{"product_id":"catarosev-small-ev-and-exosome-isolation-kit-toyobo","title":"CATAROSEV Small EV and Exosome Isolation Kit-Toyobo","description":"\u003carticle\u003e\n  \u003ch2\u003eDescription\u003c\/h2\u003e\n\n  \u003cp\u003eRecovering small extracellular vesicles from cell culture supernatant, serum, or plasma can involve complicated sample preparation, sample concentration, or expensive equipment. CATAROSEV® Small EV Isolation Kit provides a membrane-based solution for separating and recovering small EVs without complicated sample concentration or ultracentrifugation equipment.\u003c\/p\u003e\n\n  \u003cp\u003eCATAROSEV® uses a precision filtration membrane with ion-exchange functionality and size-separation capability. The small EV isolation workflow uses the size and surface charge of extracellular vesicles to complete adsorption, washing, and elution. Small EVs adsorbed to the membrane are recovered with a high-salt elution buffer.\u003c\/p\u003e\n\n  \u003cp\u003eThe procedure can be completed in approximately 30 minutes. According to the stated product information, the kit efficiently recovers intact particles within the 50–150 nm range. The product has been used to recover small EVs from samples including cell culture supernatant, serum, plasma, fruit juice, bacterial samples, tomato fruit, and peripheral blood.\u003c\/p\u003e\n\n  \u003cp\u003eCatalog number CTS-001 contains five single-use syringe filters and five three-way stopcocks. The required salt concentration adjustment, equilibration\/washing, and elution buffers are not included.\u003c\/p\u003e\n\n  \u003ch2\u003eSpecification\u003c\/h2\u003e\n\n  \u003cdiv style=\"width:100%; overflow-x:auto; -webkit-overflow-scrolling:touch;\"\u003e\n    \u003ctable style=\"width:100%; min-width:640px; border-collapse:collapse;\"\u003e\n      \u003ctbody\u003e\n        \u003ctr\u003e\n          \u003cth scope=\"row\" style=\"padding:10px; border:1px solid #dddddd; text-align:left;\"\u003eProduct Name\u003c\/th\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #dddddd;\"\u003eCATAROSEV® Small EV Isolation Kit\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003cth scope=\"row\" style=\"padding:10px; border:1px solid #dddddd; text-align:left;\"\u003eCatalog Number\u003c\/th\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #dddddd;\"\u003eCTS-001\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003cth scope=\"row\" style=\"padding:10px; border:1px solid #dddddd; text-align:left;\"\u003eProduct Type\u003c\/th\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #dddddd;\"\u003eSmall EV and Exosome Isolation Kit\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003cth scope=\"row\" style=\"padding:10px; border:1px solid #dddddd; text-align:left;\"\u003ePurpose\u003c\/th\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #dddddd;\"\u003eIsolation and recovery of small extracellular vesicles\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003cth scope=\"row\" style=\"padding:10px; border:1px solid #dddddd; text-align:left;\"\u003eIsolation Principle\u003c\/th\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #dddddd;\"\u003eIon exchange and size separation\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003cth scope=\"row\" style=\"padding:10px; border:1px solid #dddddd; text-align:left;\"\u003eMembrane Pore Size\u003c\/th\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #dddddd;\"\u003eApproximately 200 nm\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003cth scope=\"row\" style=\"padding:10px; border:1px solid #dddddd; text-align:left;\"\u003eTarget Particle Range\u003c\/th\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #dddddd;\"\u003e50–150 nm\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003cth scope=\"row\" style=\"padding:10px; border:1px solid #dddddd; text-align:left;\"\u003eWorkflow\u003c\/th\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #dddddd;\"\u003eAdsorption, washing, and elution\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003cth scope=\"row\" style=\"padding:10px; border:1px solid #dddddd; text-align:left;\"\u003eProcessing Time\u003c\/th\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #dddddd;\"\u003eApproximately 30 minutes\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003cth scope=\"row\" style=\"padding:10px; border:1px solid #dddddd; text-align:left;\"\u003eRecommended Cell Culture Supernatant Input\u003c\/th\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #dddddd;\"\u003eApproximately 10–15 mL\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003cth scope=\"row\" style=\"padding:10px; border:1px solid #dddddd; text-align:left;\"\u003eRecommended Serum Input\u003c\/th\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #dddddd;\"\u003eApproximately 100–200 μL\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003cth scope=\"row\" style=\"padding:10px; border:1px solid #dddddd; text-align:left;\"\u003eRecommended Plasma Input\u003c\/th\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #dddddd;\"\u003eApproximately 100–200 μL\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003cth scope=\"row\" style=\"padding:10px; border:1px solid #dddddd; text-align:left;\"\u003eElution Buffer Volume\u003c\/th\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #dddddd;\"\u003e2 mL\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003cth scope=\"row\" style=\"padding:10px; border:1px solid #dddddd; text-align:left;\"\u003eApproximate Eluate Salt Concentration\u003c\/th\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #dddddd;\"\u003e0.25 M\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003cth scope=\"row\" style=\"padding:10px; border:1px solid #dddddd; text-align:left;\"\u003ePack Size\u003c\/th\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #dddddd;\"\u003e5 uses\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003cth scope=\"row\" style=\"padding:10px; border:1px solid #dddddd; text-align:left;\"\u003eStorage\u003c\/th\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #dddddd;\"\u003eRefrigerated storage\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003cth scope=\"row\" style=\"padding:10px; border:1px solid #dddddd; text-align:left;\"\u003eTemporary Room-Temperature Storage\u003c\/th\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #dddddd;\"\u003eUp to 3 months\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003cth scope=\"row\" style=\"padding:10px; border:1px solid #dddddd; text-align:left;\"\u003eReuse\u003c\/th\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #dddddd;\"\u003eSingle use only\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003cth scope=\"row\" style=\"padding:10px; border:1px solid #dddddd; text-align:left;\"\u003eIntended Use\u003c\/th\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #dddddd;\"\u003eResearch use only\u003c\/td\u003e\n        \u003c\/tr\u003e\n      \u003c\/tbody\u003e\n    \u003c\/table\u003e\n  \u003c\/div\u003e\n\n  \u003ch3\u003eKit Components\u003c\/h3\u003e\n\n  \u003cdiv style=\"width:100%; overflow-x:auto; -webkit-overflow-scrolling:touch;\"\u003e\n    \u003ctable style=\"width:100%; min-width:520px; border-collapse:collapse;\"\u003e\n      \u003cthead\u003e\n        \u003ctr\u003e\n          \u003cth scope=\"col\" style=\"padding:10px; border:1px solid #dddddd; text-align:left;\"\u003eComponent\u003c\/th\u003e\n          \u003cth scope=\"col\" style=\"padding:10px; border:1px solid #dddddd; text-align:left;\"\u003eQuantity\u003c\/th\u003e\n        \u003c\/tr\u003e\n      \u003c\/thead\u003e\n      \u003ctbody\u003e\n        \u003ctr\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #dddddd;\"\u003eSyringe filter with ion-exchange