Description

Canine Bone Marrow Monocytes from Cell Biologics are isolated from beagle dog tibias and femurs. Cells at passage 0 are cryo-preserved and each vial contains 3x10⁶ cells per ml. Cells in suspension culture can be used for designed experiments under the cell culture conditions specified by Cell Biologics. Cells can be used in standard biochemical procedures include FACS, PCR, Western blotting, immunoprecipitation, ROS, or cell derivatives for desired research applications. Cells are negative for bacteria, yeast, fungi, and mycoplasma. These cells will not proliferate in culture and cannot be passaged.

Suggested Medium

M3366 Complete Leukocyte Medium w/ Kit (500 ml)

Storage

Cryopreserved Cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.

Documents

Cell-Culture-Protocol-CB

D-6271

Disclaimer

Investigators should handle the cells with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.