{"product_id":"brown-norway-rat-primary-kidney-glomerular-endothelial-cells","title":"Brown Norway Rat Primary Kidney Glomerular Endothelial Cells","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eLewis Rat Primary Kidney Glomerular Endothelial Cells from \u003ci\u003eCell Biologics\u003c\/i\u003e are isolated from aorta of 4-8 week old laboratory Brown Norway rat. Rat Primary Kidney Glomerular Endothelial Cells are grown in T75 tissue culture flasks pre-coated with gelatin-based coating solution for 2 min and incubated in \u003ci\u003eCell Biologics\u003c\/i\u003e’ Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells at passage 3 are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1x10\u003csup\u003e6\u003c\/sup\u003e cells per ml and is delivered frozen. The method we use to isolate primary endothelial cells was developed based on a combination of established and our proprietary methods. These cells are pre-coated with PECAM-1 (CD31) antibody, followed by the application of magnetic beads pre-coated with secondary antibody.\u003c\/p\u003e\n\u003cp\u003e\u003cb\u003eProduct Testing\u003c\/b\u003e\u003c\/p\u003e\n\u003cp\u003eRat Primary Kidney Glomerular Endothelial Cells are tested for expression of markers using antibody, PECAM-1 Antibody (M-20, sc-1506, Santa Cruz) or ZO-1 Rabbit Polyclonal Antibody (Catalog No. 617300, Life Technologies) by immunofluorescence staining. Rat Primary Kidney Glomerular Endothelial Cells are also tested for uptake of Dil-Ac-LDL (Catalog No. L-35353, Invitrogen), a functional marker for endothelial cells. Rat Primary Kidney Glomerular Endothelial Cells are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded for 3-7 passages at a split ratio of 1:2 or 1:3 under the cell culture conditions specified by \u003ci\u003eCell Biologics.\u003c\/i\u003e Repeated freezing and thawing of cells is not recommended.\u003c\/p\u003e\n\u003ch3\u003eSuggested Medium\u003c\/h3\u003e\n\u003cp\u003eEndothelial Cell Medium \/w Kit – 500 ml\u003c\/p\u003e\n\u003cp\u003eCatalog No. \u003cb\u003eM1266\u003c\/b\u003e\u003c\/p\u003e\n\u003ch2\u003eLaboratory Applications\u003c\/h2\u003e\n\u003cp\u003eRat Primary Kidney Glomerular Endothelial Cells can be used in assays of cell-cell adhesion, migration, vascular tube formation, transendothelial resistance (TER). Standard biochemical procedures performed with cell cultures include RT-PCR, western blotting, immunoprecipitation, immunofluorescent staining, immunofluorescent flow cytometry, or generating cell derivatives for desired research applications.\u003c\/p\u003e\n\u003ch2\u003eStorage\u003c\/h2\u003e\n\u003cp\u003e\u003ci\u003eCell Biologics\u003c\/i\u003e ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen\u003c\/p\u003e\n\u003cp\u003e(-180 °C) until ready for experimental use. Live cell shipment is also available on request.\u003c\/p\u003e\n\u003cp\u003eNever can primary cells be kept at -20 °C.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/files.biofargo.com\/cell-biologics\/pdfs\/RA-6014G.BN_RA-6014G.BN.pdf\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eRA-6014G.BN\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/files.biofargo.com\/cell-biologics\/pdfs\/RA-6014G.BN_Cell-Culture-Protocol-CB.pdf\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eCell-Culture-Protocol-CB\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003ch2\u003eDisclaimer\u003c\/h2\u003e\n\u003cp\u003eInvestigators should handle the cells that they receive from \u003ci\u003eCell Biologics\u003c\/i\u003e with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.\u003c\/p\u003e\n","brand":"Cell Biologics","offers":[{"title":"Frozen Vail","offer_id":52481665106101,"sku":"RA-6014G.BN","price":580.0,"currency_code":"USD","in_stock":true},{"title":"T25 Flask","offer_id":52481665138869,"sku":"RA-6014G.BN-1","price":630.0,"currency_code":"USD","in_stock":true},{"title":"T75 Flask","offer_id":52481665171637,"sku":"RA-6014G.BN-2","price":890.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Image_1923_copy-500x500.jpg?v=1778252568","url":"https:\/\/biofargo.com\/products\/brown-norway-rat-primary-kidney-glomerular-endothelial-cells","provider":"Biofargo","version":"1.0","type":"link"}