{"product_id":"can-get-signal-immunoreaction-enhancer-solution","title":"Can Get Signal Immunoreaction Enhancer Solution-Toyobo","description":"\u003cdiv style=\"font-family: 'Helvetica Neue', Helvetica, Arial, sans-serif; color: #333; line-height: 1.6; max-width: 1200px; margin: 0 auto; padding: 10px;\"\u003e\n\u003cdiv style=\"margin-bottom: 30px;\"\u003e\n\u003ch2 style=\"color: #003366;  padding-left: 15px; font-size: 24px; margin-bottom: 20px; font-weight: bold;\"\u003eDescription\u003c\/h2\u003e\n\u003cdiv style=\"background-color: #f9f9f9; padding: 20px; border-radius: 4px; border: 1px solid #eee;\"\u003e\n\u003cp style=\"margin-top: 0;\"\u003eCan Get Signal™ is a solution containing an accelerator for antigen-antibody reactions. This reagent improves sensitivity, specificity, and signal-to-noise ration (S\/N) for Western blotting, dot blotting, enzyme-linked immunosorbent assay (ELISA), etc. Solutions 1 and 2 refer to the reactions of the primary and secondary antibodies, respectively.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"margin-bottom: 30px;\"\u003e\n\u003ch2 style=\"color: #003366;  padding-left: 15px; font-size: 24px; margin-bottom: 20px; font-weight: bold;\"\u003eFeatures\u003c\/h2\u003e\n\u003cul style=\"list-style-type: none; padding-left: 0;\"\u003e\n\u003cli style=\"margin-bottom: 15px; padding-left: 25px; position: relative;\"\u003e\n\u003cspan style=\"position: absolute; left: 0; color: #003366;\"\u003e•\u003c\/span\u003e Enhances immunoassay signals up to several dozen times by maintaining low background signals.\u003c\/li\u003e\n\u003cli style=\"margin-bottom: 15px; padding-left: 25px; position: relative;\"\u003e\n\u003cspan style=\"position: absolute; left: 0; color: #003366;\"\u003e•\u003c\/span\u003e Can be used in combination with secondary antibodies labeled with peroxidase or alkaline phosphatase, etc.\u003c\/li\u003e\n\u003cli style=\"margin-bottom: 15px; padding-left: 25px; position: relative;\"\u003e\n\u003cspan style=\"position: absolute; left: 0; color: #003366;\"\u003e•\u003c\/span\u003e Can be used directly without dilution (ready-to-use).\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cdiv style=\"text-align: center; margin: 20px 0;\"\u003e\u003cimg style=\"max-width: 100%; height: auto;\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/pic_01_pc.png?v=1775542482\" alt=\"\"\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"margin-bottom: 30px;\"\u003e\n\u003ch2 style=\"color: #003366;  padding-left: 15px; font-size: 24px; margin-bottom: 20px; font-weight: bold;\"\u003eApplication\u003c\/h2\u003e\n\u003cp style=\"padding-left: 5px;\"\u003e・Western blotting, dot blotting\u003cbr\u003e・Enzyme-linked immunosorbent assay (ELISA),\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"margin-bottom: 30px;\"\u003e\n\u003ch2 style=\"color: #003366;  padding-left: 15px; font-size: 24px; margin-bottom: 20px; font-weight: bold;\"\u003eSpecification\u003c\/h2\u003e\n\u003cdiv style=\"overflow-x: auto; margin-bottom: 20px;\"\u003e\n\u003ctable style=\"width: 100%; border-collapse: collapse; border: 1px solid #e0e0e0; min-width: 600px;\"\u003e\n\u003cthead\u003e\n\u003ctr style=\"background-color: #003366; color: #ffffff;\"\u003e\n\u003cth style=\"padding: 12px; border: 1px solid #e0e0e0; text-align: left;\"\u003eCategory\u003c\/th\u003e\n\u003cth style=\"padding: 12px; border: 1px solid #e0e0e0; text-align: left;\"\u003eItem\u003c\/th\u003e\n\u003cth style=\"padding: 12px; border: 1px solid #e0e0e0; text-align: left;\"\u003eDetails\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0; font-weight: bold; background-color: #fcfcfc;\"\u003eStorage condition\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0; text-align: center;\"\u003e-\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003eStore at 4ºC\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch2 style=\"color: #003366;  padding-left: 15px; font-size: 24px; margin-bottom: 20px; font-weight: bold;\"\u003eComponents\u003c\/h2\u003e\n\u003cp\u003eThis kit includes the following components. All reagents should be stored at 4ºC, and protected from light.