membrane\u003c\/td\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #dddddd;\"\u003e5 sets\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #dddddd;\"\u003eThree-way stopcock\u003c\/td\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #dddddd;\"\u003e5 sets\u003c\/td\u003e\n        \u003c\/tr\u003e\n      \u003c\/tbody\u003e\n    \u003c\/table\u003e\n  \u003c\/div\u003e\n\n  \u003ch3\u003eRequired but Not Included\u003c\/h3\u003e\n\n  \u003cul\u003e\n    \u003cli\u003eSample containing extracellular vesicles\u003c\/li\u003e\n    \u003cli\u003eFour 20 mL syringes per procedure\u003c\/li\u003e\n    \u003cli\u003eOne 5 mL collection tube per procedure\u003c\/li\u003e\n    \u003cli\u003eBenchtop centrifuge and centrifuge tubes\u003c\/li\u003e\n    \u003cli\u003eProtective equipment, including protective glasses and gloves\u003c\/li\u003e\n    \u003cli\u003eSalt concentration adjustment buffer\u003c\/li\u003e\n    \u003cli\u003eEquilibration and washing buffer\u003c\/li\u003e\n    \u003cli\u003eElution buffer\u003c\/li\u003e\n  \u003c\/ul\u003e\n\n  \u003cp\u003eA low-protein-binding collection tube is recommended. The three required buffers can be prepared according to the instructions or obtained separately as Buffer Set for CATAROSEV®, catalog number CTS-011.\u003c\/p\u003e\n\n  \u003ch2\u003eFeatures\u003c\/h2\u003e\n\n  \u003cul\u003e\n    \u003cli\u003eMembrane-based small EV isolation using particle size and surface charge.\u003c\/li\u003e\n    \u003cli\u003ePrecision filtration membrane with ion-exchange and size-separation functions.\u003c\/li\u003e\n    \u003cli\u003eApproximately 30-minute small EV recovery procedure.\u003c\/li\u003e\n    \u003cli\u003eNo complicated sample concentration is required.\u003c\/li\u003e\n    \u003cli\u003eNo expensive ultracentrifugation equipment is required.\u003c\/li\u003e\n    \u003cli\u003eThree-step adsorption, washing, and elution workflow.\u003c\/li\u003e\n    \u003cli\u003eEfficient recovery of intact particles within the 50–150 nm range.\u003c\/li\u003e\n    \u003cli\u003eApplicable to cell culture supernatant, serum, and plasma sample preparation.\u003c\/li\u003e\n    \u003cli\u003eFive single-use isolation sets per CTS-001 kit.\u003c\/li\u003e\n  \u003c\/ul\u003e\n\n  \u003ch3\u003eIsolation Principle\u003c\/h3\u003e\n\n  \u003col\u003e\n    \u003cli\u003e\n\u003cstrong\u003eAdsorption:\u003c\/strong\u003e The prepared sample is introduced into the CATAROSEV® membrane, where small EVs adsorb through their surface charge.\u003c\/li\u003e\n    \u003cli\u003e\n\u003cstrong\u003eWashing:\u003c\/strong\u003e Equilibration and washing buffer is introduced to remove uncharged contaminants.\u003c\/li\u003e\n    \u003cli\u003e\n\u003cstrong\u003eElution:\u003c\/strong\u003e A high-salt elution buffer is used to recover the small EVs adsorbed within the membrane.\u003c\/li\u003e\n  \u003c\/ol\u003e\n\n  \u003ch2\u003eApplication\u003c\/h2\u003e\n\n  \u003cp\u003eCATAROSEV® is intended for the isolation and recovery of small extracellular vesicles from research samples. The stated sample formats include cell culture supernatant, serum, and plasma.\u003c\/p\u003e\n\n  \u003cul\u003e\n    \u003cli\u003eSmall EV isolation from cell culture supernatant\u003c\/li\u003e\n    \u003cli\u003eSmall EV isolation from serum\u003c\/li\u003e\n    \u003cli\u003eSmall EV isolation from plasma\u003c\/li\u003e\n    \u003cli\u003eExtracellular vesicle particle-size analysis\u003c\/li\u003e\n    \u003cli\u003eWestern blot analysis of recovered extracellular vesicles\u003c\/li\u003e\n    \u003cli\u003eMALDI-TOF MS analysis of extracellular vesicles recovered from peripheral blood\u003c\/li\u003e\n  \u003c\/ul\u003e\n\n  \u003cp\u003eAdditional examples presented in the product information include extracellular vesicle recovery from bacterial samples and tomato fruit. Sample suitability may vary according to sample composition and preparation conditions.