Synonym(s): 3-(Cyclohexylamino)-2-hydroxy-1-propanesulfonic acid, CAPSO Free Acid

Description

CAPSO (3‑(Cyclohexylamino)-2-hydroxy-1-propanesulfonic acid) is a high‑purity (≥ 99.0%) biochemical grade buffer reagent designed for demanding molecular biology, biochemistry, and protein science applications. As a zwitterionic buffer, CAPSO offers excellent water solubility and minimal metal ion chelation — features that make it especially suitable for experiments where metal‑dependent enzymes or sensitive proteins are involved. With a pKa around 9.6 (25 °C), CAPSO is optimized for alkaline conditions, providing robust pH stability in its effective buffering range.

Because of its chemical stability, low UV absorbance, and negligible interaction with proteins or metal ions, CAPSO is widely used in laboratory protocols requiring non‑interfering buffers — such as protein transfer, electrophoresis, enzyme assays, and other conditions where maintaining precise pH is critical.

Specifications

Item	Value
Chemical Name	3-(Cyclohexylamino)-2-Hydroxy-1-Propanesulfinic Acid
Appearance	White Crystalline Powder
CAS No.	73463-39-5
Molecular Formula	C9H19NO4S
Molecular Weight	237.3
Grade	Biochemical Grade
Purity	≥99.0%
Water Solubility	Colorless, Clear
Heavy Metals	≤3PPM
Moisture	≤1.0%
pH	5.0-6.5
UV Absorbance at 260nm	≤0.040
UV Absorbance at 280nm	≤0.030
Heavy Metals	≤3PPM
Iron Content	≤5PPM
Storage / Handling	Store at Room Temperature.

Features

High Purity & Quality: ≥ 99.0% biochemical grade with low heavy metals (≤ 3 ppm) and minimal moisture (≤ 1.0%), ensuring consistency in sensitive experiments.
Zwitterionic Buffer with Low Metal Binding: Minimal chelation to metal ions, critical for studies involving metal-dependent enzymes or proteins.
Alkaline Buffer Stability: With a pKa ~9.6 at 25 °C, CAPSO buffers effectively in the pH range of 8.9–10.3.
Excellent Solubility: Dissolves readily in water to form clear, colorless solutions, facilitating buffer preparation.
Low UV Absorbance: Low absorbance at 260 nm and 280 nm, suitable for experiments where UV transparency is important.

Applications

Preparation of alkaline buffers for enzyme assays (e.g., alkaline phosphatase).
Protein electrophoresis and separation, especially for proteins with high isoelectric points.
Electrotransfer / blotting onto PVDF or nitrocellulose membranes for protein analysis.
Protein sequencing, identification, or other protein-based assays requiring stable pH and minimal metal interaction.
Biophysical studies under alkaline buffer conditions, including membrane or nucleic acid assays.

Safety Disclaimer

This product is intended for laboratory research use only. Not for drug, human, or veterinary use. Review the Safety Data Sheet (SDS) provided by BioFargo for detailed handling, storage, hazard, and disposal information. Use appropriate personal protective equipment (PPE) including gloves, lab coat, and eye protection when handling. Dispose of waste and residual buffer in accordance with local, state, and federal regulations. Ensure proper ventilation if preparing large volumes or heating solutions.

Documents

SDS

CAPSO 99% Biochemical Grade