\u003c\/p\u003e\n\u003cdiv style=\"overflow-x: auto; margin-bottom: 20px;\"\u003e\n\u003ctable style=\"width: 100%; border-collapse: collapse; border: 1px solid #e0e0e0; min-width: 800px;\"\u003e\n\u003cthead\u003e\n\u003ctr style=\"background-color: #003366; color: #ffffff;\"\u003e\n\u003cth style=\"padding: 12px; border: 1px solid #e0e0e0; text-align: center;\" rowspan=\"2\"\u003eReagent Name\u003c\/th\u003e\n\u003cth style=\"padding: 12px; border: 1px solid #e0e0e0; text-align: center;\" colspan=\"4\"\u003eCode No.\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"background-color: #003366; color: #ffffff;\"\u003e\n\u003cth style=\"padding: 12px; border: 1px solid #e0e0e0; text-align: center;\"\u003eNKB-101T\u003c\/th\u003e\n\u003cth style=\"padding: 12px; border: 1px solid #e0e0e0; text-align: center;\"\u003eNKB-101\u003c\/th\u003e\n\u003cth style=\"padding: 12px; border: 1px solid #e0e0e0; text-align: center;\"\u003eNKB-201\u003c\/th\u003e\n\u003cth style=\"padding: 12px; border: 1px solid #e0e0e0; text-align: center;\"\u003eNKB-301\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0; text-align: center;\"\u003eSolution 1\u003cbr\u003efor primary antibody\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0; text-align: center;\"\u003e50 mL\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0; text-align: center;\"\u003e250 mL\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0; text-align: center;\"\u003e250 mL\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0; text-align: center;\"\u003e-\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"background-color: #fafafa;\"\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0; text-align: center;\"\u003eSolution 2\u003cbr\u003efor secondary antibody\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0; text-align: center;\"\u003e50 mL\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0; text-align: center;\"\u003e250 mL\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0; text-align: center;\"\u003e-\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0; text-align: center;\"\u003e250 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch2 style=\"color: #003366;  padding-left: 15px; font-size: 24px; margin-bottom: 20px; font-weight: bold;\"\u003eTypical reaction flow\u003c\/h2\u003e\n\u003cdiv style=\"text-align: center; margin: 20px 0;\"\u003e\u003cimg style=\"max-width: 100%; height: auto;\" alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/pic_02_pc.png?v=1775542799\"\u003e\u003c\/div\u003e\n\u003cp\u003eFlow chart of western blotting with Can Get Signal™\u003c\/p\u003e\n\u003cdiv style=\"text-align: center; margin: 20px 0;\"\u003e\u003cimg style=\"max-width: 100%; height: auto;\" alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/pic_03_pc.png?v=1775542798\"\u003e\u003c\/div\u003e\n\u003cp\u003eFlow chart of ELISA with Can Get Signal™\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"margin-bottom: 30px;\"\u003e\n\u003ch2 style=\"color: #003366;  padding-left: 15px; font-size: 24px; margin-bottom: 20px; font-weight: bold;\"\u003eApplication