Description

This kit uses the spin column and formula developed by Foregene, which can efficiently extract high-purity and high-quality total RNA from cells cultured in 96, 24, 12, and 6-well plates. The kit provides an efficient DNA-Cleaning Column, which can easily separate the supernatant and cell lysate, bind and remove genomic DNA. The operation is simple and time-saving; the RNA-only Column can efficiently bind RNA with a unique formula. A large number of samples can be processed simultaneously.

The whole system is RNase-Free, so that the purified RNA is not degraded; The Buffer RW1 and Buffer RW2 washing system guarantees that the obtained RNA is free of protein, DNA, ions, and organic compound contamination.

Features

The whole process is operated at room temperature (15-25℃), without ice bath and low temperature centrifugation.
The whole kit is RNase-Free, no need to worry about RNA degradation.
DNA-Cleaning Column specifically binds DNA, so that the kit can remove genomic DNA contamination without adding additional DNase.
High RNA yield: RNA-only Column and unique formula can efficiently purify RNA.
Fast speed: easy to operate and can be completed in 11 minutes.
Safety: No organic reagent is required.
High quality: The purified RNA is of high purity, free of protein and other impurities, and can meet various subsequent experiments.

Application

It is suitable for extraction and purification of total RNA from cultured cells in 96, 24, 12, and 6-well plates.

Work Flow

Diagram

The agarose gel electrophoresis image of total RNA isolated using the Cell Total RNA Isolation Kit from different cell numbers, with 20 μl elution volume and 2 μl purified total RNA loaded on a 1% gel.

Storage

The kit can be stored for 12 months at room temperature (15–25 ℃) or 2–8 ℃ for longer time(24 months).

Buffer CRL1 can be stored at 4 ℃ for 1 month after adding 2-hydroxy-1-ethanethiol (optional).

Documents

Cell Total RNA Isolation Kit Instruction Manual-V1.0-EN