{"product_id":"bradford-protein-assay-kit","title":"Bradford Protein Quantification Assay Kit","description":"\u003c!-- =========================\nPRODUCT DESCRIPTION\n========================= --\u003e\n\u003csection\u003e\n\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eBradford Protein Quantification Assay Kit is a rapid colorimetric protein assay designed for accurate protein concentration measurement in biological samples. During routine protein preparation, inaccurate protein quantification can lead to inconsistent sample loading, unreliable Western blot results, and poor reproducibility in SDS-PAGE or protein analysis workflows. This Bradford protein assay kit provides a simple and reliable method to determine total protein concentration by measuring the absorbance change caused by Coomassie Brilliant Blue G-250 binding to proteins.\u003c\/p\u003e\n\u003cp\u003eBased on the Bradford assay method, the kit utilizes Coomassie Brilliant Blue G-250 dye under acidic conditions. When the dye binds to proteins, the absorption maximum shifts from approximately 465 nm to 595 nm, and the increase in A595 absorbance is proportional to protein concentration within the linear detection range. Protein concentration can be calculated using a BSA standard curve, making it suitable for routine protein quantification applications.\u003c\/p\u003e\n\u003cp\u003eBradford Protein Quantification Assay Kit provides a fast and convenient workflow compatible with microplate readers and spectrophotometers. Compared with traditional protein concentration determination methods, this protein assay kit offers simple operation, rapid detection, and good compatibility with many commonly used reducing agents, making it suitable for Western blot sample preparation, SDS-PAGE analysis, immunoprecipitation workflows, and general biochemical research.\u003c\/p\u003e\n\u003c\/section\u003e\n\u003c!-- =========================\nSPECIFICATIONS\n========================= --\u003e\n\u003csection\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable cellspacing=\"0\" cellpadding=\"8\" border=\"1\" style=\"width: 90%; border-collapse: collapse;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eProduct Name\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eBradford Protein Quantification Assay Kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eProduct Type\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eBradford Protein Assay Kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eAssay Principle\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eCoomassie Brilliant Blue G-250 Dye Binding Method\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eDetection Method\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eColorimetric Protein Assay\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eDetection Wavelength\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e595 nm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eStandard Protein\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eBovine Serum Albumin (BSA)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eCapacity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e1000 Assays\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eProtein Quantification, Western Blot, SDS-PAGE, Protein Analysis\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eSample Types\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eCell Lysates, Tissue Extracts, Purified Proteins\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e2–8 °C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eShelf Life\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e12 Months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/section\u003e\n\u003c!-- =========================\nFEATURES\n========================= --\u003e\n\u003csection\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eRapid protein quantification:\u003c\/strong\u003e Provides a fast Bradford assay workflow with color development and absorbance measurement completed within minutes.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eCoomassie Brilliant Blue G-250 based detection:\u003c\/strong\u003e Uses the classic Bradford protein assay method for reliable protein concentration determination.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003e595 nm colorimetric detection:\u003c\/strong\u003e Quantifies protein concentration by measuring A595 absorbance using a microplate reader or spectrophotometer.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eBSA standard curve calculation:\u003c\/strong\u003e Includes BSA protein standard for generating accurate protein concentration calibration curves.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eSimple experimental workflow:\u003c\/strong\u003e Requires minimal sample preparation and is suitable for routine laboratory protein analysis.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eReducing agent compatibility:\u003c\/strong\u003e Compatible with commonly used reducing agents including β-mercaptoethanol and DTT within recommended concentrations.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/section\u003e\n\u003c!-- =========================\nAPPLICATIONS\n========================= --\u003e\n\u003csection\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cp\u003eBradford Protein Quantification Assay Kit is widely used for protein concentration measurement in molecular biology, cell biology, and biochemical research workflows.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eWestern Blot Protein Quantification:\u003c\/strong\u003e Determines protein concentration before SDS-PAGE loading to improve sample normalization and experimental consistency.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eSDS-PAGE Sample Preparation:\u003c\/strong\u003e Provides accurate protein measurement for electrophoresis experiments requiring equal protein loading.