Data\u003c\/h2\u003e\n\u003cdiv style=\"margin-bottom: 40px; border: 1px solid #eee; padding: 20px; border-radius: 8px;\"\u003e\n\u003ch3 style=\"color: #333; font-size: 18px; margin-top: 0;\"\u003eExample 1.Detection of phosphorylated proteins by Western blotting\u003c\/h3\u003e\n\u003cp\u003ePhosphorylated Akt and ERK were detected by Western blotting analysis using Can Get Signal™ and a conventional method (TBS-T). As a result, the signal intensities of the target bands obtained with Can Get Signal™ were greater than those of the conventional method. The background level of the experiment with Can Get Signal™ was also significantly lower than that of the conventional method. The results suggest that Can Get Signal™ improves the sensitivity and specificity of Western blotting analysis.\u003c\/p\u003e\n\u003cdiv style=\"text-align: center; margin: 20px 0;\"\u003e\u003cimg style=\"max-width: 100%; height: auto;\" alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/pic_04_pc.png?v=1775542861\"\u003e\u003c\/div\u003e\n\u003cp\u003eFig. 1. Detection of phosphorylated protein kinases (p-Akt, p-ERK1 and p-ERK2) by Western blotting with Can Get Signal™ and a conventional method\u003cbr\u003eSample:Cultured bovine adrenal medulla cells\u003cbr\u003e1. Control (H2O)\u003cbr\u003e2. Insulin (1 nM, stimulated for 5 min)\u003cbr\u003e3. Insulin (10 nM, stimulated for 5 min)\u003cbr\u003e4. Insulin (100 nM, stimulated for 5 min)\u003c\/p\u003e\n\u003cdiv style=\"text-align: center; margin: 20px 0;\"\u003e\u003cimg style=\"max-width: 100%; height: auto;\" alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/pic_05_pc.png?v=1775542861\"\u003e\u003c\/div\u003e\n\u003cp\u003e\u003cstrong\u003eAntibodies:\u003c\/strong\u003e\u003cbr\u003e\u003cstrong\u003e\u0026lt;p-Akt\u0026gt;\u003c\/strong\u003e\u003cbr\u003ePrimary antibody:Anti Phospho-Akt rabbit polyclonal antibody (1:2,000 dilutioin) Secondary antibody:Anti rabbit-HRP antibody (1:20,000 dilution)\u003cbr\u003e\u003cstrong\u003e\u0026lt;p-ERK\u0026gt;\u003c\/strong\u003e\u003cbr\u003ePrimary antibody:Anti Phospho-ERK monoclonal antibody (1:2,000 dilutioin) Secondary antibody:Anti mouse-HRP antibody (1:20,000 dilution)\u003c\/p\u003e\n\u003cp\u003e*The data was kindly provided by Dr. Yanagita from the Department of Pharmacology, Faculty of Medicine, University of Miyazaki.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"margin-bottom: 40px; border: 1px solid #eee; padding: 20px; border-radius: 8px;\"\u003e\n\u003ch3 style=\"color: #333; font-size: 18px; margin-top: 0;\"\u003eExample 2. Detection of His-tagged proteins by Western blotting\u003c\/h3\u003e\n\u003cp\u003eHis-tagged recombinant proteins were detected with Can Get Signal™ and a conventional method (TBS-T). Can Get Signal™ showed excellent greater sensitivity than the conventional method.\u003c\/p\u003e\n\u003cdiv style=\"text-align: center; margin: 20px 0;\"\u003e\u003cimg style=\"max-width: 100%; height: auto;\" alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/pic_06_pc.png?v=1775542940\"\u003e\u003c\/div\u003e\n\u003cp\u003e\u003cstrong\u003eFig. 2. Detection of His-tagged proteins by Western blotting\u003c\/strong\u003e\u003cbr\u003e1: 6xHis-Catalase fusion protein\u003cbr\u003e2: 6xHis-Catalase fusion protein\u003cbr\u003e3: 6xHis-c-fos\u003cbr\u003e4: 6xHis-c-jun\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eAntibodies:\u003c\/strong\u003e\u003cbr\u003ePrimary antibody:Anti his-tag rabbit polyclonal antibody (1:2,000 dilutioin) Secondary antibody:Anti rabbit-IgG-HRP antibody (1:20,000 