Description

The Cell Counting Kit-8 (CCK-8) uses a water-soluble tetrazole salt WST-8 that is bioreduced in live cells to produce a methane dye. This enzymatic reduction relies on intracellular dehydrogenase activity, allowing for a direct correlation between the amount of methane produced and the number of viable cells. The water solubility of the resulting methane simplifies the assay procedure and can be quantified by a microplate reader. This kit is commonly used in biomedical research to assess cell proliferation, viability, and cytotoxicity in vitro. Compared to methods like MTT, XTT, MTS, or WST-1, CCK-8 offers increased sensitivity and ease of use.

Specifications

Cell Counting Kit-8(CCK-8)

Catalog Number	C6005S	C6005M	C6005L	C6005XL	C6005XXL
Product Size	100T	500T	3000T	10000T	5×10000T
Component	1 mL	5 mL	30 mL	100 mL	5×100 mL

Features

More Sensitive than MTT, MTS, or WST-1
No Toxicity to Cell
Simpler Steps, No organic solvents required
Stable One Bottle Solution, ready-to-use

Advantages

Figure

1. Principle of the CCK-8 detection

Figure 2. Cell number determination using CCK-8 reagent

Storage

Storage Store at 4℃ and protect from light. Long-term storage can be placed at -20℃. Expiration date marked on the outer packing.

Documents

C6005

C6005 MSDS

Product Citation

1. Min Liu, Xiaoling Ren, et al. "Corin: a dual inhibitor for KDM1A/HDAC1, suppresses hepatocellular carcinoma by triggering cuproptosis." Clin Exp Med 26, 33 (2026).

2. Honglei Cheng, Yueju Ding, et al. "TMEM165 promotes glioblastoma progression through epithelial-mesenchymal transition-mediated invasion and temozolomide chemoresistance." International Journal of Biological Macromolecules Volume 335, Part 1, January 2026, 149216

3. Yuanyuan Qin, Hongli Zhou, et al. "AIF1L as a Ferroptosis-Linked Biomarker in Microsatellite States–Driven Colorectal Cancer: Functional and Diagnostic Insights From Multiomics Analysis." Hum Mutat. 2025 Oct 10:2025:6663166 PMID: 41113993

4. Xi Chen, MengXue Zhang, et al. "Diosgenin activates ERa-dependent PI3K/AKT/GSK3b signaling to enhance brain-derived serotonin and alleviate postmenopausal bone loss." J Adv Res. 2025 Nov 24:S2090-1232(25)00949-X PMID: 41297791

5. Rui Wang, Bin Wang, et al. "Apolipoprotein C1 functions as a target of thyroid carcinoma and synergistic effects with promising candidate-cyclopamine." Translational Oncology Volume 63, January 2026, 102617 PMID: 41289702

Cell Proliferation Detection Reagent-C6005S