Description

CRISPR (clustered regularly interspaced short palindromic repeats) and CRISPR-associated (Cas) proteins constitute the adaptive immune system in bacteria (1-2). This system has been leveraged to create an exemplary gene detection and genome editing tool with applications in both basic research and therapeutics development (3). AapCas12b belongs to the type V CRISPR effector, CRISPRCas12b/C2c1. It has both cis and trans nuclease cleavage activity. Due to its trans cleavage activity and excellent thermostability, it can be used in a wide range of biochemical applications including gene detection, mammalian genome editing, and gene activation (4).

Components

	Part Number	Part	Size
Ice Pack	C12B1-E311U	AapCas12b, Active	100 µg/1
	CS12-09	Cas12 Reaction Buffer, 10×	1 ml/2
	CS12-57	Cas12 ssDNA Reporter	10 nmol/1
	CS12-G58	Cas12 Guide RNA (custom)	1 ea/1

Documents

Cas12 (AapCas12b) Kit, Buffer, Reporter, gRNA

References

Horvath, P., et al. 2010. CRISPR/Cas, the immune system of bacteria and archaea. Science, 327(5962):167-170.
Morange, M. 2015. What history tells us XXXVII. CRISPR-Cas: The discovery of an immune system in prokaryotes. J. Biosci. 40(2):221-223.
Gier, R.A., et al. 2020. High-performance CRISPR-Cas12a genome editing for combinatorial genetic screening. Nat. Commun. 11(1):1-9.
Teng F, et al. 2018. Repurposing CRISPR-Cas12b for mammalian genome engineering. Cell Discovery. 2018, 4(1):1-5.