{"product_id":"c57bl-6-gfp-mouse-primary-thymus-epithelial-cells","title":"C57BL\/6-GFP Mouse Primary Thymus Epithelial Cells","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eC57BL\/6-GFP Mouse Primary Thymus Epithelial Cells from \u003ci\u003eCell Biologics\u003c\/i\u003e are isolated from C57BL\/6-GFP-Tg(CAG-EGFP)1Osb\/J mouse thymus tissue of pathogen-free laboratory mice. C57BL\/6-GFP Mouse Primary Thymus Epithelial Cells are grown in a T25 tissue culture flask pre-coated with gelatin-based coating solution for 2 min and incubated in \u003ci\u003eCell Biologics\u003c\/i\u003e’ Culture Complete Growth Medium for 3-5 days. Cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 0.5x10\u003csup\u003e6\u003c\/sup\u003e cells per ml and is delivered frozen. The method we use to isolate primary epithelial cells was developed based on a combination of established and our proprietary methods. These cells are pre-coated with EpCAM-1 (CD326) antibody, followed by the application of magnetic beads pre-coated with secondary antibody.\u003c\/p\u003e\n\u003cp\u003e\u003cb\u003eProduct Testing\u003c\/b\u003e\u003cb\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003cp\u003eC57BL\/6-GFP Mouse Primary Thymus Epithelial Cells are characterized by immunofluorescent staining with antibodies of E-cadherin (Catalog 610182, BD) or ZO-1 (Catalog 617300, Life Technology). C57BL\/6-GFP Mouse Primary Thymus Epithelial Cells are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded for 3-7 passages at a split ratio of 1:2 under the cell culture conditions specified by \u003ci\u003eCell Biologics\u003c\/i\u003e. Repeated freezing and thawing of cells is not recommended.\u003c\/p\u003e\n\u003ch3\u003eSuggested Medium\u003c\/h3\u003e\n\u003cp\u003e\u003cb\u003e\u003c\/b\u003e  Epithelial Cell Medium \/w Kit – 500 ml\u003c\/p\u003e\n\u003cp\u003eCatalog No. \u003cb\u003eM6621\u003c\/b\u003e\u003cb\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003ch2\u003eLaboratory Applications\u003c\/h2\u003e\n\u003cp\u003eC57BL\/6-GFP Mouse Primary Thymus Epithelial Cells can be used for the assay of cell-cell interaction, adhesion, PCR, Western blot, immunoprecipitation, immunofluorescent flow cytometry, or generating cell derivatives for desired research applications.\u003c\/p\u003e\n\u003ch2\u003eStorage\u003c\/h2\u003e\n\u003cp\u003e\u003ci\u003eCell Biologics\u003c\/i\u003e ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use. Live cell shipment is also available on request.\u003c\/p\u003e\n\u003cp\u003eNever can primary cells be kept at -20 °C.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/files.biofargo.com\/cell-biologics\/pdfs\/mGFP-6209_Cell-Culture-Protocol-CB.pdf\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eCell-Culture-Protocol-CB\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003ch2\u003eDisclaimer\u003c\/h2\u003e\n\u003cp\u003eAlthough C57BL\/6-GFP Mouse Primary Thymus Epithelial Cells are isolated from laboratory mice testing pathogen-free, investigators should handle the cells that they receive from \u003ci\u003eCell Biologics\u003c\/i\u003e with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.\u003c\/p\u003e\n","brand":"Cell Biologics","offers":[{"title":"Frozen Vial","offer_id":52481631518901,"sku":"mGFP-6209","price":590.0,"currency_code":"USD","in_stock":true},{"title":"T25 Flask","offer_id":52481631551669,"sku":"mGFP-6209-1","price":650.0,"currency_code":"USD","in_stock":true},{"title":"T75 Flask","offer_id":52481631584437,"sku":"mGFP-6209-2","price":850.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/10162012_015Ms.EpiC-500x500.jpg?v=1778249559","url":"https:\/\/biofargo.com\/products\/c57bl-6-gfp-mouse-primary-thymus-epithelial-cells","provider":"Biofargo","version":"1.0","type":"link"}