Cell Counting Kit (CCK-8) CCK-8

Cell Counting Kit-8 (CCK-8) is a fast and highly sensitive detection kit based on the WST-8 (2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium monosodium salt) reagent. WST-8 is an upgraded reagent of MTT and can be reduced to a highly water-soluble orange-yellow formazan by dehydrogenases in the mitochondria. The amount of the formazan generated is proportional to the number of living cells. The OD value of formazan at 450nm detected by a microplate reader can indirectly indicate the number of viable cells. This kit is widely used for drug screening, cell proliferation and cytotoxicity assays, tumor drug sensitivity testing, and activity detection of biological factors.