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eCell Lysate Protein Analysis:\u003c\/strong\u003e Measures total protein concentration from cultured cell extracts and biological samples.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eTissue Protein Extraction:\u003c\/strong\u003e Quantifies protein concentration from animal tissue lysates for downstream biochemical studies.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eProtein Purification Analysis:\u003c\/strong\u003e Determines protein recovery and concentration during purification workflows.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eImmunoprecipitation (IP) and Co-IP:\u003c\/strong\u003e Supports protein normalization before antibody-based enrichment experiments.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eMicroplate Reader Protein Assay:\u003c\/strong\u003e Suitable for high-throughput protein concentration analysis using 96-well plates.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eGeneral Biochemical Research:\u003c\/strong\u003e Used for routine total protein measurement and protein sample characterization.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/section\u003e\n\u003c!-- =========================\nPROTOCOL OVERVIEW\n========================= --\u003e\n\u003csection\u003e\n\u003ch2\u003eProtocol Overview\u003c\/h2\u003e\n\u003cp\u003eThe Bradford protein assay provides a simple workflow for protein concentration determination using a BSA standard curve.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003ePrepare BSA standards and dilute protein samples appropriately.\u003c\/li\u003e\n\u003cli\u003ePrepare 1× Bradford working solution by diluting concentrated G-250 staining reagent.\u003c\/li\u003e\n\u003cli\u003eAdd protein standards or samples into a microplate or cuvette.\u003c\/li\u003e\n\u003cli\u003eAdd Bradford working reagent and incubate at room temperature for color development.\u003c\/li\u003e\n\u003cli\u003eMeasure absorbance at 595 nm and calculate protein concentration using the standard curve.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eFor accurate measurement, avoid excessive concentrations of detergents. High levels of SDS, Triton X-100, or Tween detergents may interfere with Bradford dye binding and affect protein concentration calculation.\u003c\/p\u003e\n\u003c\/section\u003e\n\u003c!-- =========================\nORDERING INFORMATION\n========================= --\u003e\n\u003csection\u003e\n\u003ch2\u003eOrdering Information\u003c\/h2\u003e\n\u003ctable cellspacing=\"0\" cellpadding=\"8\" border=\"1\" style=\"width: 90%; border-collapse: collapse;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eSKU\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eProduct\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eSize\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eAP0561\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eBradford Protein Quantification Assay Kit\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e1000 Assays\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/section\u003e\n\u003c!-- =========================\nFAQ\n========================= --\u003e\n\u003csection\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eFAQ\u003c\/h2\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhat is the principle of the Bradford protein assay?\u003c\/summary\u003e\n\u003cp\u003eThe Bradford assay is based on the binding of Coomassie Brilliant Blue G-250 dye to proteins under acidic conditions. Protein binding changes the dye absorption spectrum, producing increased absorbance at 595 nm that is proportional to protein concentration.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhat wavelength is used for Bradford protein quantification?\u003c\/summary\u003e\n\u003cp\u003eBradford protein quantification is typically measured at 595 nm using a microplate reader or spectrophotometer. The absorbance value is used to calculate protein concentration based on a BSA standard curve.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eCan Bradford Protein Quantification Assay Kit be used before Western blot?\u003c\/summary\u003e\n\u003cp\u003eYes. Bradford protein assay is commonly used to determine protein concentration before Western blot and SDS-PAGE experiments, allowing researchers to normalize protein loading between samples.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhat protein standard is used for Bradford assay?\u003c\/summary\u003e\n\u003cp\u003eBovine Serum Albumin (BSA) is commonly used as the protein standard for Bradford assays. The included BSA standard can be used to generate a calibration curve for protein concentration calculation.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhich substances may interfere with Bradford protein assay?\u003c\/summary\u003e\n\u003cp\u003eHigh concentrations of detergents may interfere with Bradford dye binding. SDS should generally be below 0.1%, Triton X-100 below 0.1%, and Tween detergents below recommended limits for accurate detection.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eIs Bradford assay compatible with reducing agents?\u003c\/summary\u003e\n\u003cp\u003eBradford protein quantification has good compatibility with some reducing agents. β-mercaptoethanol and DTT can generally be tolerated within appropriate concentrations, but sample composition should be considered during assay optimization.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003c\/section\u003e\n\u003c!-- =========================\nPRODUCT SCHEMA\n========================= --\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003c!-- =========================\nFAQ SCHEMA\n========================= --\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"AccuRef Scientific","offers":[{"title":"1000Assay","offer_id":52776432107701,"sku":"AP0561","price":51.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/5_cb92a414-9582-49c3-b9ae-2d36789e5672.jpg?v=1783496602","url":"https:\/\/biofargo.com\/products\/bradford-protein-assay-kit","provider":"Biofargo","version":"1.0","type":"link"}