC Pro was evaluated using canine mesenchymal stem cells during routine expansion culture. Cells cultured with Biofargo cfMSC Pro maintained typical MSC-like adherent morphology after passaging, demonstrating suitability for canine MSC culture and expansion workflows.

Canine mesenchymal stem cell morphology after expansion using Biofargo cfMSC Pro low serum MSC culture medium

Representative canine MSC morphology after culture with Biofargo cfMSC Pro. The expanded cells display typical spindle-shaped adherent characteristics commonly observed in mesenchymal stem cell cultures.

Feline MSC Expansion and Morphology Analysis

Biofargo cfMSC Pro was also evaluated with feline mesenchymal stem cells. Following passaging, feline MSCs maintained stable attachment and morphology under Biofargo cfMSC Pro culture conditions.

Feline mesenchymal stem cell morphology after expansion using Biofargo cfMSC Pro feline MSC culture medium

Representative feline MSC morphology after expansion with Biofargo cfMSC Pro, supporting its application as a feline mesenchymal stem cell culture medium.

Multilineage Differentiation Capability of Canine MSCs

Maintenance of mesenchymal stem cell differentiation potential is an important consideration during MSC expansion. Biofargo cfMSC Pro-cultured canine MSCs were evaluated for multilineage differentiation capacity, including adipogenic, osteogenic, and chondrogenic differentiation.

Canine MSC trilineage differentiation results after Biofargo cfMSC Pro culture

Canine MSCs expanded using Biofargo cfMSC Pro retained differentiation potential across adipogenic, osteogenic, and chondrogenic lineages under tested induction conditions.

Multilineage Differentiation Capability of Feline MSCs

Feline MSCs expanded with Biofargo cfMSC Pro were evaluated for their ability to undergo adipogenic, osteogenic, and chondrogenic differentiation, demonstrating maintenance of MSC multipotency after culture.

Feline MSC trilineage differentiation analysis after culture with Biofargo cfMSC Pro low serum MSC medium

Representative feline MSC differentiation results demonstrating adipogenic, osteogenic, and chondrogenic differentiation capacity following expansion with Biofargo cfMSC Pro.

Culture Workflow

Biofargo cfMSC Pro is designed as a convenient ready-to-use canine and feline MSC culture medium for routine laboratory expansion workflows.

Slowly thaw the medium at 4°C before use. Avoid repeated freeze-thaw cycles to maintain culture performance.
Use directly after preparation without additional growth factor supplementation.
Recommended seeding density for primary MSC isolation: 5 × 10⁴ cells/cm²
Recommended seeding density for MSC passage: 2–2.5 × 10⁴ cells/cm²
Store unopened medium at -20°C protected from light for long-term storage.
After complete thawing, store at 4°C. Use feline-specific components within 30 days and canine-specific components within 15 days after thawing.

Applications

Biofargo cfMSC Pro is developed for researchers performing veterinary mesenchymal stem cell culture and companion animal stem cell research requiring consistent expansion performance and maintenance of MSC characteristics.

Canine Mesenchymal Stem Cell Culture — Expansion and maintenance of dog-derived MSC populations for veterinary research applications.
Feline Mesenchymal Stem Cell Culture — Supporting cat-derived MSC expansion workflows and characterization studies.
Veterinary MSC Expansion Research — Providing a specialized culture environment for companion animal MSC proliferation studies.
Animal MSC Characterization Studies — Suitable for morphology evaluation, growth studies, and functional analysis.
MSC Trilineage Differentiation Research — Supports adipogenic, osteogenic, and chondrogenic differentiation experiments.
Companion Animal Regenerative Medicine Research — Applicable to laboratory research related to veterinary regenerative medicine and stem cell biology.
Animal Cell Banking Research — Supports expansion workflows for establishing research-scale canine and feline MSC collections.
Comparative Stem Cell Biology Studies — Suitable for investigations involving species-specific MSC characteristics.

Why Choose Biofargo cfMSC Pro?