\u003c\/p\u003e\n\n  \u003ch2\u003eFAQ\u003c\/h2\u003e\n\n  \u003cdetails\u003e\n    \u003csummary\u003e\u003cstrong\u003eWhat is the CATAROSEV® Small EV Isolation Kit used for?\u003c\/strong\u003e\u003c\/summary\u003e\n    \u003cp\u003eCATAROSEV® is used to isolate and recover small extracellular vesicles from research samples using a precision filtration membrane with ion-exchange and size-separation functions.\u003c\/p\u003e\n  \u003c\/details\u003e\n\n  \u003cdetails\u003e\n    \u003csummary\u003e\u003cstrong\u003eHow does this membrane-based exosome isolation kit work?\u003c\/strong\u003e\u003c\/summary\u003e\n    \u003cp\u003eThe kit separates small EVs according to particle size and surface charge. The workflow consists of adsorption to the membrane, washing to remove contaminants, and high-salt elution to recover the adsorbed small EVs.\u003c\/p\u003e\n  \u003c\/details\u003e\n\n  \u003cdetails\u003e\n    \u003csummary\u003e\u003cstrong\u003eDoes CATAROSEV® require ultracentrifugation?\u003c\/strong\u003e\u003c\/summary\u003e\n    \u003cp\u003eNo expensive ultracentrifugation equipment is required for the stated CATAROSEV® isolation workflow.\u003c\/p\u003e\n  \u003c\/details\u003e\n\n  \u003cdetails\u003e\n    \u003csummary\u003e\u003cstrong\u003eIs sample concentration required before small EV isolation?\u003c\/strong\u003e\u003c\/summary\u003e\n    \u003cp\u003eThe product does not require complicated pretreatment such as sample concentration. Sample-specific preparation is still required according to the instructions.\u003c\/p\u003e\n  \u003c\/details\u003e\n\n  \u003cdetails\u003e\n    \u003csummary\u003e\u003cstrong\u003eHow long does the small EV recovery procedure take?\u003c\/strong\u003e\u003c\/summary\u003e\n    \u003cp\u003eThe stated processing time is approximately 30 minutes.\u003c\/p\u003e\n  \u003c\/details\u003e\n\n  \u003cdetails\u003e\n    \u003csummary\u003e\u003cstrong\u003eWhat particle size range can be recovered?\u003c\/strong\u003e\u003c\/summary\u003e\n    \u003cp\u003eCATAROSEV® is designed to efficiently recover intact particles within the 50–150 nm range.\u003c\/p\u003e\n  \u003c\/details\u003e\n\n  \u003cdetails\u003e\n    \u003csummary\u003e\u003cstrong\u003eWhat is the approximate membrane pore size?\u003c\/strong\u003e\u003c\/summary\u003e\n    \u003cp\u003eThe precision filtration membrane has an approximate pore size of 200 nm.\u003c\/p\u003e\n  \u003c\/details\u003e\n\n  \u003cdetails\u003e\n    \u003csummary\u003e\u003cstrong\u003eCan the kit isolate small EVs from cell culture supernatant?\u003c\/strong\u003e\u003c\/summary\u003e\n    \u003cp\u003eYes. The recommended cell culture supernatant input is approximately 10–15 mL.\u003c\/p\u003e\n  \u003c\/details\u003e\n\n  \u003cdetails\u003e\n    \u003csummary\u003e\u003cstrong\u003eWhat preparation is recommended for cell culture supernatant?\u003c\/strong\u003e\u003c\/summary\u003e\n    \u003cp\u003eIf coarse particles may be present, prefiltration with a 0.45 μm filter is recommended. The sample should be adjusted to a salt concentration of approximately 0.1 M using salt concentration adjustment buffer.\u003c\/p\u003e\n  \u003c\/details\u003e\n\n  \u003cdetails\u003e\n    \u003csummary\u003e\u003cstrong\u003eCan CATAROSEV® be used with serum or plasma?\u003c\/strong\u003e\u003c\/summary\u003e\n    \u003cp\u003eYes. The recommended serum or plasma input is approximately 100–200 μL.\u003c\/p\u003e\n  \u003c\/details\u003e\n\n  \u003cdetails\u003e\n    \u003csummary\u003e\u003cstrong\u003eHow should serum or plasma be prepared?\u003c\/strong\u003e\u003c\/summary\u003e\n    \u003cp\u003eSerum or plasma should be centrifuged at 10,000 × g for 5 minutes at room temperature. The recovered supernatant should then be diluted at least 50-fold with equilibration and washing buffer.