Description

Cas13a is an RNA-guided RNA endonuclease that belongs to the class 2 type VI CRISPR-Cas systems (1). Cas13a possesses both RNA recognition and cleavage activities, cleaving RNA through conserved basic residues within its two HEPN domains (2). Activated Cas13a also induces collateral cleavage of nearby non-targeted RNAs in a nonspecific manner (3). This property of the Cas13a enzyme has been leveraged for the development of sensitive and robust nucleic acid detection tools with applications in both basic research and therapeutics development (4).

Components

	Part Number	Part	Size
Ice Pack	CS13A-E322U	LbuCas13a, Active	100 µg/1
	CS13-09B	Cas13 Reaction Buffer II, 10×	1 ml/2
	CS13-57	Cas13 ssRNA Reporter	10 nmol/1
	CS13-G58	Cas13 Guide RNA (custom)	1 ea/1

Documents

Cas13 (LbuCas13a) Kit, Buffer, Reporter, gRNA

References

East-Seletsky A, et al. 2016. Two distinct RNase activities of CRISPR-C2c2 enable guide-RNA processing and RNA detection. Nature. 538:270–273
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Abudayyeh O, et al. 2016. C2c2 is a single-component programmable RNA-guided RNA-targeting CRISPR effector. Science. 353:5573.
Zhang J, You Y. 2020. CRISPR-Cas13a system: A novel approach to precision oncology. Cancer Biol Med. 17(1):6-8.
Patchsung M, et al. 2020. Clinical validation of a Cas13-based assay for the detection of SARS-CoV-2 RNA. Nat Biomed Eng. 4(12):1140-1149.