Name: Carboxypeptidase A from Bovine Pancreas
SKU: BF-2060050

Question 1

What is Carboxypeptidase A?

Accepted Answer

Carboxypeptidase A (EC 3.4.17.1), also known as peptidyl-L-amino acid hydrolase, is a zinc-containing pancreatic exopeptidase from bovine pancreas with a molecular weight of approximately 35,000 Da. It preferentially hydrolyzes peptide bonds at C-terminal aromatic amino acids.

Question 2

What is the source of this Carboxypeptidase A?

Accepted Answer

This Carboxypeptidase A is derived from bovine pancreas.

Question 3

Which amino acids does Carboxypeptidase A cleave?

Accepted Answer

Carboxypeptidase A preferentially hydrolyzes C-terminal aromatic amino acids — phenylalanine, tyrosine and tryptophan. Glycine, aspartic acid and glutamic acid are hydrolyzed slowly, while arginine, proline and hydroxyproline are not hydrolyzed.

Question 4

What is the optimal pH of Carboxypeptidase A?

Accepted Answer

The optimal pH range of Carboxypeptidase A is 7-8.

Question 5

How is Carboxypeptidase A activity defined?

Accepted Answer

One unit of Carboxypeptidase A hydrolyzes 1 μmol of hippuryl-L-phenylalanine per minute at 25°C and pH 7.5.

Question 6

How is Carboxypeptidase A activity measured?

Accepted Answer

Activity is measured by the increase in absorbance at 254 nm during hydrolysis of hippuryl-L-phenylalanine. The initial linear rate (ΔE254/min) is used in the formula: Activity (U/mg) = (ΔE254/min × Total Vol. × Enz. Diln.) / (0.36 × mg Enz./ml).

Question 7

Is Carboxypeptidase A a metalloenzyme?

Accepted Answer

Yes. Carboxypeptidase A is a zinc-containing metalloenzyme; the zinc ion is tightly coordinated by two histidine imidazole rings and the carboxyl group of one glutamic acid residue.

Question 8

How should the enzyme be prepared for the activity assay?

Accepted Answer

Dissolve Carboxypeptidase A in 10% LiCl to 1-3 U/ml, and add to a 1 mM hippuryl-L-phenylalanine solution in 50 mM Tris/HCl buffer (containing 1.0 M NaCl), pH 7.5. Monitor the increase in absorbance at 254 nm over 5 minutes at 25°C.

Product Name	Carboxypeptidase A
Synonyms	Peptidyl-L-amino acid hydrolase
Catalog Number	BF-2060050
EC Number	3.4.17.1
Biological Source	Bovine pancreas
Molecular Weight	Approximately 35,000 Da
Optimal pH	7-8
Unit Definition	One unit hydrolyzes 1 µmol hippuryl-L-phenylalanine per minute at 25°C, pH 7.5
Assay Method	Increase in absorbance at 254 nm during hydrolysis of hippuryl-L-phenylalanine

Description

Specification

Features

Application

FAQ