\u003c\/p\u003e\n  \u003c\/details\u003e\n\n  \u003cdetails\u003e\n    \u003csummary\u003e\u003cstrong\u003eWhat components are included with catalog number CTS-001?\u003c\/strong\u003e\u003c\/summary\u003e\n    \u003cp\u003eCTS-001 contains five syringe filters with ion-exchange membranes and five three-way stopcocks.\u003c\/p\u003e\n  \u003c\/details\u003e\n\n  \u003cdetails\u003e\n    \u003csummary\u003e\u003cstrong\u003eAre the required buffers included in CTS-001?\u003c\/strong\u003e\u003c\/summary\u003e\n    \u003cp\u003eNo. Salt concentration adjustment buffer, equilibration and washing buffer, and elution buffer are not included.\u003c\/p\u003e\n  \u003c\/details\u003e\n\n  \u003cdetails\u003e\n    \u003csummary\u003e\u003cstrong\u003eIs a separate buffer set available?\u003c\/strong\u003e\u003c\/summary\u003e\n    \u003cp\u003eYes. Buffer Set for CATAROSEV®, catalog number CTS-011, contains 100 mL each of salt concentration adjustment buffer, equilibration and washing buffer, and elution buffer.\u003c\/p\u003e\n  \u003c\/details\u003e\n\n  \u003cdetails\u003e\n    \u003csummary\u003e\u003cstrong\u003eHow much washing buffer is used per procedure?\u003c\/strong\u003e\u003c\/summary\u003e\n    \u003cp\u003eThe membrane equilibration step uses 5 mL of equilibration and washing buffer. The washing step uses an additional 10 mL.\u003c\/p\u003e\n  \u003c\/details\u003e\n\n  \u003cdetails\u003e\n    \u003csummary\u003e\u003cstrong\u003eHow much elution buffer is used?\u003c\/strong\u003e\u003c\/summary\u003e\n    \u003cp\u003eThe elution step uses 2 mL of elution buffer.\u003c\/p\u003e\n  \u003c\/details\u003e\n\n  \u003cdetails\u003e\n    \u003csummary\u003e\u003cstrong\u003eWhat is the approximate salt concentration of the recovered eluate?\u003c\/strong\u003e\u003c\/summary\u003e\n    \u003cp\u003eThe recovered eluate has an approximate salt concentration of 0.25 M. The salt concentration can be adjusted when required for subsequent experiments.\u003c\/p\u003e\n  \u003c\/details\u003e\n\n  \u003cdetails\u003e\n    \u003csummary\u003e\u003cstrong\u003eCan a CATAROSEV® syringe filter be reused?\u003c\/strong\u003e\u003c\/summary\u003e\n    \u003cp\u003eNo. Each filter is intended for a single use and should not be washed or reused.\u003c\/p\u003e\n  \u003c\/details\u003e\n\n  \u003cdetails\u003e\n    \u003csummary\u003e\u003cstrong\u003eHow should CATAROSEV® be stored?\u003c\/strong\u003e\u003c\/summary\u003e\n    \u003cp\u003eThe kit should be stored under refrigerated conditions. Storage at room temperature is possible for up to three months.\u003c\/p\u003e\n  \u003c\/details\u003e\n\n  \u003cdetails\u003e\n    \u003csummary\u003e\u003cstrong\u003eWhat precautions should be taken during operation?\u003c\/strong\u003e\u003c\/summary\u003e\n    \u003cp\u003eAir should not be allowed to enter the syringe filter because trapped air may prevent an appropriate recovery volume. Samples should also be handled using protective equipment and according to the applicable laboratory safety requirements.\u003c\/p\u003e\n  \u003c\/details\u003e\n\n  \u003cdetails\u003e\n    \u003csummary\u003e\u003cstrong\u003eIs CATAROSEV® intended for diagnostic or therapeutic use?\u003c\/strong\u003e\u003c\/summary\u003e\n    \u003cp\u003eNo. CATAROSEV® is a research-use reagent. It must not be used as food or as a cosmetic product.\u003c\/p\u003e\n  \u003c\/details\u003e\n\u003c\/article\u003e","brand":"Toyobo","offers":[{"title":"1 Use","offer_id":53106932187317,"sku":"CTS-001T","price":0.0,"currency_code":"USD","in_stock":true},{"title":"5 Uses","offer_id":53106932220085,"sku":"CTS-001","price":0.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/catarosev-cts-001-product-1500x1500.jpg?v=1787906115","url":"https:\/\/biofargo.com\/products\/catarosev-small-ev-and-exosome-isolation-kit-toyobo","provider":"Biofargo","version":"1.0","type":"link"}