{"product_id":"c57bl-6-gfp-mouse-primary-pancreatic-fibroblasts","title":"C57BL\/6-GFP Mouse Primary Pancreatic Fibroblasts","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eC57BL\/6-GFP Mouse Primary Pancreatic Fibroblasts from \u003cem\u003eCell Biologics\u003c\/em\u003e\u003cstrong\u003e \u003c\/strong\u003eare isolated from C57BL\/6-Tg(CAG-EGFP)1Osb\/J mouse pancreatic tissue of pathogen-free laboratory mice. C57BL\/6-GFP Mouse Primary Pancreatic Fibroblasts are grown in T75 tissue culture flasks pre-coated with gelatin-based coating solution for 2 min and incubated in \u003cem\u003eCell Biologics\u003c\/em\u003e\u003cstrong\u003e \u003c\/strong\u003eCulture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1x10\u003csup\u003e6\u003c\/sup\u003e cells per ml and is delivered frozen. \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eProduct Testing\u003c\/strong\u003e\u003cstrong\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eC57BL\/6-GFP Mouse Primary Pancreatic Fibroblasts are tested for expression of marker using the antibody of anti-FSP1\/S100A4 (Millipore USA) by immunofluorescence staining. C57BL\/6-GFP Mouse Primary Pancreatic Fibroblasts are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by \u003ci\u003eCell Biologics. \u003c\/i\u003eRepeated freezing and thawing of cells is not recommended.\u003c\/p\u003e\n\u003ch3\u003eSuggested Medium\u003c\/h3\u003e\n\u003cp\u003e Fibroblast Medium \/w Kit - 500 ml\u003c\/p\u003e\n\u003cp\u003eCatalog No. \u003cb\u003eM2267\u003c\/b\u003e\u003c\/p\u003e\n\u003ch2\u003eLaboratory Applications\u003c\/h2\u003e\n\u003cp\u003eC57BL\/6-GFP Mouse Primary Pancreatic Fibroblasts can be used in assays of standard biochemical procedures performed with cell cultures include RT-PCR, Western blotting, immunoprecipitation, immunofluorescent flow cytometry, or generating cell derivatives for desired research applications.\u003c\/p\u003e\n\u003ch2\u003eStorage\u003c\/h2\u003e\n\u003cp\u003e\u003cb\u003e\u003ci\u003eCell Biologics\u003c\/i\u003e\u003c\/b\u003e ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use. Live-cell shipment is also available on request. Never can primary cells be kept at -20 °C.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/files.biofargo.com\/cell-biologics\/pdfs\/mGFP-6201_Cell-Culture-Protocol-CB.pdf\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eCell-Culture-Protocol-CB\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003ch2\u003eDisclaimer\u003c\/h2\u003e\n\u003cp\u003eAlthough C57BL\/6-GFP Mouse Pancreatic Fibroblasts are isolated from laboratory mice testing pathogen-free, investigators should handle the cells that they receive from \u003ci\u003eCell Biologics\u003c\/i\u003e with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.\u003c\/p\u003e\n","brand":"Cell Biologics","offers":[{"title":"Frozen Vial","offer_id":52481630765237,"sku":"mGFP-6201","price":590.0,"currency_code":"USD","in_stock":true},{"title":"T25 Flask","offer_id":52481630798005,"sku":"mGFP-6201-1","price":650.0,"currency_code":"USD","in_stock":true},{"title":"T75 Flask","offer_id":52481630830773,"sku":"mGFP-6201-2","price":750.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Fibroblasts-500x500_4d2fe6a4-a5ce-4e3d-8d33-f49bdb4940a2.jpg?v=1778249942","url":"https:\/\/biofargo.com\/products\/c57bl-6-gfp-mouse-primary-pancreatic-fibroblasts","provider":"Biofargo","version":"1.0","type":"link"}