dilution)\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"margin-bottom: 40px; border: 1px solid #eee; padding: 20px; border-radius: 8px;\"\u003e\n\u003ch3 style=\"color: #333; font-size: 18px; margin-top: 0;\"\u003eExample 3. Detection of His-tagged proteins by ELISA\u003c\/h3\u003e\n\u003cp\u003eSandwich ELISA (solid phase antibody: anti-ERK2 monoclonal antibody, primary antibody: anti-His tag polyclonal antibody, secondary antibody: anti-rabbit IgG-HRP antibody) was performed to detect his-tagged human MAP kinase (His-ERK2) synthesized by a cell-free protein synthesis system. Can Get Signal™ showed an excellent quantitative curve as a function of antigen concentration whereas the conventional method with TBS-T resulted in low signals.\u003c\/p\u003e\n\u003cdiv style=\"text-align: center; margin: 20px 0;\"\u003e\u003cimg style=\"max-width: 100%; height: auto;\" alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/pic_07_pc.png?v=1775543012\"\u003e\u003c\/div\u003e\n\u003cp\u003e\u003cstrong\u003eFig. 3. Detection of His-tagged proteins by sandwich ELISA\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"margin-bottom: 30px;\"\u003e\n\u003ch2 style=\"color: #003366;  padding-left: 15px; font-size: 24px; margin-bottom: 20px; font-weight: bold;\"\u003eDocuments\u003c\/h2\u003e\n\u003cdiv style=\"border: 1px solid #eee; background-color: #f9f9f9; padding: 24px; border-radius: 8px;\"\u003e\n\u003cdiv style=\"display: flex; flex-wrap: wrap; gap: 15px;\"\u003e\n\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/NKB-101MAN.pdf?v=1775798495\" rel=\"noopener noreferrer\" style=\"display: inline-block; background-color: #003366; color: #ffffff; text-decoration: none; padding: 12px 24px; border-radius: 4px; font-size: 15px; font-weight: bold;\" target=\"_blank\"\u003e Download Manual \u003c\/a\u003e \u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/NKB-1_2_3_SDS.pdf?v=1775798494\" rel=\"noopener noreferrer\" style=\"display: inline-block; background-color: #ffffff; color: #003366; text-decoration: none; padding: 12px 24px; border-radius: 4px; font-size: 15px; font-weight: bold; border: 1px solid #003366;\" target=\"_blank\"\u003e Download SDS \u003c\/a\u003e \n  \u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/NKB_Eng.pdf?v=1775798781\" rel=\"noopener noreferrer\" style=\"display: inline-block; background-color: #ffffff; color: #003366; text-decoration: none; padding: 12px 24px; border-radius: 4px; font-size: 15px; font-weight: bold; border: 1px solid #003366;\" target=\"_blank\"\u003e Download Flyer \u003c\/a\u003e \n  \u003ca href=\"https:\/\/biofargo.com\/pages\/contact-us?srsltid=AfmBOorrYySVXlrC6mOen85FBiTKooBmPEyrEjjsqXyC0kKPRm7fDFPV\" rel=\"noopener noreferrer\" style=\"display: inline-block; background-color: #ffffff; color: #003366; text-decoration: none; padding: 12px 24px; border-radius: 4px; font-size: 15px; font-weight: bold; border: 1px solid #003366;\" target=\"_blank\"\u003e Request COA \u003c\/a\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"Toyobo","offers":[{"title":"Solution1","offer_id":52417664286901,"sku":"NKB-201","price":156.48,"currency_code":"USD","in_stock":true},{"title":"Solution2","offer_id":52417664319669,"sku":"NKB-301","price":156.48,"currency_code":"USD","in_stock":true},{"title":"Solution1\u00262","offer_id":52417664352437,"sku":"NKB-101","price":276.13,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Can_Get_Signal_Immunoreaction_Enhancer_Solution-Toyobo.jpg?v=1777517729","url":"https:\/\/biofargo.com\/products\/can-get-signal-immunoreaction-enhancer-solution","provider":"Biofargo","version":"1.0","type":"link"}