Developing reliable veterinary mesenchymal stem cell culture workflows requires a balance between efficient cell expansion, maintenance of MSC characteristics, and consistent experimental performance. Conventional animal cell culture approaches using general basal media with serum supplementation may result in variable outcomes due to serum batch differences and species-specific culture requirements.

Biofargo cfMSC Pro is designed as a dedicated canine and feline MSC culture medium, providing a species-optimized low serum environment for companion animal stem cell research. By focusing on the specific requirements of canine and feline mesenchymal stem cells, Biofargo cfMSC Pro helps researchers establish more consistent MSC expansion workflows while maintaining differentiation capability.

Compared with general-purpose MSC media, Biofargo cfMSC Pro provides a specialized solution for veterinary MSC researchers who require a reliable culture system for dog MSC expansion, cat MSC culture, and companion animal regenerative medicine research applications.

FAQ

What is Biofargo cfMSC Pro Canine and Feline MSC Culture Medium used for?

Biofargo cfMSC Pro is a low serum complete culture medium designed for canine and feline mesenchymal stem cell expansion, maintenance, and differentiation research. It is intended for research use involving companion animal MSC culture workflows.

Is Biofargo cfMSC Pro suitable for canine MSC culture?

Yes. Biofargo cfMSC Pro is optimized for canine mesenchymal stem cell culture and supports canine MSC expansion, morphology maintenance, and multilineage differentiation research.

Is Biofargo cfMSC Pro suitable for feline MSC culture?

Yes. Biofargo cfMSC Pro is designed for feline mesenchymal stem cell culture, providing a specialized medium environment for cat MSC expansion and characterization studies.

What is the advantage of using a species-optimized MSC medium instead of general MSC culture media?

Species-optimized MSC media are designed according to the biological characteristics of specific animal sources. Biofargo cfMSC Pro is developed specifically for canine and feline MSC workflows to improve culture consistency compared with non-specialized MSC culture systems.

Is Biofargo cfMSC Pro a serum-free MSC medium?

Biofargo cfMSC Pro is a low serum MSC culture medium. It is designed to reduce dependence on traditional serum-based culture systems while supporting canine and feline MSC expansion and maintenance.

Does Biofargo cfMSC Pro require additional growth factors before use?

No. Biofargo cfMSC Pro is supplied as a ready-to-use complete medium and does not require additional growth factor supplementation before culture.

What cells can be cultured with Biofargo cfMSC Pro?

Biofargo cfMSC Pro is intended for canine and feline mesenchymal stem cell culture, including MSCs derived from different tissues and breeds used in veterinary stem cell research.

Does Biofargo cfMSC Pro support MSC differentiation research?

Yes. Biofargo cfMSC Pro-cultured canine and feline MSCs have been evaluated for multilineage differentiation capability, including adipogenic, osteogenic, and chondrogenic differentiation.

What differentiation capabilities were evaluated with Biofargo cfMSC Pro?

The product evaluation included three commonly studied MSC differentiation directions: adipogenic differentiation, osteogenic differentiation, and chondrogenic differentiation.

What is the recommended seeding density for canine and feline MSC culture?

For primary MSC isolation workflows, a recommended seeding density of 5 × 10⁴ cells/cm² is provided. For passaged MSC culture, 2–2.5 × 10⁴ cells/cm² is recommended.

How should Biofargo cfMSC Pro be stored?

For long-term storage, Biofargo cfMSC Pro should be stored at -20°C protected from light. After thawing, maintain the medium at 4°C. Cat-specific components should be used within 30 days after thawing, while dog-specific components should be used within 15 days.

What quality control tests are performed for Biofargo cfMSC Pro?

Biofargo cfMSC Pro is tested for endotoxin level, bacterial contamination, and fungal contamination. The product has an endotoxin specification of ≤0.25 EU/mL and is filtered through a <0.2 μm filtration process.

cfMSC Pro – Canine/Feline MSC Complete Medium