Description

C57BL/6-GFP Mouse Primary Bladder Epithelial Cells from Cell Biologics are isolated from C57BL/6-GFP-Tg(CAG-EGFP)1Osb/J mouse bladder tissue of pathogen-free laboratory mice. C57BL/6-GFP Mouse Primary Bladder Epithelial Cells are grown in a T25 tissue culture flask pre-coated with gelatin-based coating solution for 2 min and incubated in Cell Biologics’ Culture Complete Growth Medium for 3-5 days. Cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 0.5x10⁶ cells per ml and is delivered frozen. The method we use to isolate primary epithelial cells was developed based on a combination of established and our proprietary methods. These cells are pre-coated with EpCAM-1 (CD326) antibody, followed by the application of magnetic beads pre-coated with secondary antibody.

Product Testing

C57BL/6-GFP Mouse Primary Bladder Epithelial Cells are characterized by immunofluorescent staining with antibodies of E-cadherin (Catalog 610182, BD) or ZO-1 (Catalog 617300, Life Technology). C57BL/6-GFP Mouse Primary Bladder Epithelial Cells are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded for 3-7 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells is not recommended.

Suggested Medium

Epithelial Cell Medium /w Kit – 500 ml

Catalog No. M6621

Laboratory Applications

C57BL/6-GFP Mouse Primary Bladder Epithelial Cells can be used for the assay of cell-cell interaction, adhesion, PCR, Western blot, immunoprecipitation, immunofluorescent flow cytometry, or generating cell derivatives for desired research applications.

Storage

Cell Biologics ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use. Live cell shipment is also available on request.

Never can primary cells be kept at -20 °C.

Documents

Cell-Culture-Protocol-CB

Disclaimer

Although C57BL/6-GFP Mouse Primary Bladder Epithelial Cells are isolated from laboratory mice testing pathogen-free, investigators should handle the cells that they receive from Cell Biologics with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.