{"product_id":"c57bl-6-mouse-lung-mesenchymal-stem-cells","title":"C57BL\/6 Mouse Lung Mesenchymal Stem Cells","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eMouse Lung Mesenchymal Stem Cells are derived from the lung tissues of pathogen-free laboratory adult mice. Mouse Lung Mesenchymal Stem Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 2 min and incubated in \u003ci\u003eCell Biologics’\u003c\/i\u003e Cell Culture Medium for 7-15 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1x10\u003csup\u003e6\u003c\/sup\u003e cells per ml and is delivered frozen. Mouse Lung Mesenchymal Stem Cells are negative for bacteria, yeast, fungi, and mycoplasma. Repeated freezing and thawing of cells is not recommended. Mouse Lung Mesenchymal Stem Cells are tested for expression of markers using antibodies, CD44, Sca-1 and CD29 positive by flow cytometry. Cells can be expanded 3-8 passages by the ratio of 1:3 under the cell culture conditions specified by \u003ci\u003eCell Biologics.\u003c\/i\u003e\u003c\/strong\u003e\u003cstrong\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003ch3\u003eSuggested Medium\u003c\/h3\u003e\n\u003cp\u003e  Mouse Mesenchymal Stem Cell Medium \/w Kit - 500 ml\u003c\/p\u003e\n\u003cp\u003eCatalog No. \u003cb\u003eM5567\u003c\/b\u003e\u003c\/p\u003e\n\u003cp\u003eCustom order (2-4 weeks)\u003c\/p\u003e\n\u003ch2\u003eLaboratory Applications\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eMouse Lung Mesenchymal Stem Cells\u003c\/strong\u003e can be used in assays of standard biochemical procedures performed with cell cultures include RT-PCR, Western blotting, immunoprecipitation, or immunofluorescent flow cytometry. \u003c\/p\u003e\n\u003ch2\u003eStorage\u003c\/h2\u003e\n\u003cp\u003e\u003ci\u003eCell Biologics\u003c\/i\u003e ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use. Live-cell shipment is also available on request.\u003c\/p\u003e\n\u003cp\u003eNever can primary cells be kept at -20 °C.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/files.biofargo.com\/cell-biologics\/pdfs\/C57-6211_Cell-Culture-Protocol-CB.pdf\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eCell-Culture-Protocol-CB\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003ch2\u003eDisclaimer\u003c\/h2\u003e\n\u003cp\u003eAlthough \u003cstrong\u003eMouse Lung Mesenchymal Stem Cells\u003c\/strong\u003e are isolated from laboratory mice testing pathogen-free, investigators should handle the cells that they receive from \u003cem\u003eCell Biologics\u003c\/em\u003e\u003cstrong\u003e \u003c\/strong\u003ewith caution, since no test procedure can completely guarantee the absence of infectious agents.\u003c\/p\u003e\n","brand":"Cell Biologics","offers":[{"title":"1 x 10^6","offer_id":52481630372021,"sku":"C57-6211","price":560.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/IMG_0033a-500x500_9695ca24-66a2-47b4-9387-59c72b9d349f.jpg?v=1778251889","url":"https:\/\/biofargo.com\/products\/c57bl-6-mouse-lung-mesenchymal-stem-cells","provider":"Biofargo","version":"1.0","type":"link"}